Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib GSK-J1 1.0000 2.6667
uM
 48
hr
 1 1200 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.71 NGFR rabbit IgG(H+L) / donkey NGFR 1023.37
WM115-A2-rep1
B6
WM-115 Vemurafenib (+)-JQ1 1.0000 2.6667
uM
 48
hr
 1 521 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.85 NGFR rabbit IgG(H+L) / donkey NGFR 452.89
WM115-A2-rep1
C7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1003 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.27 NGFR rabbit IgG(H+L) / donkey NGFR 1025.62
WM115-A2-rep1
A6
WM-115 Vemurafenib Rocilinostat 1.0000 2.6667
uM
 48
hr
 1 1297 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.10 NGFR rabbit IgG(H+L) / donkey NGFR 554.39
WM115-A2-rep1
B5
WM-115 Vemurafenib Rucaparib 1.0000 2.6667
uM
 48
hr
 1 1345 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.87 NGFR rabbit IgG(H+L) / donkey NGFR 1031.56
WM115-A2-rep1
C3
WM-115 Vemurafenib GSK-J2 1.0000 2.6667
uM
 48
hr
 1 1446 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.27 NGFR rabbit IgG(H+L) / donkey NGFR 1076.83
WM115-A2-rep1
C6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1314 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 162.02 NGFR rabbit IgG(H+L) / donkey NGFR 966.82
WM115-A2-rep1
A2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 929 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 151.87 NGFR rabbit IgG(H+L) / donkey NGFR 964.82
WM115-A2-rep1
A5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 996 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.05 NGFR rabbit IgG(H+L) / donkey NGFR 1083.30
WM115-A2-rep1
A9
WM-115 Vemurafenib CTB 1.0000 2.6667
uM
 48
hr
 1 1239 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.21 NGFR rabbit IgG(H+L) / donkey NGFR 813.08
WM115-A2-rep1
B4
WM-115 Vemurafenib UNC669 1.0000 2.6667
uM
 48
hr
 1 1162 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.02 NGFR rabbit IgG(H+L) / donkey NGFR 1039.91
WM115-A2-rep1
B8
WM-115 Vemurafenib Veliparib 1.0000 2.6667
uM
 48
hr
 1 1299 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.15 NGFR rabbit IgG(H+L) / donkey NGFR 1076.34
WM115-A2-rep1
C2
WM-115 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 1 705 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.59 NGFR rabbit IgG(H+L) / donkey NGFR 342.51
WM115-A2-rep1
C4
WM-115 Vemurafenib Selisistat 1.0000 2.6667
uM
 48
hr
 1 1455 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.38 NGFR rabbit IgG(H+L) / donkey NGFR 1061.79
WM115-A2-rep1
C5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1093 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.58 NGFR rabbit IgG(H+L) / donkey NGFR 1088.13
WM115-A2-rep1
A10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1094 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.45 NGFR rabbit IgG(H+L) / donkey NGFR 1030.60
WM115-A2-rep1
A11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1054 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.53 NGFR rabbit IgG(H+L) / donkey NGFR 926.14
WM115-A2-rep1
A3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1158 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.15 NGFR rabbit IgG(H+L) / donkey NGFR 1015.40
WM115-A2-rep1
A4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 930 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.08 NGFR rabbit IgG(H+L) / donkey NGFR 1077.20
WM115-A2-rep1
A7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 997 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.02 NGFR rabbit IgG(H+L) / donkey NGFR 955.42
WM115-A2-rep1
A8
WM-115 Vemurafenib Olaparib 1.0000 2.6667
uM
 48
hr
 1 1273 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.00 NGFR rabbit IgG(H+L) / donkey NGFR 997.40
WM115-A2-rep1
B2
WM-115 Vemurafenib Iniparib 1.0000 2.6667
uM
 48
hr
 1 1294 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.06 NGFR rabbit IgG(H+L) / donkey NGFR 1014.90
WM115-A2-rep1
B3
WM-115 Vemurafenib PFI-1 1.0000 2.6667
uM
 48
hr
 1 1044 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.16 NGFR rabbit IgG(H+L) / donkey NGFR 698.55
WM115-A2-rep1
B7
WM-115 Vemurafenib Vorinostat 1.0000 2.6667
uM
 48
hr
 1 1431 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.45 NGFR rabbit IgG(H+L) / donkey NGFR 436.29
WM115-A2-rep1
D2
WM-115 Vemurafenib UNC1215 1.0000 2.6667
uM
 48
hr
 1 1451 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.72 NGFR rabbit IgG(H+L) / donkey NGFR 1069.96
WM115-A2-rep1
C8
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