Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

 Full screen view
Filter results: >>>>
Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MMAC-SF 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0840 0.8065 0.0051 0.0067 0.0160 0.1056 0.0726 0.0357 0.5376 0.0439 0.0446 0.0408 0.0156 0.0275 0.0050 0.1484 0.0223 0.0075 0.1111 0.1695
MMAC-SF 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0777 1.1364 0.0039 0.0063 0.0209 0.0796 0.0582 0.0211 0.4348 0.0461 0.0322 0.0550 0.0189 0.0193 0.0053 0.0979 0.0289 0.0089 0.0824 0.1302
MMAC-SF 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0700 1.2048 0.0046 0.0078 0.0263 0.0801 0.0783 0.0208 0.5988 0.0512 0.0224 0.0542 0.0190 0.0202 0.0044 0.1029 0.0399 0.0110 0.1189 0.1383
MMAC-SF 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0609 0.7194 0.0041 0.0058 0.0119 0.0622 0.0604 0.0238 0.3861 0.0361 0.0331 0.0277 0.0119 0.0214 0.0054 0.0991 0.0189 0.0100 0.0855 0.1486
MMAC-SF 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0661 0.7874 0.0041 0.0063 0.0130 0.0699 0.0610 0.0230 0.5525 0.0403 0.0344 0.0343 0.0140 0.0236 0.0043 0.1071 0.0201 0.0061 0.1003 0.1355
MMAC-SF 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0899 0.8850 0.0065 0.0181 0.0660 0.0625 0.0211 0.5747 0.0496 0.0457 0.0433 0.0123 0.0259 0.0065 0.1326 0.0213 0.0074 0.0965 0.1692
MMAC-SF 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0877 0.8621 0.0067 0.0238 0.0733 0.1073 0.0208 0.8197 0.0581 0.0323 0.0440 0.0152 0.0257 0.0063 0.1477 0.0313 0.0086 0.1147 0.2660
MMAC-SF 0.0000
uM
 48
hr
 8
InnoScan/Mapix
MMAC-SF 0.0000
uM
 48
hr
 8
InnoScan/Mapix
MMAC-SF 0.0000
uM
 48
hr
 8
InnoScan/Mapix
MMAC-SF 0.0000
uM
 48
hr
 8
InnoScan/Mapix
MMAC-SF 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0860 0.8850 0.0051 0.0080 0.0160 0.0848 0.1012 0.0240 0.5682 0.0506 0.0399 0.0402 0.0104 0.0264 0.0062 0.1412 0.0212 0.0065 0.0978 0.3559
MMAC-SF 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0826 0.9259 0.0058 0.0087 0.0182 0.0823 0.0988 0.0275 0.7692 0.0518 0.0344 0.0448 0.0116 0.0270 0.0054 0.1675 0.0238 0.0070 0.1127 0.2538
MMAC-SF 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0644 0.9434 0.0035 0.0028 0.0095 0.0131 0.0500 0.0197 0.0191 0.0073 0.0153 0.0339 0.0137 0.0223 0.0041 0.0198 0.0187 0.0037 0.0502 0.0488
MMAC-SF 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0638 0.9709 0.0040 0.0044 0.0097 0.0132 0.0739 0.0212 0.0303 0.0080 0.0156 0.0384 0.0146 0.0213 0.0047 0.0215 0.0275 0.0046 0.0608 0.0310
MMAC-SF 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 1.0428 4.3860 0.6623 0.2174 0.3968 0.6944 0.9709 0.7143 2.0325 1.0953 1.5576 0.7519 0.5952 0.8130 0.2101 0.4098 0.5208 1.1287 1.0449
MMAC-SF 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 1.0977 3.8911 0.6452 0.2359 0.4049 0.6369 0.9901 0.6173 1.6779 1.0846 1.6234 0.7246 0.5208 0.8333 0.2203 0.5076 0.5155 1.0672 0.9346
MMAC-SF 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.7246 2.0534 0.3861 0.1916 0.2227 0.4405 0.5988 0.4310 1.1481 0.7463 0.7692 0.5319 0.4049 0.5780 0.1678 0.5882 0.3311 0.2950 0.7576 0.6211
MMAC-SF 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.7576 2.0080 0.4082 0.2070 0.2506 0.4951 0.6098 0.4673 1.1641 0.8000 0.7874 0.4878 0.4367 0.5650 0.1946 0.6061 0.3311 0.3268 0.8000 0.5714
MMAC-SF 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 1.1025 3.9526 0.6536 0.2545 0.4016 0.5882 0.9009 0.6757 1.8315 0.9259 1.5038 0.7752 0.5814 0.8475 0.2045 0.5917 0.4219 0.5376 1.1038 1.0753
MMAC-SF 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 1.0604 3.8610 0.6250 0.2387 0.3831 0.5988 1.0571 0.6494 1.6340 0.9615 1.5949 0.7752 0.5650 0.8547 0.1789 0.5952 0.4082 0.5747 1.1173 1.0091
MMAC-SF 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.8850 2.4570 0.5128 0.2123 0.2933 0.5319 0.6993 0.6135 1.2270 0.8850 1.1547 0.6623 0.4975 0.7937 0.1667 0.5917 0.3690 0.3534 1.0000 0.8065
MMAC-SF 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 1.0142 2.6596 0.5882 0.2212 0.3521 0.6623 0.8621 0.6757 1.4104 0.9346 1.2165 0.6993 0.5952 0.7813 0.2037 0.5848 0.3484 0.4444 1.0638 0.8000
MMAC-SF 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 1.1947 4.7170 0.6250 0.2342 0.4132 0.6452 0.9901 0.6897 1.7794 1.0225 1.5175 0.8265 0.5155 0.8265 0.2070 0.5495 0.4386 0.6623 1.0277 1.0173
MMAC-SF 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 1.2121 3.8168 0.6579 0.2326 0.3846 0.6024 0.9615 0.5917 1.4286 0.9709 1.4641 0.7463 0.4255 0.9009 0.1957 0.6410 0.4762 0.6098 1.1628 1.0000