Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MMAC-SF 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0668 0.7634 0.0051 0.0073 0.0156 0.0682 0.0681 0.0219 0.5848 0.0414 0.0310 0.0433 0.0159 0.0223 0.0047 0.1178 0.0256 0.0091 0.0822 0.1808
MMAC-SF 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0833 0.8547 0.0056 0.0100 0.0159 0.1127 0.1087 0.0303 0.4425 0.0370 0.0460 0.0371 0.0189 0.0390 0.0086 0.1522 0.0312 0.0082 0.0821 0.2564
MMAC-SF 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0920 0.8621 0.0068 0.0084 0.0169 0.1217 0.1125 0.0326 0.6211 0.0423 0.0422 0.0460 0.0190 0.0426 0.0058 0.1678 0.0361 0.0093 0.0948 0.2457
MMAC-SF 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0638 0.7937 0.0039 0.0061 0.0154 0.0609 0.0500 0.0170 0.4425 0.0273 0.0365 0.0335 0.0178 0.0237 0.0050 0.0962 0.0243 0.0074 0.0817 0.1707
MMAC-SF 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0784 0.7463 0.0057 0.0106 0.0173 0.0574 0.0750 0.0178 0.6623 0.0365 0.0292 0.0471 0.0143 0.0221 0.0064 0.1002 0.0316 0.0098 0.0812 0.1969
MMAC-SF 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0990 0.8850 0.0086 0.0160 0.0226 0.1266 0.1718 0.0455 0.5917 0.0657 0.0485 0.0431 0.0232 0.0424 0.2110 0.0492 0.0103 0.1155 0.3012
MMAC-SF 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.1164 0.0098 0.0125 0.1548 0.1600 0.0393 0.5882 0.0635 0.0619 0.0237 0.0531 0.0075 0.2283 0.0407 0.0114 0.1395 0.2404
MMAC-SF 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0628 0.7143 0.0036 0.0064 0.0142 0.0689 0.0747 0.0180 0.3534 0.0351 0.0271 0.0336 0.0143 0.0212 0.0053 0.1083 0.0213 0.0083 0.0854 0.1812
MMAC-SF 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0706 0.6536 0.0042 0.0067 0.0148 0.0609 0.0794 0.0187 0.4902 0.0326 0.0385 0.0377 0.0138 0.0231 0.0048 0.1093 0.0227 0.0099 0.1027 0.1770
MMAC-SF 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0791 0.6667 0.0062 0.0069 0.0149 0.0862 0.1080 0.0284 0.4505 0.0340 0.0305 0.0330 0.0168 0.0311 0.0069 0.1553 0.0263 0.0096 0.0831 0.2475
MMAC-SF 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0891 0.7634 0.0062 0.0084 0.0176 0.1060 0.1050 0.0267 0.5155 0.0337 0.0422 0.0449 0.0145 0.0357 0.0061 0.1592 0.0234 0.0089 0.1020 0.1957
MMAC-SF 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0711 0.6711 0.0048 0.0056 0.0144 0.0666 0.0827 0.0269 0.3534 0.0371 0.0283 0.0341 0.0151 0.0255 0.0046 0.1107 0.0239 0.0109 0.0787 0.1585
MMAC-SF 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0702 0.6711 0.0056 0.0092 0.0156 0.0649 0.0889 0.0191 0.5348 0.0312 0.0295 0.0360 0.0136 0.0264 0.0055 0.1241 0.0278 0.0092 0.0906 0.1953
MMAC-SF 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0732 0.5917 0.0059 0.0099 0.0143 0.0887 0.1112 0.0264 0.3774 0.0396 0.0456 0.0309 0.0153 0.0260 0.0077 0.1409 0.0246 0.0091 0.0789 0.2024
MMAC-SF 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0819 0.8065 0.0046 0.0077 0.0169 0.0939 0.0831 0.0279 0.4808 0.0380 0.0308 0.0381 0.0146 0.0339 0.0044 0.1451 0.0181 0.0080 0.0858 0.1580
MMAC-SF 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0639 0.5291 0.0038 0.0053 0.0115 0.0560 0.0741 0.0197 0.3356 0.0261 0.0411 0.0339 0.0132 0.0233 0.0037 0.1086 0.0178 0.0066 0.0603 0.1585
MMAC-SF 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0622 0.6061 0.0040 0.0074 0.0151 0.0743 0.0858 0.0206 0.5348 0.0271 0.0294 0.0354 0.0114 0.0263 0.0046 0.1157 0.0170 0.0070 0.0862 0.1707
MMAC-SF 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0857 0.8000 0.0066 0.0079 0.0149 0.0951 0.1168 0.0339 0.3937 0.0442 0.0545 0.0360 0.0168 0.0382 0.0083 0.1529 0.0280 0.0082 0.0779 0.2058
MMAC-SF 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0961 0.9524 0.0059 0.0106 0.0207 0.1166 0.0992 0.0356 0.6369 0.0480 0.0373 0.0467 0.0158 0.0441 0.0063 0.1721 0.0257 0.0097 0.1043 0.1901
MMAC-SF 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0657 0.6667 0.0039 0.0057 0.0130 0.0569 0.0570 0.0216 0.4673 0.0280 0.0519 0.0366 0.0136 0.0233 0.0040 0.0987 0.0224 0.0086 0.0883 0.1227
MMAC-SF 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0605 0.9346 0.0053 0.0099 0.0177 0.0633 0.0791 0.0197 0.5556 0.0295 0.0330 0.0462 0.0128 0.0235 0.0052 0.1135 0.0299 0.0103 0.0975 0.1511
MMAC-SF 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0638 0.5587 0.0046 0.0080 0.0131 0.0690 0.0781 0.3472 0.0376 0.0354 0.0291 0.0129 0.0248 0.0059 0.1057 0.0206 0.0070 0.0611 0.1739
MMAC-SF 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0706 0.6993 0.0065 0.0047 0.0132 0.0783 0.0569 0.0249 0.4854 0.0417 0.0340 0.0317 0.0129 0.0281 0.0039 0.1212 0.0189 0.0058 0.0710
MMAC-SF 0.0000
uM
 10
hr
 7
InnoScan/Mapix
MMAC-SF 0.0000
uM
 10
hr
 7
InnoScan/Mapix