Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MMAC-SF 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 1.1962 3.7594 0.6536 0.2326 0.3690 0.7194 1.0081 0.6897 1.3369 1.1416 1.4859 0.8000 0.5405 0.7692 0.2203 0.6711 0.4184 0.5714 1.0000 1.0163
MMAC-SF 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.8772 1.8416 0.3953 0.2165 0.1946 0.5348 0.5319 0.4651 0.8929 0.8000 0.8265 0.5208 0.3937 0.5495 0.1832 0.7299 0.3534 0.2732 0.6757 0.6623
MMAC-SF 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.9434 2.0243 0.4386 0.2320 0.2532 0.6494 0.6250 0.4505 0.8265 0.9346 0.8850 0.5917 0.4695 0.5650 0.1961 0.6993 0.3636 0.3021 0.7353 0.7874
MMAC-SF 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 1.1074 3.6101 0.6061 0.2217 0.3378 0.7407 0.8333 0.6452 1.5291 1.1074 1.3550 0.8197 0.5291 0.8130 0.2114 0.6803 0.4425 0.5025 0.9346 0.9009
MMAC-SF 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 1.1074 3.4602 0.6135 0.2310 0.3774 0.7299 0.9901 0.6897 1.5015 1.0650 1.3774 0.8696 0.5714 0.8929 0.2227 0.7246 0.4505 0.5000 1.0030 0.9615
MMAC-SF 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.7246 1.9455 0.4167 0.2020 0.2066 0.4762 0.5747 0.4386 0.9804 0.7576 0.7463 0.6024 0.3906 0.5405 0.1818 0.6849 0.4000 0.2793 0.6211 0.6579
MMAC-SF 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.7874 1.8692 0.4219 0.2137 0.2481 0.5181 0.5952 0.4587 1.0121 0.7752 0.7874 0.6211 0.4049 0.5587 0.2132 0.7092 0.3876 0.2865 0.6623 0.5650
MMAC-SF 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 1.1236 3.4483 0.4854 0.2058 0.2770 0.6711 0.7752 0.5525 1.3699 0.9709 1.2346 0.8065 0.5263 0.6993 0.2101 0.6897 0.4854 0.4386 0.7752 0.9434
MMAC-SF 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 1.2092 3.0395 0.5348 0.2268 0.3460 0.6944 0.8696 0.6098 1.3193 1.0000 1.3532 0.8065 0.5465 0.7092 0.2232 0.6536 0.4608 0.4878 0.8547 0.8850
MMAC-SF 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.9434 2.1008 0.4630 0.1845 0.2410 0.5814 0.6061 0.4926 1.0288 0.8929 0.9259 0.6369 0.4673 0.6135 0.1901 0.6993 0.4202 0.2976 0.7634 0.7752
MMAC-SF 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.9901 2.3095 0.4329 0.1934 0.2463 0.6993 0.7092 0.5587 1.0753 1.0320 1.0091 0.7246 0.4785 0.6211 0.2119 0.6993 0.3759 0.3472 0.8265 0.8197
MMAC-SF 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 1.0309 3.8760 0.6369 0.3876 0.6849 0.9346 0.6623 1.7391 1.0537 1.4859 0.8403 0.6452 0.8696 0.6623 0.4505 0.4673 1.1198 0.8850
MMAC-SF 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 1.0718 3.3223 0.6452 0.2252 0.3378 0.6173 0.8547 0.5814 1.5924 1.0395 1.4430 0.7407 0.5682 0.7874 0.1912 0.8197 0.4329 1.0070 0.7143
MMAC-SF 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.8621 2.1368 0.4274 0.2128 0.2463 0.5495 0.6289 0.5102 1.1710 0.8000 0.8772 0.6061 0.4630 0.6410 0.1961 0.7576 0.3677 0.3509 0.7463 0.6993
MMAC-SF 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.8772 2.2422 0.4608 0.2062 0.2674 0.5882 0.7092 0.5319 1.1086 0.8333 0.8850 0.5814 0.5051 0.6369 0.2049 0.7634 0.3690 0.3584 0.7752 0.6452
MMAC-SF 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 1.1655 3.8314 0.6061 0.2364 0.3891 0.7407 0.9259 0.6944 1.7212 1.2136 1.5385 0.7813 0.5319 0.8696 0.1908 0.7407 0.4149 0.5291 0.9434 0.8850
MMAC-SF 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 1.1299 3.6101 0.6173 0.2041 0.4032 0.7246 0.9901 0.6667 1.5974 1.2034 1.4599 0.8333 0.5650 0.8696 0.2088 0.7874 0.4310 0.5076 0.9091 0.8929
MMAC-SF 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.8475 1.9194 0.4000 0.1969 0.2128 0.5556 0.5556 0.5000 1.0060 0.9091 0.8065 0.5988 0.4608 0.6061 0.1808 0.7042 0.3559 0.2825 0.7519 0.6849
MMAC-SF 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.8850 1.9881 0.4274 0.1996 0.2564 0.6250 0.6410 0.4785 1.0331 0.9091 0.8621 0.5208 0.5000 0.5952 0.1931 0.6849 0.3704 0.2793 0.7463 0.6536
MMAC-SF 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 1.1249 4.2194 0.5988 0.2410 0.4292 0.7299 0.9615 0.6494 1.6835 1.1834 1.4205 0.7576 0.5319 0.8772 0.2165 0.6803 0.4292 0.5495 0.8929 1.0320
MMAC-SF 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 1.0799 3.4364 0.6061 0.2062 0.3861 0.5952 0.8547 0.4525 1.3021 1.1455 1.4430 0.7576 0.4546 0.8197 0.1880 0.4546 0.4878 0.8547 0.9009
MMAC-SF 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.8065 2.4631 0.4464 0.1965 0.2475 0.5650 0.6849 0.5263 1.0070 0.8929 0.8547 0.5917 0.4310 0.6369 0.1883 0.7042 0.3817 0.2985 0.6410 0.6944
MMAC-SF 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.8696 2.3753 0.5000 0.1908 0.2849 0.5747 0.7246 0.4673 1.0672 0.9009 0.8850 0.6173 0.4717 0.6803 0.1916 0.6897 0.3774 0.3497 0.6711 0.7299
MMAC-SF 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 1.0384 3.3898 0.5348 0.2299 0.3401 0.7874 0.8475 0.5714 1.3123 1.1561 1.2920 0.7353 0.4444 0.6897 0.2012 0.6849 0.4115 0.4167 0.8772 0.8547
MMAC-SF 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.9709 3.1847 0.6061 0.2128 0.3509 0.6329 0.8929 0.4808 1.2063 1.0965 1.3106 0.6803 0.4975 0.6849 0.2141 0.7092 0.4444 0.4546 0.9009 0.8475