Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

 Full screen view
Filter results: >>>>
Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MMAC-SF 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0838 0.6135 0.0051 0.0061 0.0181 0.0734 0.0955 0.0207 0.2232 0.0399 0.0322 0.0315 0.0164 0.0193 0.0050 0.1007 0.0256 0.0115 0.0929 0.1425
MMAC-SF 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0783 0.6803 0.0063 0.0099 0.0236 0.0729 0.1003 0.0198 0.2933 0.0419 0.0288 0.0366 0.0129 0.0195 0.0057 0.1070 0.0287 0.0095 0.1048 0.1631
MMAC-SF 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0881 0.5263 0.0071 0.0082 0.0167 0.1072 0.0261 0.2703 0.0471 0.0456 0.0290 0.0158 0.0235 0.1318 0.0275 0.0093 0.0867 0.1745
MMAC-SF 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0925 0.7092 0.0051 0.0077 0.0171 0.1114 0.1025 0.0282 0.2994 0.0445 0.0296 0.0346 0.0137 0.0298 0.0050 0.1255 0.0206 0.0083 0.1007 0.1603
MMAC-SF 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0724 0.5051 0.0047 0.0057 0.0154 0.0718 0.0750 0.0203 0.2584 0.0416 0.0369 0.0354 0.0143 0.0196 0.0044 0.0988 0.0230 0.0092 0.0764 0.1410
MMAC-SF 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0826 0.5155 0.0049 0.0094 0.0190 0.0807 0.0865 0.0172 0.3690 0.0423 0.0302 0.0372 0.0121 0.0195 0.0052 0.1005 0.0228 0.0085 0.0908 0.1290
MMAC-SF 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0701 0.4546 0.0068 0.0058 0.0149 0.0752 0.0938 0.0225 0.1815 0.0378 0.0253 0.0155 0.0195 0.0060 0.0975 0.0197 0.0061 0.0759 0.1427
MMAC-SF 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0756 0.5319 0.0047 0.0078 0.0196 0.0854 0.0783 0.0237 0.2717 0.0418 0.0256 0.0305 0.0099 0.0229 0.0051 0.1040 0.0191 0.0075 0.0895 0.1167
MMAC-SF 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0678 0.7246 0.0042 0.0059 0.0224 0.0579 0.0555 0.0163 0.2571 0.0397 0.0343 0.0400 0.0168 0.0147 0.0043 0.0925 0.0244 0.0094 0.0933 0.1133
MMAC-SF 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0706 0.7353 0.0063 0.0108 0.0644 0.0801 0.0146 0.3968 0.0420 0.0260 0.0471 0.0153 0.0163 0.0056 0.0907 0.0333 0.0107 0.1094 0.1339
MMAC-SF 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0850 0.5435 0.0061 0.0079 0.0160 0.0929 0.1179 0.0303 0.2392 0.0464 0.0409 0.0325 0.0166 0.0263 0.1148 0.0269 0.0104 0.0782
MMAC-SF 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0923 0.6289 0.0054 0.0065 0.0164 0.1198 0.0791 0.0316 0.3215 0.0444 0.0405 0.0366 0.0170 0.0270 0.0047 0.1218 0.0227 0.0074 0.0925 0.1466
MMAC-SF 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0861 0.5102 0.0047 0.0058 0.0972 0.0720 0.0306 0.2488 0.0379 0.0477 0.0284 0.0155 0.0249 0.0061 0.1127 0.0227 0.0063 0.0810 0.1458
MMAC-SF 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0833 0.5208 0.0053 0.0074 0.0169 0.1052 0.1078 0.0284 0.3509 0.0387 0.0316 0.0292 0.0127 0.0241 0.0051 0.1152 0.0271 0.0066 0.0951 0.1585
MMAC-SF 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0682 0.4425 0.0043 0.0051 0.0174 0.0699 0.0650 0.0236 0.1838 0.0325 0.0327 0.0238 0.0110 0.0192 0.0054 0.0903 0.0264 0.0067 0.0742 0.1364
MMAC-SF 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0717 0.5000 0.0044 0.0059 0.0177 0.0775 0.0689 0.0217 0.2545 0.0341 0.0271 0.0304 0.0115 0.0204 0.0042 0.0993 0.0209 0.0061 0.0877 0.1186
MMAC-SF 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0750 0.7092 0.0042 0.0050 0.0163 0.0589 0.0494 0.0169 0.2257 0.0288 0.0447 0.0289 0.0115 0.0158 0.0050 0.0799 0.0192 0.0066 0.0756 0.1295
MMAC-SF 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0698 0.4878 0.0051 0.0085 0.0193 0.0656 0.0855 0.0150 0.3356 0.0377 0.0247 0.0394 0.0128 0.0154 0.0051 0.0911 0.0270 0.0101 0.0864 0.1376
MMAC-SF 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0762 0.5714 0.0049 0.0071 0.0168 0.0885 0.1117 0.0251 0.3236 0.0533 0.0397 0.0337 0.0126 0.0198 0.0066 0.1063 0.0273 0.0098 0.0744 0.1776
MMAC-SF 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0867 0.6098 0.0048 0.0055 0.0166 0.1001 0.0762 0.0264 0.3356 0.0407 0.0340 0.0299 0.0148 0.0252 0.0042 0.1233 0.0242 0.0066 0.0859 0.1418
MMAC-SF 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0734 0.6579 0.0045 0.0061 0.0165 0.0662 0.0585 0.0165 0.3257 0.0347 0.0433 0.0325 0.0120 0.0169 0.0053 0.0859 0.0231 0.0073 0.1075 0.1592
MMAC-SF 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0870 0.6135 0.0051 0.0080 0.0183 0.0661 0.0797 0.0175 0.4831 0.0411 0.0401 0.0337 0.0124 0.0162 0.0047 0.1016 0.0282 0.0078 0.1052 0.1451
MMAC-SF 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0636 0.5128 0.0042 0.0058 0.0171 0.0702 0.0726 0.0183 0.2193 0.0307 0.0315 0.0277 0.0130 0.0158 0.0056 0.0831 0.0204 0.0061 0.0834 0.1460
MMAC-SF 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0657 0.5025 0.0043 0.0062 0.0189 0.0675 0.0762 0.0174 0.2667 0.0305 0.0264 0.0355 0.0101 0.0172 0.0039 0.0929 0.0242 0.0065 0.0861 0.1227
MMAC-SF 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0915 0.5495 0.0047 0.0066 0.0185 0.0948 0.0869 0.0188 0.3436 0.0283 0.0403 0.0314 0.0109 0.0232 0.0048 0.1312 0.0194 0.0081 0.1004