Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MMAC-SF 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.0797 0.7692 0.0072 0.0940 0.0320 0.5882 0.0264 0.0433 0.0366 0.0144 0.0268 0.0101 0.1661 0.0087 0.1002 0.3677
MMAC-SF 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0459 0.5208 0.0051 0.0055 0.0121 0.0435 0.0672 0.0394 0.4831 0.0239 0.0335 0.0246 0.0120 0.0056 0.0614 0.0221 0.0068 0.1515
MMAC-SF 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0527 0.6061 0.0053 0.0044 0.0112 0.0668 0.0492 0.0378 0.5181 0.0323 0.0289 0.0289 0.0109 0.0236 0.0048 0.0684 0.0225 0.0064 0.0559 0.0950
MMAC-SF 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0562 0.6410 0.0039 0.0047 0.0119 0.0649 0.0582 0.0185 0.3922 0.0218 0.0213 0.0272 0.0111 0.0219 0.0054 0.0907 0.0197 0.0056 0.0725 0.2294
MMAC-SF 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0598 0.6757 0.0042 0.0061 0.0126 0.0664 0.0785 0.0199 0.6061 0.0267 0.0237 0.0306 0.0099 0.0225 0.0055 0.1038 0.0226 0.0061 0.1550
MMAC-SF 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0588 0.6024 0.0047 0.0074 0.0137 0.0533 0.0748 0.4808 0.0220 0.0303 0.0284 0.0116 0.0215 0.1010 0.0198 0.0059 0.0650 0.3584
MMAC-SF 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0648 0.6897 0.0049 0.0067 0.0138 0.0556 0.0791 0.0246 0.5102 0.0259 0.0335 0.0100 0.0241 0.0051 0.1099 0.0212 0.0063 0.0641 0.2770
MMAC-SF 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0620 0.5376 0.0043 0.0040 0.0128 0.0706 0.0510 0.0153 0.4065 0.0241 0.0300 0.0288 0.0110 0.0224 0.0042 0.0867 0.0206 0.0058 0.0653 0.1499
MMAC-SF 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.0612 0.5556 0.0052 0.0065 0.0140 0.0662 0.0656 0.0157 0.5682 0.0276 0.0254 0.0337 0.0109 0.0204 0.0048 0.0923 0.0258 0.0648 0.1299
MMAC-SF 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0602 0.6849 0.0044 0.0072 0.0203 0.0602 0.0837 0.0167 0.4274 0.0331 0.0292 0.0358 0.0132 0.0199 0.0057 0.0998 0.0249 0.0094 0.0444 0.1883
MMAC-SF 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0702 0.8475 0.0049 0.0061 0.0168 0.0678 0.0169 0.4167 0.0382 0.0316 0.0456 0.0162 0.0238 0.0045 0.1151 0.0200 0.0075 0.0664 0.1120
MMAC-SF 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0605 0.7194 0.0044 0.0053 0.0160 0.0567 0.0819 0.0154 0.3610 0.0410 0.0235 0.0344 0.0142 0.0229 0.0048 0.1284 0.0205 0.0090 0.0581 0.1795
MMAC-SF 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0647 0.7519 0.0044 0.0065 0.0188 0.0554 0.0890 0.0148 0.5650 0.0388 0.0254 0.0413 0.0133 0.0248 0.0047 0.1427 0.0253 0.0098 0.0684 0.1919
MMAC-SF 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0646 0.8929 0.0044 0.0067 0.0196 0.0597 0.0781 0.0138 0.3861 0.0445 0.0266 0.0399 0.0181 0.0172 0.0067 0.1229 0.0248 0.0094 0.0501 0.1923
MMAC-SF 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0678 1.0000 0.0055 0.0063 0.0244 0.0581 0.0776 0.0139 0.5025 0.0487 0.0371 0.0514 0.0135 0.0254 0.0057 0.1100 0.0267 0.0092 0.0600 0.1570
MMAC-SF 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0637 0.7407 0.0043 0.0045 0.0178 0.0546 0.0696 0.0183 0.2725 0.0351 0.0250 0.0355 0.0119 0.0204 0.0042 0.1080 0.0195 0.0065 0.0554 0.1242
MMAC-SF 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0683 0.6897 0.0054 0.0068 0.0223 0.0551 0.0866 0.0149 0.4525 0.0361 0.0258 0.0376 0.0127 0.0209 0.0054 0.1170 0.0287 0.0085 0.0651 0.1616
MMAC-SF 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0626 0.5495 0.0051 0.0051 0.0132 0.0539 0.0718 0.0199 0.2994 0.0437 0.0368 0.0408 0.0207 0.0978 0.0180 0.0059 0.0463 0.1339
MMAC-SF 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0591 0.7143 0.0042 0.0060 0.0178 0.0567 0.0539 0.0216 0.4386 0.0482 0.0258 0.0415 0.0109 0.0242 0.0039 0.1074 0.0173 0.0077 0.0578 0.1064
MMAC-SF 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0513 0.5525 0.0033 0.0047 0.0124 0.0412 0.0534 0.0139 0.3436 0.0290 0.0296 0.0336 0.0107 0.0185 0.0030 0.1093 0.0146 0.0062 0.0435 0.1264
MMAC-SF 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0507 0.5882 0.0039 0.0076 0.0171 0.0490 0.0811 0.0132 0.5236 0.0305 0.0218 0.0371 0.0101 0.0198 0.0040 0.1212 0.0205 0.0073 0.0551 0.1351
MMAC-SF 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0521 0.5814 0.0035 0.0053 0.0142 0.0480 0.0684 0.0184 0.3356 0.0331 0.0378 0.0346 0.0105 0.0174 0.0049 0.0989 0.0170 0.0071 0.0466 0.1318
MMAC-SF 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0570 0.6944 0.0041 0.0087 0.0195 0.0582 0.0681 0.0174 0.4505 0.0367 0.0264 0.0336 0.0109 0.0202 0.0043 0.1038 0.0193 0.0070 0.0667 0.1238
MMAC-SF 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0584 0.7407 0.0045 0.0047 0.0183 0.0547 0.0601 0.0266 0.3571 0.0412 0.0354 0.0352 0.0119 0.0226 0.0043 0.1342 0.0173 0.0090 0.0553 0.1669
MMAC-SF 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0561 0.7092 0.0056 0.0091 0.0247 0.0650 0.0952 0.0240 0.5000 0.0469 0.0260 0.0402 0.0116 0.0230 0.0059 0.1433 0.0074 0.0620