Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MMAC-SF 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0882 0.6667 0.0057 0.0089 0.0207 0.1078 0.1078 0.0286 0.3497 0.0445 0.0357 0.0350 0.0122 0.0280 0.0058 0.1344 0.0258 0.0087 0.1083 0.1368
MMAC-SF 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0729 0.6897 0.0046 0.0054 0.0142 0.0668 0.0643 0.0184 0.2519 0.0370 0.0378 0.0353 0.0161 0.0165 0.0056 0.0877 0.0230 0.0071 0.0931
MMAC-SF 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0704 0.5917 0.0108 0.0264 0.0752 0.0945 0.0159 0.3788 0.0441 0.0272 0.0314 0.0132 0.0192 0.0053 0.0916 0.0091 0.1146 0.1546
MMAC-SF 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0946 0.6369 0.0065 0.0098 0.0175 0.1120 0.1212 0.0342 0.2457 0.0552 0.0481 0.0335 0.0154 0.0299 0.1407 0.0314 0.0095 0.0877 0.2066
MMAC-SF 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0995 0.7143 0.0060 0.0081 0.0186 0.1333 0.0957 0.0352 0.3546 0.0537 0.0382 0.0376 0.0193 0.0339 0.0060 0.1587 0.0326 0.0084 0.1040 0.1799
MMAC-SF 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0838 0.4808 0.0043 0.0049 0.0131 0.0838 0.0593 0.0241 0.2416 0.0342 0.0421 0.0271 0.0133 0.0195 0.0050 0.0947 0.0184 0.0072 0.0784 0.1133
MMAC-SF 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0727 0.5208 0.0050 0.0074 0.0196 0.0834 0.0987 0.0206 0.3356 0.0426 0.0249 0.0292 0.0130 0.0194 0.0048 0.1059 0.0270 0.0083 0.0858 0.1414
MMAC-SF 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0750 0.5051 0.0046 0.0069 0.0142 0.0803 0.0700 0.0277 0.2188 0.0385 0.0382 0.0251 0.0124 0.0222 0.0055 0.1036 0.0194 0.0056 0.0788 0.1429
MMAC-SF 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0743 0.5465 0.0054 0.0070 0.0172 0.0923 0.0928 0.0238 0.2976 0.0385 0.0277 0.0289 0.0123 0.0239 0.0048 0.1104 0.0239 0.0063 0.0890 0.1321
MMAC-SF 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0747 0.8265 0.0048 0.0053 0.0142 0.0636 0.0543 0.0249 0.2688 0.0337 0.0518 0.0416 0.0187 0.0170 0.0053 0.0838 0.0255 0.0086 0.0834 0.1515
MMAC-SF 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0733 0.7143 0.0065 0.0110 0.0200 0.0696 0.0887 0.0253 0.3311 0.0552 0.0261 0.0482 0.0192 0.0168 0.0052 0.0913 0.0107 0.1056 0.1534
MMAC-SF 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0770 0.5587 0.0048 0.0080 0.0141 0.0865 0.0984 0.0262 0.2786 0.0409 0.0363 0.0271 0.0122 0.0204 0.0057 0.1023 0.0234 0.0078 0.0806 0.1980
MMAC-SF 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0837 0.6211 0.0050 0.0057 0.0157 0.0860 0.0687 0.0255 0.3195 0.0396 0.0312 0.0307 0.0136 0.0249 0.0042 0.1149 0.0236 0.0068 0.0855 0.1346
MMAC-SF 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0775 0.6289 0.0049 0.0052 0.0148 0.0805 0.0693 0.0227 0.2667 0.0332 0.0385 0.0333 0.0139 0.0195 0.0053 0.1000 0.0206 0.0098 0.0780 0.1642
MMAC-SF 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0771 0.5435 0.0051 0.0070 0.0188 0.0866 0.0835 0.0211 0.3571 0.0430 0.0295 0.0293 0.0131 0.0192 0.0047 0.1107 0.0267 0.0077 0.0859 0.1689
MMAC-SF 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0668 0.5618 0.0038 0.0064 0.0144 0.0760 0.0709 0.0226 0.2141 0.0331 0.0313 0.0238 0.0109 0.0196 0.0053 0.0924 0.0180 0.0074 0.0792 0.1513
MMAC-SF 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0723 0.5587 0.0045 0.0060 0.0162 0.0739 0.0770 0.0198 0.2695 0.0390 0.0265 0.0290 0.0133 0.0196 0.0042 0.0993 0.0223 0.0066 0.0878 0.1409
MMAC-SF 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0880 0.7813 0.0056 0.0073 0.0228 0.1010 0.0895 0.0186 0.3205 0.0404 0.0434 0.0400 0.0121 0.0258 0.0053 0.1395 0.0212 0.0092 0.0868 0.2793
MMAC-SF 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.7874 0.0279 0.1034 0.0228 0.0499 0.0294 0.0151 0.0258 0.0063 0.1567 0.0298 0.0121 0.3731
MMAC-SF 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0993 0.6579 0.0092 0.0116 0.0224 0.1312 0.1715 0.0380 0.3448 0.0658 0.0407 0.0331 0.0206 0.0313 0.1727 0.0431 0.0092 0.0931 0.2217
MMAC-SF 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.1185 0.0096 0.0100 0.0270 0.1575 0.1504 0.0354 0.3690 0.0613 0.0522 0.0445 0.0170 0.0386 0.0080 0.1845 0.0374 0.0100 0.1205 0.1927
MMAC-SF 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0695 0.6579 0.0048 0.0061 0.0177 0.0720 0.0890 0.0189 0.2410 0.0457 0.0294 0.0384 0.0151 0.0171 0.0051 0.1140 0.0230 0.0124 0.0782 0.1403
MMAC-SF 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0799 0.7042 0.0047 0.0078 0.0198 0.0660 0.0899 0.0189 0.3984 0.0450 0.0362 0.0434 0.0124 0.0178 0.0056 0.0987 0.0264 0.0086 0.1220 0.1425
MMAC-SF 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0823 0.0066 0.0060 0.0174 0.2174 0.1148 0.0273 0.2179 0.0389 0.0276 0.0263 0.0140 0.0227 0.0063 0.1271 0.0252 0.0090 0.0903 0.1761
MMAC-SF 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0927 0.7299 0.0057 0.0079 0.0250 0.1080 0.1229 0.0241 0.2577 0.0459 0.0388 0.0501 0.0123 0.0252 0.0061 0.1274 0.0232 0.0076 0.1116 0.1595