Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib GSK-J1 1.0000 2.6667
uM
 48
hr
 2 2734 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 198.78 NGFR rabbit IgG(H+L) / donkey NGFR 231.75
A375-A2-rep2
B6
A-375 Vemurafenib (+)-JQ1 1.0000 2.6667
uM
 48
hr
 2 2251 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 165.40 NGFR rabbit IgG(H+L) / donkey NGFR 86.81
A375-A2-rep2
C7
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2033 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 217.52 NGFR rabbit IgG(H+L) / donkey NGFR 238.98
A375-A2-rep2
A6
A-375 Vemurafenib Rocilinostat 1.0000 2.6667
uM
 48
hr
 2 2940 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.54 NGFR rabbit IgG(H+L) / donkey NGFR 76.23
A375-A2-rep2
B5
A-375 Vemurafenib Rucaparib 1.0000 2.6667
uM
 48
hr
 2 2477 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.42 NGFR rabbit IgG(H+L) / donkey NGFR 200.11
A375-A2-rep2
C3
A-375 Vemurafenib GSK-J2 1.0000 2.6667
uM
 48
hr
 2 3289 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 175.33 NGFR rabbit IgG(H+L) / donkey NGFR 210.26
A375-A2-rep2
C6
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2412 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 231.59 NGFR rabbit IgG(H+L) / donkey NGFR 226.51
A375-A2-rep2
A2
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2194 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 194.15 NGFR rabbit IgG(H+L) / donkey NGFR 186.08
A375-A2-rep2
A5
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2409 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 178.97 NGFR rabbit IgG(H+L) / donkey NGFR 199.27
A375-A2-rep2
A9
A-375 Vemurafenib CTB 1.0000 2.6667
uM
 48
hr
 2 2634 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 183.35 NGFR rabbit IgG(H+L) / donkey NGFR 163.76
A375-A2-rep2
B4
A-375 Vemurafenib UNC669 1.0000 2.6667
uM
 48
hr
 2 2373 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 210.68 NGFR rabbit IgG(H+L) / donkey NGFR 209.48
A375-A2-rep2
B8
A-375 Vemurafenib Veliparib 1.0000 2.6667
uM
 48
hr
 2 2511 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 190.08 NGFR rabbit IgG(H+L) / donkey NGFR 181.11
A375-A2-rep2
C2
A-375 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 2 319 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 360.58 NGFR rabbit IgG(H+L) / donkey NGFR 26.94
A375-A2-rep2
C4
A-375 Vemurafenib Selisistat 1.0000 2.6667
uM
 48
hr
 2 3238 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 195.56 NGFR rabbit IgG(H+L) / donkey NGFR 217.60
A375-A2-rep2
C5
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1915 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 204.90 NGFR rabbit IgG(H+L) / donkey NGFR 199.36
A375-A2-rep2
A10
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1793 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.73 NGFR rabbit IgG(H+L) / donkey NGFR 197.31
A375-A2-rep2
A11
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2177 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 175.69 NGFR rabbit IgG(H+L) / donkey NGFR 218.26
A375-A2-rep2
A3
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2355 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 209.47 NGFR rabbit IgG(H+L) / donkey NGFR 230.86
A375-A2-rep2
A4
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1797 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 197.36 NGFR rabbit IgG(H+L) / donkey NGFR 224.46
A375-A2-rep2
A7
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1871 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.59 NGFR rabbit IgG(H+L) / donkey NGFR 210.13
A375-A2-rep2
A8
A-375 Vemurafenib Olaparib 1.0000 2.6667
uM
 48
hr
 2 2229 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.21 NGFR rabbit IgG(H+L) / donkey NGFR 210.77
A375-A2-rep2
B2
A-375 Vemurafenib Iniparib 1.0000 2.6667
uM
 48
hr
 2 2597 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 177.05 NGFR rabbit IgG(H+L) / donkey NGFR 176.99
A375-A2-rep2
B3
A-375 Vemurafenib PFI-1 1.0000 2.6667
uM
 48
hr
 2 2464 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.44 NGFR rabbit IgG(H+L) / donkey NGFR 154.43
A375-A2-rep2
B7
A-375 Vemurafenib Vorinostat 1.0000 2.6667
uM
 48
hr
 2 2222 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 252.58 NGFR rabbit IgG(H+L) / donkey NGFR 61.42
A375-A2-rep2
D2
A-375 Vemurafenib UNC1215 1.0000 2.6667
uM
 48
hr
 2 2981 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 185.94 NGFR rabbit IgG(H+L) / donkey NGFR 204.11
A375-A2-rep2
C8
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