Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MMAC-SF 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.8850 3.3113 0.6173 0.2247 0.3546 0.5556 0.9174 0.6667 1.4577 0.9709 1.3624 0.7042 0.4673 0.8475 0.2053 0.5882 0.4082 0.4902 0.9524 0.7813
MMAC-SF 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 1.0309 3.9370 0.6993 0.2398 0.3984 0.6250 1.0000 0.6061 1.5361 0.9615 1.3298 0.8000 0.5465 0.8403 0.5556 0.4587 0.5682 1.0277 0.9434
MMAC-SF 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 1.0730 3.4722 0.5525 0.2141 0.3650 0.5780 0.9615 0.6579 1.3947 0.9259 1.4684 0.7937 0.4808 0.7752 0.1961 0.5495 0.4329 0.5618 1.0672 0.9615
MMAC-SF 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 1.0730 3.2468 0.6757 0.2237 0.3597 0.5348 1.0417 0.5435 1.4306 0.9346 1.4225 0.8130 0.4762 0.7692 0.2033 0.5952 0.4405 0.5525 1.1338 0.9901
MMAC-SF 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.8000 1.8450 0.4444 0.2008 0.2110 0.4673 0.5556 0.4951 0.9615 0.8547 0.5525 0.4219 0.6250 0.6061 0.3425 0.2833 0.8475 0.7353
MMAC-SF 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.9091 1.9724 0.4762 0.2004 0.2564 0.5405 0.7194 0.4831 1.0741 0.8772 0.8475 0.6494 0.4630 0.6536 0.1976 0.5747 0.3279 0.3509 0.9709 0.8333
MMAC-SF 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 1.2788 4.6083 0.5882 0.2123 0.4237 0.6173 0.9709 0.7092 1.7668 0.9615 1.4771 0.8547 0.5263 0.8929 0.2237 0.6098 0.4926 0.5988 1.1737 1.0152
MMAC-SF 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 1.3072 4.0816 0.6711 0.2320 0.4292 0.6250 1.0267 0.6803 1.5798 0.9709 1.4245 0.8547 0.5435 0.8850 0.2146 0.6329 0.4651 0.6410 1.2077 0.9901
MMAC-SF 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 1.0787 3.6496 0.5952 0.3236 0.5814 0.8130 0.6494 1.5015 0.8772 1.2407 0.6993 0.4831 0.7937 0.1976 0.6135 0.4505 0.4587 1.0000 0.8403
MMAC-SF 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 1.1507 3.8610 0.6173 0.2128 0.4000 0.6369 1.0060 0.6711 1.3870 0.9346 1.3550 0.8696 0.5525 0.8065 0.2545 0.5917 0.4237 0.5051 1.1050 0.9434
MMAC-SF 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 1.2755 4.3860 0.7246 0.2028 0.4367 0.7092 0.9524 0.6944 1.6447 0.9174 1.4925 0.8696 0.5556 0.8929 0.1949 0.5618 0.4717 0.6211 1.1351 1.0560
MMAC-SF 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 1.3532 4.3103 0.7092 0.2381 0.4386 0.6289 1.1198 0.7092 1.5152 0.9434 1.5060 0.8197 0.5988 0.8929 0.2188 0.5525 0.4608 0.7576 1.2547 1.1848
MMAC-SF 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 1.1481 2.9940 0.5376 0.2049 0.3003 0.5618 0.7143 0.5917 1.2469 0.8265 1.1696 0.7634 0.4310 0.7874 0.1866 0.6536 0.4149 0.4274 1.0256 1.0320
MMAC-SF 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 1.3351 3.4843 0.6667 0.2299 0.4016 0.7463 1.1111 0.7576 1.2240 1.0204 1.3369 0.8696 0.6623 0.7752 0.2062 0.6329 0.3745 0.5128 1.1820 1.2376
MMAC-SF 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 1.2594 4.4444 0.6410 0.2519 0.3937 0.6623 0.9615 0.6897 1.8051 1.0000 1.4749 0.8547 0.5348 0.8547 0.2268 0.6135 0.4651 0.5952 1.1223 0.9804
MMAC-SF 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 1.2270 4.1841 0.6289 0.2500 0.4115 0.6993 1.0941 0.6993 1.7825 0.9434 1.5175 0.8850 0.5618 0.8929 0.2088 0.6061 0.4878 0.5917 1.2376 1.0363
MMAC-SF 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.9804 2.9940 0.5435 0.1984 0.3205 0.5208 0.7634 0.6024 1.4409 0.8197 1.2422 0.7463 0.5181 0.7634 0.1934 0.6024 0.4405 0.4255 0.9901 0.8850
MMAC-SF 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 1.0846 2.8818 0.5714 0.2481 0.3788 0.5780 0.8929 0.6897 1.5337 0.8621 1.2887 0.8130 0.5495 0.7813 0.2198 0.6329 0.4274 0.4386 1.0953 0.8403
MMAC-SF 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 1.4025 4.8780 0.7407 0.2632 0.4329 0.6211 0.9524 0.7299 1.7182 0.9091 1.6694 0.9615 0.6623 0.9346 0.1949 0.4975 0.4717 0.7042 1.3387 1.3004
MMAC-SF 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 1.4620 4.8780 0.7576 0.2392 0.4808 0.7874 1.1820 0.8065 1.8051 1.0582 1.6026 0.9901 0.6849 0.8403 0.2092 0.5128 0.4762 0.7463 1.4327 1.2594
MMAC-SF 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 1.1351 3.5211 0.6061 0.2088 0.3774 0.6410 0.8130 0.6757 1.4903 0.9174 1.4451 0.7752 0.5102 0.8403 0.1972 0.6098 0.4739 0.4386 1.0929 1.0707
MMAC-SF 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 1.3986 4.0323 0.7246 0.2169 0.4049 0.8403 1.1274 0.7813 1.6393 1.0352 1.5480 0.8772 0.6536 0.8850 0.2315 0.6250 0.4167 0.5882 1.2422 1.0941
MMAC-SF 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 1.0341 4.2194 0.5848 0.2375 0.3704 0.6803 0.9709 0.6173 1.8484 1.1312 1.4493 0.7463 0.5780 0.9009 0.1927 0.5650 0.4329 0.5155 1.1274 0.9709
MMAC-SF 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 1.0929 3.3898 0.5952 0.2128 0.3367 0.5682 0.8696 0.5952 1.5038 1.1186 1.4286 0.6897 0.4854 0.7463 0.1905 0.5076 0.4695 1.0246 0.8065
MMAC-SF 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.9615 3.0675 0.5465 0.2141 0.2916 0.5917 0.7576 0.6173 1.5060 0.9709 1.1136 0.6711 0.5495 0.8000 0.1938 0.6098 0.3745 0.3597 0.9174 0.8772