Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib GSK-J1 1.0000 0.1067
uM
 48
hr
 2 1158 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.88 NGFR rabbit IgG(H+L) / donkey NGFR 1182.56
WM115-B2-rep2
B6
WM-115 Vemurafenib (+)-JQ1 1.0000 0.1067
uM
 48
hr
 2 1160 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.96 NGFR rabbit IgG(H+L) / donkey NGFR 695.15
WM115-B2-rep2
C7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1174 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.28 NGFR rabbit IgG(H+L) / donkey NGFR 1115.34
WM115-B2-rep2
A6
WM-115 Vemurafenib Rocilinostat 1.0000 0.1067
uM
 48
hr
 2 1382 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.42 NGFR rabbit IgG(H+L) / donkey NGFR 1083.28
WM115-B2-rep2
B5
WM-115 Vemurafenib Rucaparib 1.0000 0.1067
uM
 48
hr
 2 1286 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.71 NGFR rabbit IgG(H+L) / donkey NGFR 1110.95
WM115-B2-rep2
C3
WM-115 Vemurafenib GSK-J2 1.0000 0.1067
uM
 48
hr
 2 1417 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.53 NGFR rabbit IgG(H+L) / donkey NGFR 1067.45
WM115-B2-rep2
C6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1222 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 158.91 NGFR rabbit IgG(H+L) / donkey NGFR 1104.02
WM115-B2-rep2
A2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1049 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.43 NGFR rabbit IgG(H+L) / donkey NGFR 1010.51
WM115-B2-rep2
A5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1162 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.94 NGFR rabbit IgG(H+L) / donkey NGFR 1096.98
WM115-B2-rep2
A9
WM-115 Vemurafenib CTB 1.0000 0.1067
uM
 48
hr
 2 1217 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.89 NGFR rabbit IgG(H+L) / donkey NGFR 1057.42
WM115-B2-rep2
B4
WM-115 Vemurafenib UNC669 1.0000 0.1067
uM
 48
hr
 2 1142 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.65 NGFR rabbit IgG(H+L) / donkey NGFR 1180.01
WM115-B2-rep2
B8
WM-115 Vemurafenib Veliparib 1.0000 0.1067
uM
 48
hr
 2 1355 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.84 NGFR rabbit IgG(H+L) / donkey NGFR 1032.08
WM115-B2-rep2
C2
WM-115 Vemurafenib Belinostat 1.0000 0.1067
uM
 48
hr
 2 1389 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.46 NGFR rabbit IgG(H+L) / donkey NGFR 499.87
WM115-B2-rep2
C4
WM-115 Vemurafenib Selisistat 1.0000 0.1067
uM
 48
hr
 2 1468 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.56 NGFR rabbit IgG(H+L) / donkey NGFR 1089.03
WM115-B2-rep2
C5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1100 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.13 NGFR rabbit IgG(H+L) / donkey NGFR 1179.05
WM115-B2-rep2
A10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1179 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.88 NGFR rabbit IgG(H+L) / donkey NGFR 1171.65
WM115-B2-rep2
A11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1434 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.89 NGFR rabbit IgG(H+L) / donkey NGFR 1166.16
WM115-B2-rep2
A3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1183 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.49 NGFR rabbit IgG(H+L) / donkey NGFR 1100.47
WM115-B2-rep2
A4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1123 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.11 NGFR rabbit IgG(H+L) / donkey NGFR 1122.60
WM115-B2-rep2
A7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 956 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.27 NGFR rabbit IgG(H+L) / donkey NGFR 1161.55
WM115-B2-rep2
A8
WM-115 Vemurafenib Olaparib 1.0000 0.1067
uM
 48
hr
 2 1291 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.34 NGFR rabbit IgG(H+L) / donkey NGFR 1162.67
WM115-B2-rep2
B2
WM-115 Vemurafenib Iniparib 1.0000 0.1067
uM
 48
hr
 2 1353 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.10 NGFR rabbit IgG(H+L) / donkey NGFR 1083.77
WM115-B2-rep2
B3
WM-115 Vemurafenib PFI-1 1.0000 0.1067
uM
 48
hr
 2 1186 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.71 NGFR rabbit IgG(H+L) / donkey NGFR 1098.16
WM115-B2-rep2
B7
WM-115 Vemurafenib Vorinostat 1.0000 0.1067
uM
 48
hr
 2 1513 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.96 NGFR rabbit IgG(H+L) / donkey NGFR 1082.99
WM115-B2-rep2
D2
WM-115 Vemurafenib UNC1215 1.0000 0.1067
uM
 48
hr
 2 1460 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.83 NGFR rabbit IgG(H+L) / donkey NGFR 1111.05
WM115-B2-rep2
C8
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