Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib (+)-JQ1 0.3160 0.5333
uM
 48
hr
 1 1371 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 102.15 NGFR rabbit IgG(H+L) / donkey NGFR 16.33
Colo858-B1-rep1
C7
COLO 858 Vemurafenib I-BET151 0.3160 2.6667
uM
 48
hr
 2 1438 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 110.13 NGFR rabbit IgG(H+L) / donkey NGFR 13.48
Colo858-A2-rep2
F7
COLO 858 Vemurafenib (+)-JQ1 0.3160 0.1067
uM
 48
hr
 1 1564 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 110.51 NGFR rabbit IgG(H+L) / donkey NGFR 23.82
Colo858-B2-rep1
C7
COLO 858 Vemurafenib I-BET 0.3160 2.6667
uM
 48
hr
 1 2153 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.18 NGFR rabbit IgG(H+L) / donkey NGFR 12.87
Colo858-A2-rep1
E7
COLO 858 Vemurafenib (+)-JQ1 0.3160 0.5333
uM
 48
hr
 2 1702 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.67 NGFR rabbit IgG(H+L) / donkey NGFR 14.18
Colo858-B1-rep2
C7
WM-115 Vemurafenib Methylstat 1.0000 2.6667
uM
 48
hr
 2 860 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 112.41 NGFR rabbit IgG(H+L) / donkey NGFR 846.07
WM115-A2-rep2
F6
WM-115 Vemurafenib Pracinostat 1.0000 2.6667
uM
 48
hr
 1 784 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 114.81 NGFR rabbit IgG(H+L) / donkey NGFR 370.58
WM115-A2-rep1
F4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1026 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 115.53 NGFR rabbit IgG(H+L) / donkey NGFR 936.58
WM115-A2-rep1
H9
WM-115 Vemurafenib CX-5461 1.0000 0.5333
uM
 48
hr
 2 1172 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 115.71 NGFR rabbit IgG(H+L) / donkey NGFR 1084.50
WM115-B1-rep2
F8
WM-115 Vemurafenib CX-5461 1.0000 2.6667
uM
 48
hr
 2 1216 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 116.78 NGFR rabbit IgG(H+L) / donkey NGFR 1052.21
WM115-A2-rep2
F8
WM-115 Vemurafenib Rocilinostat 1.0000 2.6667
uM
 48
hr
 2 1454 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 116.84 NGFR rabbit IgG(H+L) / donkey NGFR 520.46
WM115-A2-rep2
B5
WM-115 Vemurafenib Methylstat 1.0000 2.6667
uM
 48
hr
 1 908 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 117.67 NGFR rabbit IgG(H+L) / donkey NGFR 816.15
WM115-A2-rep1
F6
WM-115 Vemurafenib GSK-J2 1.0000 2.6667
uM
 48
hr
 2 1641 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 117.71 NGFR rabbit IgG(H+L) / donkey NGFR 1004.18
WM115-A2-rep2
C6
WM-115 Vemurafenib Rocilinostat 1.0000 2.6667
uM
 48
hr
 1 1297 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.10 NGFR rabbit IgG(H+L) / donkey NGFR 554.39
WM115-A2-rep1
B5
A-375 Vemurafenib (+)-JQ1 1.0000 0.5333
uM
 48
hr
 2 2283 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.15 NGFR rabbit IgG(H+L) / donkey NGFR 74.90
A375-B1-rep2
C7
WM-115 Vemurafenib Methylstat 1.0000 0.5333
uM
 48
hr
 2 1188 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.50 NGFR rabbit IgG(H+L) / donkey NGFR 1096.56
WM115-B1-rep2
F6
WM-115 Vemurafenib Pracinostat 1.0000 0.1067
uM
 48
hr
 2 1651 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.63 NGFR rabbit IgG(H+L) / donkey NGFR 885.63
WM115-B2-rep2
F4
WM-115 Vemurafenib GSK-J4 1.0000 2.6667
uM
 48
hr
 1 1461 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.76 NGFR rabbit IgG(H+L) / donkey NGFR 1082.05
WM115-A2-rep1
D6
COLO 858 Vemurafenib I-BET151 0.3160 2.6667
uM
 48
hr
 1 1486 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.87 NGFR rabbit IgG(H+L) / donkey NGFR 7.82
Colo858-A2-rep1
F7
WM-115 Vemurafenib Ischemin 1.0000 0.5333
uM
 48
hr
 2 1053 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.90 NGFR rabbit IgG(H+L) / donkey NGFR 1123.26
WM115-B1-rep2
G7
WM-115 Vemurafenib Entinostat 1.0000 2.6667
uM
 48
hr
 2 1505 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.16 NGFR rabbit IgG(H+L) / donkey NGFR 623.46
WM115-A2-rep2
D4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1118 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.18 NGFR rabbit IgG(H+L) / donkey NGFR 1039.92
WM115-B2-rep2
H9
WM-115 Vemurafenib GSK-J4 1.0000 2.6667
uM
 48
hr
 2 1545 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.21 NGFR rabbit IgG(H+L) / donkey NGFR 1027.05
WM115-A2-rep2
D6
WM-115 Vemurafenib Mocetinostat 1.0000 0.5333
uM
 48
hr
 2 1590 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.27 NGFR rabbit IgG(H+L) / donkey NGFR 1037.35
WM115-B1-rep2
E4
WM-115 Vemurafenib I-BET151 1.0000 2.6667
uM
 48
hr
 1 691 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.59 NGFR rabbit IgG(H+L) / donkey NGFR 497.01
WM115-A2-rep1
F7
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