Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MMAC-SF 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.7874 1.9455 0.4032 0.1862 0.2160 0.5405 0.5747 0.4926 0.8696 0.9091 0.8929 0.5465 0.4032 0.5814 0.1828 0.7407 0.3390 0.3030 0.6711 0.6452
MMAC-SF 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.8929 2.0704 0.4167 0.2070 0.2410 0.5780 0.6897 0.4237 0.9615 0.9524 0.8265 0.6024 0.4608 0.5952 0.1890 0.6494 0.3788 0.3068 0.7634 0.7143
MMAC-SF 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 1.1547 4.1152 0.6494 0.2183 0.3984 0.7692 0.9346 0.6897 1.6949 1.1669 1.4205 0.7519 0.5650 0.8265 0.2174 0.6289 0.4762 0.5155 0.8772 1.0741
MMAC-SF 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 1.1521 3.8168 0.6536 0.2165 0.3718 0.7299 0.8850 0.6369 1.4006 1.1236 1.4245 0.8403 0.5319 0.9091 0.2110 0.7092 0.4951 0.5376 0.9259 0.9346
MMAC-SF 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.7874 1.9305 0.3718 0.2283 0.5236 0.5780 0.4386 0.9709 0.7634 0.6711 0.5495 0.3968 0.4878 0.1802 0.6993 0.3802 0.2967 0.6250 0.6993
MMAC-SF 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.8333 2.0450 0.3802 0.2075 0.2577 0.5376 0.6410 0.4184 0.8475 0.8333 0.7143 0.5848 0.4032 0.5102 0.1942 0.7194 0.3584 0.3311 0.6369 0.6452
MMAC-SF 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 1.1442 3.9216 0.6494 0.2188 0.3817 0.7463 0.9091 0.6369 1.5314 1.0776 1.3947 0.8403 0.5848 0.7752 0.2169 0.6289 0.4630 0.5435 1.0040 1.0050
MMAC-SF 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 1.1587 3.8760 0.6329 0.2075 0.3704 0.7937 1.0060 0.6623 1.3316 1.1351 1.4368 0.8850 0.5291 0.7692 0.2141 0.6849 0.4367 0.5319 1.0384 0.9434
MMAC-SF 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.9709 2.1008 0.4274 0.2475 0.2433 0.6369 0.5814 0.5051 0.9346 0.8475 0.8197 0.6211 0.4630 0.5952 0.2028 0.7299 0.3460 0.2985 0.7813 0.7874
MMAC-SF 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 1.0406 2.2075 0.4608 0.2494 0.2545 0.7463 0.6849 0.5587 0.9009 0.9091 0.9091 0.6494 0.5263 0.5747 0.2075 0.7092 0.3448 0.3030 0.8130 0.8333
MMAC-SF 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.9346 3.2787 0.5181 0.2151 0.3333 0.7042 0.8000 0.5814 1.4006 1.0406 1.1521 0.7692 0.5051 0.7407 0.1894 0.6803 0.4184 0.3968 0.8475 0.7634
MMAC-SF 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.9709 3.0769 0.5465 0.2222 0.3509 0.6757 0.9009 0.5814 1.3624 1.0331 1.1111 0.8000 0.4926 0.7692 0.2123 0.4167 0.3922 0.8696 0.8065
MMAC-SF 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.5882 1.4535 0.3236 0.2024 0.2033 0.3953 0.5051 0.3322 0.8403 0.5155 0.5208 0.4546 0.3115 0.4167 0.1852 0.6667 0.3636 0.2457 0.5236 0.6250
MMAC-SF 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.6211 1.4388 0.3247 0.2049 0.2114 0.4000 0.5682 0.3636 0.9091 0.5556 0.5556 0.4739 0.3205 0.4082 0.1880 0.6849 0.3546 0.2513 0.5208 0.4630
MMAC-SF 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.9804 2.9674 0.4274 0.2217 0.2577 0.6135 0.7246 0.5376 1.2563 0.9091 0.9615 0.6757 0.4926 0.5714 0.1969 0.6579 0.4202 0.3436 0.7813 0.8403
MMAC-SF 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 1.0460 2.3697 0.4484 0.1957 0.2809 0.6536 0.7576 0.5587 1.1669 0.9091 0.9524 0.7463 0.4386 0.6211 0.2165 0.6944 0.4184 0.3279 0.8265 0.8696
MMAC-SF 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
MMAC-SF 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
MMAC-SF 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 1.0846 2.7548 0.5814 0.2336 0.3215 0.7353 0.8333 0.6579 1.6667 0.9709 1.1947 0.7874 0.5988 0.8333 0.2028 0.7092 0.3704 0.4167 1.1696 0.9524
MMAC-SF 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 1.0091 2.5575 0.6135 0.2203 0.3345 0.6211 0.8197 0.6135 1.5528 0.9901 1.2315 0.7752 0.5780 0.8265 0.4425 0.4202 1.1792 0.8000
MMAC-SF 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.9091 2.0704 0.4808 0.2268 0.2865 0.5236 0.6803 0.5435 1.2723 0.8547 0.9615 0.6061 0.4926 0.6711 0.2070 0.7576 0.3846 0.3425 0.9524 0.6410
MMAC-SF 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.8850 2.0747 0.4808 0.2421 0.2778 0.5348 0.6711 0.5780 1.2594 0.8929 0.9346 0.6250 0.5128 0.6667 0.2083 0.7463 0.3831 0.4082 0.9615 0.6329
MMAC-SF 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 1.0638 2.5907 0.5988 0.2370 0.2985 0.5714 0.7752 0.6369 1.5221 0.9901 1.1820 0.7463 0.5814 0.8065 0.2137 0.7576 0.3802 0.4065 1.0267 1.0163
MMAC-SF 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 1.1111 2.4510 0.5917 0.2278 0.3226 0.6211 0.8403 0.6667 1.3850 1.0438 1.2563 0.7246 0.5682 0.8696 0.2033 0.8065 0.4167 0.4184 1.0504 0.8772
MMAC-SF 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 1.0060 1.8868 0.5236 0.2092 0.2326 0.4926 0.6173 0.5814 1.2077 1.0020 0.9804 0.6623 0.5236 0.7519 0.1938 0.7463 0.3677 0.3279 0.9804 0.7752