Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib I-BET151 0.3160 2.6667
uM
 48
hr
 1 1486 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.87 NGFR rabbit IgG(H+L) / donkey NGFR 7.82
Colo858-A2-rep1
F7
COLO 858 Vemurafenib (+)-JQ1 0.3160 2.6667
uM
 48
hr
 1 1745 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.39 NGFR rabbit IgG(H+L) / donkey NGFR 10.46
Colo858-A2-rep1
C7
COLO 858 Vemurafenib I-BET 0.3160 2.6667
uM
 48
hr
 2 2077 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.89 NGFR rabbit IgG(H+L) / donkey NGFR 10.47
Colo858-A2-rep2
E7
COLO 858 Vemurafenib GSK-J4 0.3160 2.6667
uM
 48
hr
 2 682 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 189.00 NGFR rabbit IgG(H+L) / donkey NGFR 11.65
Colo858-A2-rep2
D6
COLO 858 Vemurafenib (+)-JQ1 0.3160 2.6667
uM
 48
hr
 2 1599 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.86 NGFR rabbit IgG(H+L) / donkey NGFR 11.70
Colo858-A2-rep2
C7
COLO 858 Vemurafenib Belinostat 0.3160 0.5333
uM
 48
hr
 2 367 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 240.33 NGFR rabbit IgG(H+L) / donkey NGFR 12.62
Colo858-B1-rep2
C4
COLO 858 Vemurafenib Entinostat 0.3160 2.6667
uM
 48
hr
 2 2000 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.13 NGFR rabbit IgG(H+L) / donkey NGFR 12.84
Colo858-A2-rep2
D4
COLO 858 Vemurafenib I-BET 0.3160 2.6667
uM
 48
hr
 1 2153 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.18 NGFR rabbit IgG(H+L) / donkey NGFR 12.87
Colo858-A2-rep1
E7
COLO 858 Vemurafenib I-BET151 0.3160 2.6667
uM
 48
hr
 2 1438 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 110.13 NGFR rabbit IgG(H+L) / donkey NGFR 13.48
Colo858-A2-rep2
F7
COLO 858 Vemurafenib (+)-JQ1 0.3160 0.5333
uM
 48
hr
 2 1702 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.67 NGFR rabbit IgG(H+L) / donkey NGFR 14.18
Colo858-B1-rep2
C7
COLO 858 Vemurafenib GSK-J4 0.3160 2.6667
uM
 48
hr
 1 528 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 232.59 NGFR rabbit IgG(H+L) / donkey NGFR 14.66
Colo858-A2-rep1
D6
COLO 858 Vemurafenib I-BET151 0.3160 0.5333
uM
 48
hr
 2 1692 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 132.87 NGFR rabbit IgG(H+L) / donkey NGFR 15.18
Colo858-B1-rep2
F7
COLO 858 Vemurafenib Entinostat 0.3160 2.6667
uM
 48
hr
 1 2256 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.46 NGFR rabbit IgG(H+L) / donkey NGFR 15.79
Colo858-A2-rep1
D4
COLO 858 Vemurafenib I-BET151 0.3160 0.5333
uM
 48
hr
 1 1798 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 126.05 NGFR rabbit IgG(H+L) / donkey NGFR 16.08
Colo858-B1-rep1
F7
COLO 858 Vemurafenib (+)-JQ1 0.3160 0.5333
uM
 48
hr
 1 1371 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 102.15 NGFR rabbit IgG(H+L) / donkey NGFR 16.33
Colo858-B1-rep1
C7
COLO 858 Vemurafenib Vorinostat 0.3160 2.6667
uM
 48
hr
 1 329 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 200.63 NGFR rabbit IgG(H+L) / donkey NGFR 17.50
Colo858-A2-rep1
D2
COLO 858 Vemurafenib PFI-1 0.3160 2.6667
uM
 48
hr
 2 1014 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.85 NGFR rabbit IgG(H+L) / donkey NGFR 19.30
Colo858-A2-rep2
B7
A-375 Vemurafenib Belinostat 1.0000 0.5333
uM
 48
hr
 1 1911 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 160.06 NGFR rabbit IgG(H+L) / donkey NGFR 19.85
A375-B1-rep1
C4
COLO 858 Vemurafenib Azacitidine 0.3160 2.6667
uM
 48
hr
 1 840 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 198.97 NGFR rabbit IgG(H+L) / donkey NGFR 20.08
Colo858-A2-rep1
E2
A-375 Vemurafenib Belinostat 1.0000 0.5333
uM
 48
hr
 2 2075 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 151.98 NGFR rabbit IgG(H+L) / donkey NGFR 20.46
A375-B1-rep2
C4
COLO 858 Vemurafenib Belinostat 0.3160 2.6667
uM
 48
hr
 2 165 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 207.48 NGFR rabbit IgG(H+L) / donkey NGFR 20.78
Colo858-A2-rep2
C4
COLO 858 Vemurafenib Belinostat 0.3160 2.6667
uM
 48
hr
 1 209 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 309.25 NGFR rabbit IgG(H+L) / donkey NGFR 21.49
Colo858-A2-rep1
C4
A-375 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 1 279 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 395.49 NGFR rabbit IgG(H+L) / donkey NGFR 21.95
A375-A2-rep1
C4
COLO 858 Vemurafenib Mocetinostat 0.3160 2.6667
uM
 48
hr
 1 2109 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.83 NGFR rabbit IgG(H+L) / donkey NGFR 22.08
Colo858-A2-rep1
E4
COLO 858 Vemurafenib Mocetinostat 0.3160 2.6667
uM
 48
hr
 2 2331 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 165.13 NGFR rabbit IgG(H+L) / donkey NGFR 22.79
Colo858-A2-rep2
E4
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