Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MMAC-SF 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 1.0684 2.0408 0.5556 0.2242 0.2841 0.6211 0.7407 0.6452 1.2255 1.0373 1.0730 0.6494 0.6024 0.7813 0.2174 0.6897 0.3704 0.3571 1.0204 0.7463
MMAC-SF 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 1.1723 3.9062 0.6250 0.2532 0.4167 0.7194 0.9615 0.6410 1.5823 1.0395 1.4493 0.8197 0.5650 0.9346 0.2016 0.7407 0.4386 0.5988 1.1249 1.0030
MMAC-SF 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 1.1236 3.3333 0.7353 0.2475 0.3597 0.6135 0.9346 0.5495 1.4859 1.0695 1.4144 0.7143 0.4808 0.9174 0.2183 0.8130 0.5025 0.5780 1.1792 0.9804
MMAC-SF 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.8403 2.2472 0.5025 0.2105 0.2833 0.5525 0.6897 0.5076 1.1655 0.8850 0.9524 0.6711 0.4464 0.7143 0.2028 0.7194 0.3690 0.3704 0.9346 0.7463
MMAC-SF 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.9009 2.2936 0.5618 0.2252 0.3030 0.6135 0.7463 0.4739 1.1723 0.9524 1.0000 0.6757 0.5155 0.7246 0.2075 0.3937 0.4000 0.9901 0.7194
MMAC-SF 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 1.1148 3.7879 0.7042 0.2404 0.3817 0.6667 0.9901 0.6410 1.5748 1.0695 1.4104 0.8130 0.5780 1.0267 0.2088 0.7407 0.4329 0.5848 1.0309 1.0428
MMAC-SF 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 1.1390 3.5088 0.7143 0.2457 0.3984 0.6135 1.0638 0.5405 1.5106 1.0953 1.5129 0.7692 0.5525 0.9901 0.2208 0.7463 0.4739 0.6173 1.0858 1.0449
MMAC-SF 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 1.0438 2.4631 0.6211 0.2193 0.2778 0.5650 0.7143 0.5618 1.2077 0.9804 1.2136 0.7246 0.5128 0.8265 0.1972 0.8000 0.4082 0.4184 0.9901 0.7874
MMAC-SF 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 1.1442 2.6525 0.6897 0.2381 0.3484 0.6211 0.9346 0.5814 1.3459 1.0593 1.3210 0.8197 0.6329 0.8772 0.2088 0.7463 0.3831 0.5102 1.0730 0.9174
MMAC-SF 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 1.2270 3.6630 0.6024 0.2410 0.3968 0.6667 0.9615 0.6897 1.5480 1.0010 1.3624 0.6944 0.5495 0.8333 0.2247 0.6944 0.4762 0.5882 1.1905 1.0050
MMAC-SF 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 1.2151 3.3113 0.6803 0.2146 0.4202 0.6711 0.9615 0.5780 1.3141 1.0040 1.3831 0.8065 0.5848 0.8547 0.2165 0.7576 0.4405 0.6536 1.3004 1.0309
MMAC-SF 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 1.0309 2.4938 0.5714 0.2331 0.2817 0.5587 0.7519 0.6061 1.2970 0.8403 1.0753 0.7353 0.4951 0.8065 0.2062 0.7463 0.4149 0.4098 1.0163 0.7874
MMAC-SF 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 1.1136 2.7174 0.6024 0.2193 0.3448 0.6098 0.8403 0.6173 1.1455 0.9434 1.0638 0.7813 0.5128 0.7813 0.2364 0.7519 0.3968 0.4673 1.0718 0.8929
MMAC-SF 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 1.2469 3.5587 0.6849 0.2326 0.3802 0.6250 0.9346 0.6897 1.6260 1.0246 1.3532 0.8130 0.6098 0.8197 0.2165 0.7194 0.4695 0.5714 1.2005 0.9615
MMAC-SF 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 1.2821 3.5842 0.6711 0.2625 0.3968 0.6369 1.0537 0.6452 1.3947 1.0020 1.4556 0.8929 0.6024 0.2132 0.6993 0.4484 0.5650 1.1976 0.9901
MMAC-SF 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 1.1710 2.5641 0.6536 0.2203 0.2865 0.5435 0.6803 0.6410 1.2674 0.9259 1.1876 0.7042 0.5155 0.8197 0.1988 0.7874 0.3953 0.4167 1.1050 0.8850
MMAC-SF 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 1.3423 3.0303 0.6289 0.2364 0.3610 0.6849 0.9615 0.5882 1.1947 1.0917 1.3569 0.8475 0.6369 0.8333 0.2141 0.7937 0.3774 0.5155 1.1547 1.0695
MMAC-SF 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 1.2706 4.2017 0.6211 0.2667 0.4405 0.6757 0.9524 0.6757 1.7452 0.9901 1.4925 0.9009 0.6098 0.8547 0.2101 0.7246 0.4695 0.5952 1.1312 0.9709
MMAC-SF 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 1.2484 3.9370 0.7092 0.2618 0.3968 0.6711 1.1050 0.6711 1.6892 1.0267 1.4472 0.8850 0.5814 0.9259 0.2179 0.7634 0.5102 0.5882 1.2626 0.9901
MMAC-SF 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 1.0000 2.3364 0.4951 0.2188 0.2519 0.5952 0.6944 0.5952 1.2739 0.8621 0.9901 0.6803 0.5435 0.6410 0.1905 0.7463 0.4386 0.3509 0.9709 0.7813
MMAC-SF 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 1.0493 2.1692 0.5128 0.2294 0.2985 0.6452 0.7407 0.6211 1.2453 0.9524 1.0582 0.7692 0.5348 0.6849 0.2079 0.7634 0.4237 0.3922 1.0460 0.7752
MMAC-SF 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 1.2870 3.5587 0.6173 0.2353 0.3413 0.6711 0.8621 0.6369 1.6779 0.8929 1.3158 0.8621 0.6061 0.8696 0.2304 0.6993 0.4673 0.6061 1.0549 0.9709
MMAC-SF 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 1.2594 3.4014 0.6369 0.2551 0.3774 0.6711 1.0132 0.6944 1.5974 0.9009 1.3986 0.8696 0.6289 0.8929 0.2315 0.6849 0.4695 0.6944 1.1534 1.0000
MMAC-SF 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 1.0905 2.3529 0.5747 0.2439 0.2604 0.6993 0.5682 1.2240 0.8403 1.0111 0.7519 0.5465 0.7194 0.2012 0.7519 0.4566 0.3484 1.0142 0.8696
MMAC-SF 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 1.1891 2.4876 0.5714 0.2506 0.2941 0.6757 0.8850 0.6452 1.2853 0.9174 1.1751 0.8403 0.6211 0.7937 0.2174 0.7752 0.3984 0.4219 1.1947 0.8696