Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MMAC-SF 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 1.1641 4.1152 0.6849 0.2604 0.3745 0.6944 1.0395 0.7463 1.8450 1.1561 1.6234 0.6536 1.0194 0.2257 0.7194 0.4546 0.5780 1.3812 0.9259
MMAC-SF 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 1.1806 3.5088 0.7246 0.2494 0.3906 0.6369 0.9091 0.6494 1.7986 1.1547 1.5432 0.7937 0.5714 0.8772 0.2262 0.8547 0.5128 0.4695 1.3004 0.7752
MMAC-SF 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 1.1161 2.4631 0.5714 0.2273 0.3049 0.6211 0.7194 0.7194 1.4993 1.0406 1.2151 0.7813 0.6024 0.9346 0.2066 0.7463 0.3718 0.3937 1.1947 0.9615
MMAC-SF 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 1.1186 2.4038 0.5435 0.2310 0.3058 0.6944 0.8621 0.6897 1.4245 1.0183 1.2180 0.7042 0.6061 0.8000 0.2079 0.7519 0.3817 0.3937 1.2151 0.8621
MMAC-SF 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 1.1287 3.2895 0.6329 0.2755 0.3497 0.6494 0.8621 0.7143 1.7361 0.9615 1.4641 0.8197 0.6536 0.9091 0.2179 0.7634 0.4329 0.5208 1.1074 0.9615
MMAC-SF 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 1.1312 3.1447 0.7042 0.2451 0.3846 0.6410 0.9615 0.6579 1.6287 0.9615 1.4388 0.8333 0.5917 0.9259 0.2257 0.8130 0.4651 0.5208 1.0672 0.8197
MMAC-SF 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 1.1086 2.6667 0.6098 0.2755 0.2924 0.6211 0.7353 0.6369 1.4265 0.9346 1.1765 0.7874 0.6061 0.7937 0.2008 0.7353 0.4115 0.3968 1.0799 0.8333
MMAC-SF 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 1.2195 2.8249 0.5952 0.2475 0.3559 0.6667 0.8850 0.6173 1.4144 0.9709 1.2285 0.7634 0.6410 0.7519 0.2288 0.7519 0.4202 0.4566 1.1223 0.7752
MMAC-SF 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 1.1792 3.7037 0.6369 0.2519 0.3876 0.6711 0.9901 0.6173 1.7271 1.0953 1.4451 0.7937 0.5556 0.8621 0.2165 0.7299 0.4444 0.5208 1.1062 1.0121
MMAC-SF 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 1.1737 3.0960 0.6897 0.2416 0.3650 0.5882 0.8850 0.5025 1.4184 0.9615 1.3717 0.7407 0.4673 0.8696 0.2326 0.4808 0.5747 1.0695 0.8929
MMAC-SF 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.8403 1.9493 0.4587 0.2188 0.2558 0.5236 0.6173 0.4695 0.9615 0.7874 0.7692 0.5848 0.4505 0.6410 0.2079 0.7353 0.3521 0.3106 0.8130 0.7463
MMAC-SF 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.8929 1.9084 0.4673 0.1980 0.2463 0.5102 0.6993 0.4065 0.9804 0.8197 0.7937 0.6536 0.4405 0.6329 0.1919 0.6944 0.3677 0.3165 0.8475 0.7353
MMAC-SF 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 1.1723 3.6496 0.7042 0.2433 0.4132 0.6849 0.9804 0.6536 1.5152 1.0331 1.4430 0.8265 0.5556 0.8850 0.2364 0.7353 0.4255 0.5952 1.0493 0.9901
MMAC-SF 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 1.1211 3.3670 0.7299 0.2364 0.4098 0.5952 0.9804 0.5525 1.5291 1.0881 1.4620 0.8000 0.5405 0.9901 0.2237 0.7576 0.5000 0.5848 1.1013 0.8929
MMAC-SF 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.9615 2.4331 0.6452 0.2439 0.3195 0.5650 0.7407 0.5917 1.1806 0.9346 1.2240 0.7692 0.4926 0.8547 0.2110 0.7692 0.3953 0.4184 1.0471 0.8197
MMAC-SF 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 1.0881 2.6385 0.6897 0.2577 0.3367 0.6289 0.9091 0.6061 1.2579 1.0000 1.2610 0.8333 0.5747 0.8265 0.2262 0.7353 0.3731 0.4484 1.0941 0.8850
MMAC-SF 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 1.1848 3.1546 0.6250 0.2188 0.3236 0.6289 0.8772 0.6897 1.5848 0.9804 1.2019 0.7246 0.5650 0.8197 0.2066 0.7246 0.4255 0.4505 0.9901 0.9901
MMAC-SF 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 1.1710 2.8409 0.6250 0.2075 0.3497 0.5988 0.8197 0.5495 1.3004 0.9901 1.2438 0.8403 0.5587 0.9009 0.2008 0.4386 0.4484 1.0730 0.8621
MMAC-SF 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 1.0515 1.7452 0.4739 0.2439 0.5848 0.5882 0.5208 0.9901 0.9091 0.8130 0.6061 0.5181 0.7246 0.1883 0.6897 0.3636 0.3021 0.9524 0.7937
MMAC-SF 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 1.0627 1.7483 0.4785 0.2110 0.2703 0.5747 0.6329 0.5556 0.8547 0.9174 0.8621 0.6623 0.4951 0.6849 0.1949 0.7194 0.3636 0.3106 0.9615 0.8000
MMAC-SF 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 1.2658 3.5211 0.6803 0.2188 0.3584 0.6579 0.9346 0.6944 1.6863 1.0537 1.3755 0.8475 0.5917 0.9346 0.2049 0.7042 0.4587 0.5525 1.1025 1.0000
MMAC-SF 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 1.2953 3.4130 0.7299 0.2257 0.3891 0.6494 0.9901 0.6410 1.3928 1.0870 1.3812 0.8403 0.5882 0.8772 0.2088 0.7353 0.4444 0.5348 1.1429 0.9524
MMAC-SF 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 1.0953 1.9531 0.6098 0.2037 0.2347 0.6024 0.6024 0.5952 1.0537 0.9901 1.0246 0.6897 0.4951 0.8197 0.2024 0.7407 0.3677 0.3125 1.0515 0.8130
MMAC-SF 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 1.2547 2.1598 0.5780 0.2457 0.2611 0.6849 0.7407 0.6667 0.9709 1.0834 1.1351 0.7813 0.6098 0.7752 0.2037 0.7634 0.3497 0.3484 1.0627 1.0183
MMAC-SF 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.9009 2.0325 0.4505 0.2151 0.2336 0.5988 0.5952 0.5495 1.0627 0.8265 0.9346 0.6667 0.5051 0.6494 0.1961 0.7042 0.3953 0.2907 0.9174 0.6711