Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MMAC-SF 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 1.0730 3.0211 0.5495 0.2222 0.3448 0.5747 0.9009 0.6410 1.4472 1.0000 1.1390 0.6803 0.5525 0.7299 0.2008 0.6173 0.3718 0.4082 0.9615 0.8475
MMAC-SF 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 1.3123 4.2918 0.6849 0.2545 0.4348 0.6494 0.9524 0.7246 1.8832 1.0730 1.6447 0.8403 0.6098 0.8547 0.2132 0.5618 0.4049 0.6452 1.1947 1.1111
MMAC-SF 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 1.1641 4.0000 0.7042 0.2315 0.4367 0.6494 1.0341 0.6897 1.5314 1.0764 1.6447 0.7813 0.5618 0.9346 0.2101 0.6289 0.4464 0.6173 1.1710 1.0406
MMAC-SF 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 1.0582 2.5063 0.5236 0.1919 0.2770 0.5319 0.7246 0.6211 1.2034 0.9346 1.1947 0.7576 0.5291 0.7519 0.1736 0.5882 0.3817 0.3623 1.0593 1.0000
MMAC-SF 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 1.1507 2.8090 0.5587 0.2041 0.3106 0.6536 0.8696 0.6024 1.3316 1.0081 1.2195 0.6329 0.5814 0.7752 0.1842 0.5814 0.3413 0.4425 1.0823 0.9709
MMAC-SF 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 1.2376 4.7393 0.6061 0.2451 0.4237 1.0152 0.7692 1.0060 1.5748 0.8197 0.4808 0.8850 0.2123 0.6135 0.4484 0.6250 1.0395 1.2195
MMAC-SF 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 1.1962 3.8023 0.6211 0.2058 0.3534 0.5917 0.9259 0.5208 1.3569 0.9804 1.6920 0.7353 0.4505 0.8475 0.1980 0.4546 0.6667 0.9804 0.9804
MMAC-SF 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 1.2361 3.8462 0.6369 0.2119 0.3774 0.5747 0.9174 0.7299 1.4327 0.8197 1.4881 0.7353 0.5348 0.7937 0.1818 0.5714 0.4425 0.5263 1.0417 1.0471
MMAC-SF 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 1.2563 4.0161 0.6667 0.1965 0.4292 0.6250 1.0267 0.5952 1.5337 0.8850 1.4706 0.8475 0.5650 0.8772 0.2105 0.5780 0.4098 0.6173 1.2005 1.1274
MMAC-SF 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 1.2034 3.6232 0.6494 0.2232 0.3891 0.6024 0.9434 0.7194 1.4556 0.9009 1.4793 0.8130 0.5102 0.7813 0.2110 0.5747 0.4525 0.5405 1.1261 1.0560
MMAC-SF 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 1.1614 3.4965 0.6536 0.1992 0.4237 0.5618 1.0526 0.5882 1.3106 0.9259 1.4409 0.7937 0.4951 0.7937 0.2037 0.5882 0.4405 0.5587 1.2005 1.0352
MMAC-SF 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.5952 0.1825 0.2933 0.5495 0.8000 0.6173 1.1442 0.8929 1.3263 0.7143 0.4878 0.8547 0.1802 0.6173 0.4032 0.4292 1.1123 1.0449
MMAC-SF 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 1.2610 3.2787 0.6757 0.1953 0.3861 0.6410 1.0471 0.6211 1.4599 0.9524 1.2674 0.8772 0.5814 0.7634 0.2128 0.5780 0.3677 0.5025 1.2723 1.1099
MMAC-SF 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 1.1601 3.7175 0.5650 0.2212 0.3356 0.6944 0.9009 0.7143 1.5798 0.9434 1.3038 0.7576 0.5780 0.7874 0.1980 0.5682 0.4202 0.4566 0.9804
MMAC-SF 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 1.2453 3.2258 0.5917 0.2222 0.3436 0.6329 0.8929 0.6211 1.3812 0.9259 1.2771 0.7299 0.5405 0.8850 0.6579 0.4132 0.4902 1.0341 1.0309
MMAC-SF 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 1.2063 3.2258 0.5848 0.3155 0.5988 0.7874 0.6623 1.4388 0.9434 1.2210 0.7576 0.5319 0.8403 0.1965 0.5917 0.4255 0.4464 1.0549 1.0288
MMAC-SF 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 1.3263 3.3113 0.6135 0.2257 0.3937 0.6173 0.9901 0.7353 1.2804 0.9346 1.3495 0.8265 0.5714 0.7752 0.1923 0.5714 0.4367 0.4673 1.0965 1.0081
MMAC-SF 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 1.1351 3.1746 0.5525 0.2049 0.3413 0.6211 0.8475 0.6494 1.3459 0.8696 1.2180 0.8197 0.5155 0.7937 0.2012 0.6098 0.4082 0.4902 0.9346 1.0215
MMAC-SF 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 1.2121 3.0488 0.5988 0.2119 0.3891 0.6061 0.9174 0.5917 1.2674 0.8850 1.2484 0.8197 0.5236 0.8197 0.1949 0.6024 0.3984 0.5051 1.0299 1.0299
MMAC-SF 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 1.1325 2.2936 0.5435 0.1988 0.2611 0.6211 0.6289 1.0764 0.7937 1.0352 0.6711 0.5181 0.7042 0.1953 0.5988 0.3546 0.3115 1.0215 1.0163
MMAC-SF 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 1.2225 2.3981 0.5650 0.2165 0.2941 0.6623 0.7813 0.6757 1.0549 0.9259 1.1236 0.7042 0.6024 0.7576 0.1957 0.6211 0.3584 0.3413 1.0870 1.0858
MMAC-SF 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 1.0471 2.3529 0.4785 0.2132 0.2506 0.5682 0.6369 0.6173 1.1862 0.8475 1.0504 0.6897 0.5435 0.7407 0.1818 0.5525 0.3559 0.3322 0.8929 0.8000
MMAC-SF 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 1.0288 2.1930 0.5025 0.2179 0.2865 0.5465 0.7246 0.6061 1.1364 0.8403 1.0215 0.7194 0.5102 0.6897 0.1848 0.5780 0.3205 0.9804 0.8696
MMAC-SF 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.6410 1.5552 0.3534 0.2114 0.3953 0.5025 0.4049 0.9346 0.6579 0.6211 0.5376 0.3953 0.5155 0.1961 0.5814 0.3546 0.2632 0.7092 0.7143
MMAC-SF 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.6944 1.5361 0.3497 0.2066 0.2347 0.4425 0.5587 0.4274 1.0000 0.6579 0.6410 0.5155 0.3891 0.4926 0.1905 0.5650 0.3390 0.2639 0.7299 0.6024