Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1409 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.89 NGFR rabbit IgG(H+L) / donkey NGFR 1192.22
WM115-B2-rep1
A10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1121 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 328.09 NGFR rabbit IgG(H+L) / donkey NGFR 131.17
Colo858-B1-rep1
A10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1238 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.53 NGFR rabbit IgG(H+L) / donkey NGFR 1143.34
WM115-B1-rep1
A10
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1915 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 204.90 NGFR rabbit IgG(H+L) / donkey NGFR 199.36
A375-A2-rep2
A10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1307 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.08 NGFR rabbit IgG(H+L) / donkey NGFR 1224.07
WM115-B1-rep2
A10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1391 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.32 NGFR rabbit IgG(H+L) / donkey NGFR 158.87
Colo858-A2-rep1
A10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1149 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 377.09 NGFR rabbit IgG(H+L) / donkey NGFR 157.77
Colo858-B2-rep1
A10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1372 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.88 NGFR rabbit IgG(H+L) / donkey NGFR 973.52
WM115-A2-rep2
A10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1231 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 346.46 NGFR rabbit IgG(H+L) / donkey NGFR 151.76
Colo858-B1-rep2
A10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1100 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.13 NGFR rabbit IgG(H+L) / donkey NGFR 1179.05
WM115-B2-rep2
A10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1485 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 347.49 NGFR rabbit IgG(H+L) / donkey NGFR 160.81
Colo858-B2-rep2
A10
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1907 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 181.83 NGFR rabbit IgG(H+L) / donkey NGFR 159.59
A375-B1-rep1
A10
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1910 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.46 NGFR rabbit IgG(H+L) / donkey NGFR 175.04
A375-B2-rep2
A10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 988 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 341.76 NGFR rabbit IgG(H+L) / donkey NGFR 146.13
Colo858-A2-rep2
A10
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1834 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 165.77 NGFR rabbit IgG(H+L) / donkey NGFR 166.59
A375-A2-rep1
A10
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1697 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.98 NGFR rabbit IgG(H+L) / donkey NGFR 177.83
A375-B1-rep2
A10
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2268 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 202.77 NGFR rabbit IgG(H+L) / donkey NGFR 187.51
A375-B2-rep1
A10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1093 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.58 NGFR rabbit IgG(H+L) / donkey NGFR 1088.13
WM115-A2-rep1
A10
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1748 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.19 NGFR rabbit IgG(H+L) / donkey NGFR 174.69
A375-B1-rep1
A11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1440 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 346.44 NGFR rabbit IgG(H+L) / donkey NGFR 179.05
Colo858-B2-rep2
A11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1065 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 318.49 NGFR rabbit IgG(H+L) / donkey NGFR 135.54
Colo858-B2-rep1
A11
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1793 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.73 NGFR rabbit IgG(H+L) / donkey NGFR 197.31
A375-A2-rep2
A11
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1506 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.95 NGFR rabbit IgG(H+L) / donkey NGFR 162.28
A375-A2-rep1
A11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1350 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 353.28 NGFR rabbit IgG(H+L) / donkey NGFR 136.44
Colo858-B1-rep1
A11
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1596 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.87 NGFR rabbit IgG(H+L) / donkey NGFR 151.79
A375-B2-rep2
A11
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