Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MMAC-SF 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0672 0.7194 0.0046 0.0087 0.0149 0.0760 0.0815 0.0298 0.3205 0.0451 0.0429 0.0323 0.0137 0.0276 0.0071 0.1178 0.0267 0.0066 0.0464 0.2114
MMAC-SF 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0743 0.7937 0.0048 0.0063 0.0167 0.0927 0.0653 0.0304 0.4739 0.0436 0.0339 0.0350 0.0172 0.0313 0.0048 0.1311 0.0272 0.0075 0.0546 0.1653
MMAC-SF 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0592 0.5747 0.0040 0.0056 0.0163 0.0663 0.0598 0.0188 0.3311 0.0310 0.0437 0.0302 0.0173 0.0212 0.0048 0.0824 0.0170 0.0064 0.0463 0.1181
MMAC-SF 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0592 0.6667 0.0052 0.0071 0.0203 0.0612 0.0639 0.0193 0.4630 0.0443 0.0334 0.0319 0.0126 0.0191 0.0045 0.0925 0.0393 0.0075 0.0568 0.1650
MMAC-SF 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0733 0.7246 0.0047 0.0088 0.0166 0.0803 0.0743 0.0280 0.3367 0.0419 0.0432 0.0305 0.0146 0.0284 0.0074 0.1239 0.0257 0.0062 0.0600 0.1883
MMAC-SF 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0718 0.7874 0.0052 0.0077 0.0189 0.0906 0.0943 0.0311 0.4651 0.0394 0.0366 0.0334 0.0165 0.0327 0.0050 0.1368 0.0292 0.0071 0.0702 0.1715
MMAC-SF 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0805 0.9346 0.0064 0.0061 0.0204 0.0702 0.0765 0.0224 0.5556 0.0322 0.0409 0.0131 0.0281 0.0062 0.1555 0.0238 0.0083 0.0551 0.2227
MMAC-SF 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0831 0.9524 0.0791 0.0187 0.5917 0.0415 0.0364 0.0460 0.0148 0.0286 0.0069 0.1795 0.0355 0.0639
MMAC-SF 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0706 0.7353 0.0054 0.0062 0.0169 0.0826 0.0926 0.0282 0.4386 0.0409 0.0393 0.0323 0.0161 0.0270 0.0078 0.1175 0.0272 0.0067 0.0502 0.2525
MMAC-SF 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0775 0.8197 0.0052 0.0060 0.0181 0.0894 0.0689 0.0281 0.4673 0.0449 0.0369 0.0356 0.0177 0.0051 0.1427 0.0307 0.0077 0.0549 0.1934
MMAC-SF 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0626 0.6494 0.0050 0.0048 0.0161 0.0617 0.0587 0.0199 0.3236 0.0290 0.0380 0.0270 0.0121 0.0230 0.0065 0.0963 0.0213 0.0059 0.0517 0.2000
MMAC-SF 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0668 0.6536 0.0051 0.0063 0.0155 0.0631 0.0655 0.0222 0.5208 0.0397 0.0333 0.0312 0.0116 0.0227 0.0047 0.1120 0.0262 0.0074 0.0543 0.1698
MMAC-SF 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0622 0.7042 0.0043 0.0078 0.0170 0.0826 0.0754 0.0270 0.3257 0.0376 0.0395 0.0288 0.0140 0.0236 0.0069 0.1193 0.0249 0.0065 0.0482 0.2119
MMAC-SF 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0706 0.7092 0.0057 0.0065 0.0170 0.0876 0.0829 0.0270 0.4464 0.0448 0.0349 0.0452 0.0141 0.0257 0.0045 0.1253 0.0290 0.0074 0.0517 0.1942
MMAC-SF 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0597 0.8403 0.0041 0.0046 0.0216 0.0524 0.0516 0.0152 0.3068 0.0367 0.0302 0.0401 0.0155 0.0182 0.0044 0.0836 0.0242 0.0085 0.0651 0.1637
MMAC-SF 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0596 0.8475 0.0055 0.0090 0.0519 0.0960 0.0167 0.4587 0.0467 0.0248 0.0458 0.0153 0.0199 0.0055 0.0921 0.0334 0.0098 0.0641 0.2849
MMAC-SF 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0765 0.7407 0.0074 0.0114 0.0215 0.1079 0.1355 0.0346 0.4566 0.0492 0.0385 0.0365 0.0180 0.0319 0.0094 0.1650 0.0378 0.0087 0.0692 0.2976
MMAC-SF 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0864 1.0204 0.0070 0.0098 0.0230 0.1208 0.1182 0.0330 0.4484 0.0487 0.0465 0.0456 0.0192 0.0386 0.0062 0.1681 0.0296 0.0092 0.0861 0.1984
MMAC-SF 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0584 0.6410 0.0040 0.0067 0.0146 0.0611 0.0776 0.0182 0.0311 0.0265 0.0312 0.0141 0.0213 0.0055 0.1119 0.0232 0.0097 0.0489 0.1866
MMAC-SF 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0658 0.7246 0.0042 0.0061 0.0154 0.0665 0.0783 0.0167 0.4219 0.0350 0.0354 0.0496 0.0112 0.0240 0.0047 0.1159 0.0228 0.0087 0.0593 0.1799
MMAC-SF 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0794 0.8403 0.0095 0.0059 0.0200 0.1100 0.1272 0.0331 0.4167 0.0519 0.0398 0.0361 0.0183 0.0313 0.0089 0.1751 0.0321 0.0109 0.0520 0.2538
MMAC-SF 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0902 0.9615 0.0079 0.0084 0.0252 0.1163 0.1266 0.0326 0.4854 0.0551 0.0504 0.0438 0.0143 0.0338 0.0068 0.1822 0.0293 0.0106 0.0697 0.2146
MMAC-SF 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0830 0.9009 0.0052 0.0074 0.0211 0.0904 0.1244 0.0264 0.3831 0.0389 0.0416 0.0388 0.0190 0.0307 0.0055 0.1776 0.0233 0.0112 0.0763 0.2817
MMAC-SF 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0866 0.9009 0.0077 0.0121 0.0248 0.0905 0.1389 0.0257 0.0391 0.0453 0.0432 0.0160 0.0318 0.0073 0.1931 0.0292 0.0116 0.0928 0.3597
MMAC-SF 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0745 0.7353 0.0076 0.0072 0.0187 0.1015 0.1266 0.0319 0.3636 0.0534 0.0486 0.0355 0.0173 0.0304 0.0091 0.1548 0.0303 0.0101 0.0561 0.1992