Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Vorinostat 1.0000 2.6667
uM
 48
hr
 2 2222 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 252.58 NGFR rabbit IgG(H+L) / donkey NGFR 61.42
A375-A2-rep2
D2
WM-115 Vemurafenib Vorinostat 1.0000 2.6667
uM
 48
hr
 2 1419 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.82 NGFR rabbit IgG(H+L) / donkey NGFR 531.22
WM115-A2-rep2
D2
WM-115 Vemurafenib Vorinostat 1.0000 2.6667
uM
 48
hr
 1 1431 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.45 NGFR rabbit IgG(H+L) / donkey NGFR 436.29
WM115-A2-rep1
D2
WM-115 Vemurafenib Vorinostat 1.0000 0.1067
uM
 48
hr
 2 1513 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.96 NGFR rabbit IgG(H+L) / donkey NGFR 1082.99
WM115-B2-rep2
D2
A-375 Vemurafenib Vorinostat 1.0000 0.1067
uM
 48
hr
 1 2971 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 321.51 NGFR rabbit IgG(H+L) / donkey NGFR 148.00
A375-B2-rep1
D2
WM-115 Vemurafenib Vorinostat 1.0000 0.5333
uM
 48
hr
 1 1611 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.96 NGFR rabbit IgG(H+L) / donkey NGFR 915.06
WM115-B1-rep1
D2
COLO 858 Vemurafenib Vorinostat 0.3160 0.5333
uM
 48
hr
 2 1077 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 189.76 NGFR rabbit IgG(H+L) / donkey NGFR 154.45
Colo858-B1-rep2
D2
COLO 858 Vemurafenib Vorinostat 0.3160 0.1067
uM
 48
hr
 1 1749 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 253.38 NGFR rabbit IgG(H+L) / donkey NGFR 166.28
Colo858-B2-rep1
D2
WM-115 Vemurafenib Vorinostat 1.0000 0.5333
uM
 48
hr
 2 1514 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.65 NGFR rabbit IgG(H+L) / donkey NGFR 957.47
WM115-B1-rep2
D2
WM-115 Vemurafenib Vorinostat 1.0000 0.1067
uM
 48
hr
 1 1612 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.39 NGFR rabbit IgG(H+L) / donkey NGFR 1125.00
WM115-B2-rep1
D2
COLO 858 Vemurafenib Vorinostat 0.3160 0.5333
uM
 48
hr
 1 1313 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 213.34 NGFR rabbit IgG(H+L) / donkey NGFR 151.95
Colo858-B1-rep1
D2
A-375 Vemurafenib Vorinostat 1.0000 2.6667
uM
 48
hr
 1 1890 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 229.09 NGFR rabbit IgG(H+L) / donkey NGFR 60.09
A375-A2-rep1
D2
A-375 Vemurafenib Vorinostat 1.0000 0.5333
uM
 48
hr
 2 2869 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 443.59 NGFR rabbit IgG(H+L) / donkey NGFR 119.80
A375-B1-rep2
D2
A-375 Vemurafenib Vorinostat 1.0000 0.5333
uM
 48
hr
 1 2811 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 411.35 NGFR rabbit IgG(H+L) / donkey NGFR 111.10
A375-B1-rep1
D2
COLO 858 Vemurafenib Vorinostat 0.3160 2.6667
uM
 48
hr
 1 329 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 200.63 NGFR rabbit IgG(H+L) / donkey NGFR 17.50
Colo858-A2-rep1
D2
A-375 Vemurafenib Vorinostat 1.0000 0.1067
uM
 48
hr
 2 2959 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.98 NGFR rabbit IgG(H+L) / donkey NGFR 129.76
A375-B2-rep2
D2
COLO 858 Vemurafenib Vorinostat 0.3160 2.6667
uM
 48
hr
 2 478 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 242.50 NGFR rabbit IgG(H+L) / donkey NGFR 28.47
Colo858-A2-rep2
D2
COLO 858 Vemurafenib Vorinostat 0.3160 0.1067
uM
 48
hr
 2 1954 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 264.42 NGFR rabbit IgG(H+L) / donkey NGFR 180.44
Colo858-B2-rep2
D2
A-375 Vemurafenib Veliparib 1.0000 0.5333
uM
 48
hr
 1 2207 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 158.60 NGFR rabbit IgG(H+L) / donkey NGFR 168.87
A375-B1-rep1
C2
WM-115 Vemurafenib Veliparib 1.0000 2.6667
uM
 48
hr
 1 1299 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.15 NGFR rabbit IgG(H+L) / donkey NGFR 1076.34
WM115-A2-rep1
C2
COLO 858 Vemurafenib Veliparib 0.3160 0.1067
uM
 48
hr
 2 2385 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 352.61 NGFR rabbit IgG(H+L) / donkey NGFR 183.60
Colo858-B2-rep2
C2
COLO 858 Vemurafenib Veliparib 0.3160 0.1067
uM
 48
hr
 1 1792 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.98 NGFR rabbit IgG(H+L) / donkey NGFR 153.52
Colo858-B2-rep1
C2
COLO 858 Vemurafenib Veliparib 0.3160 0.5333
uM
 48
hr
 1 1919 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 252.38 NGFR rabbit IgG(H+L) / donkey NGFR 162.60
Colo858-B1-rep1
C2
A-375 Vemurafenib Veliparib 1.0000 0.1067
uM
 48
hr
 2 2173 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.93 NGFR rabbit IgG(H+L) / donkey NGFR 165.85
A375-B2-rep2
C2
A-375 Vemurafenib Veliparib 1.0000 0.1067
uM
 48
hr
 1 2645 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.72 NGFR rabbit IgG(H+L) / donkey NGFR 170.56
A375-B2-rep1
C2
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  • 1 to 25 of 1098 records