Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.0991 0.7752 0.0120 0.0119 0.0130 0.1618 0.0319 0.2801 0.0312 0.0354 0.3345 0.0651 0.0111 0.0591 0.0897 0.0225
WM1552C 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.0914 0.6494 0.0113 0.0114 0.0114 0.1399 0.0285 0.2597 0.0245 0.0283 0.2732 0.0476 0.0624 0.0079 0.0519 0.0144 0.0754 0.0344
WM1552C 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.0959 0.6757 0.0131 0.0116 0.0176 0.1484 0.0325 0.2778 0.0256 0.0331 0.3003 0.0468 0.0687 0.0095 0.0520 0.0130 0.0789 0.0230
WM1552C 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.0907 0.7092 0.0111 0.0118 0.0171 0.1789 0.0235 0.2353 0.0180 0.0325 0.3185 0.0359 0.0587 0.0091 0.0584 0.0121 0.0539 0.0370
WM1552C 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.0979 0.7519 0.0132 0.0119 0.0104 0.1739 0.0250 0.2439 0.0155 0.0341 0.3413 0.0350 0.0685 0.0101 0.0487 0.0143 0.0565 0.0217
WM1552C 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
WM1552C 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
WM1552C 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.0941 0.8130 0.0151 0.0119 0.0143 0.2994 0.0471 0.2899 0.0415 0.0429 0.5495 0.0837 0.0761 0.0095 0.1876 0.0404 0.1330 0.0333
WM1552C 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.1066 0.8265 0.0148 0.0131 0.0123 0.2653 0.0518 0.2494 0.0366 0.0441 0.5814 0.0962 0.0874 0.0116 0.1776 0.0352 0.1548 0.0256
WM1552C 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.0948 0.8403 0.0149 0.0118 0.0141 0.2545 0.0438 0.2667 0.0353 0.0352 0.4762 0.0803 0.0751 0.0101 0.1550 0.0349 0.1239 0.0296
WM1552C 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.0974 0.8547 0.0151 0.0131 0.2571 0.0493 0.2801 0.0385 0.0457 0.5102 0.0680 0.0769 0.0128 0.1307 0.0310 0.1250 0.0259
WM1552C 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.0953 0.7519 0.0133 0.0121 0.0132 0.2151 0.0406 0.2674 0.0333 0.0368 0.3891 0.0660 0.0726 0.0096 0.0990 0.0248 0.0931 0.0369
WM1552C 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.1006 0.7813 0.0144 0.0120 0.0204 0.2049 0.0386 0.2667 0.0348 0.0397 0.4132 0.0677 0.0792 0.0099 0.0810 0.0208 0.1010 0.0328
WM1552C 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.0865 0.7194 0.0114 0.0134 0.1642 0.0230 0.2451 0.0172 0.0319 0.3236 0.0372 0.0604 0.0093 0.0595 0.0149 0.0531 0.0277
WM1552C 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.0928 0.7519 0.0138 0.0104 0.0157 0.1923 0.0228 0.2933 0.0170 0.0412 0.3571 0.0420 0.0650 0.0106 0.0464 0.0174 0.0461 0.0215
WM1552C 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.0948 0.7407 0.0120 0.0106 0.0139 0.2304 0.0403 0.2519 0.0354 0.0373 0.4255 0.0647 0.0745 0.0097 0.1029 0.0221 0.0983 0.0287
WM1552C 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.1030 0.7752 0.0127 0.0122 0.0202 0.2020 0.0432 0.2273 0.0337 0.0387 0.4405 0.0610 0.0805 0.0102 0.1058 0.0227 0.1192 0.0256
WM1552C 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.0895 0.7299 0.0110 0.0108 0.0136 0.1748 0.0380 0.2336 0.0323 0.0350 0.3534 0.0596 0.0729 0.0094 0.0842 0.0195 0.0978 0.0288
WM1552C 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.0968 0.7194 0.0128 0.0104 0.0199 0.1992 0.0403 0.2538 0.0338 0.0386 0.3718 0.0543 0.0698 0.0102 0.0953 0.0206 0.1009 0.0283
WM1552C 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.0903 0.7692 0.0144 0.0114 0.0138 0.2262 0.0410 0.2564 0.0347 0.0375 0.4651 0.0732 0.0782 0.0099 0.1272 0.0252 0.1112 0.0273
WM1552C 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.1026 0.7634 0.0133 0.0126 0.0199 0.2416 0.0442 0.2564 0.0321 0.0414 0.4975 0.0671 0.0803 0.0115 0.1368 0.0295 0.1193 0.0270
WM1552C 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.0858 0.7692 0.0109 0.0104 0.0129 0.1890 0.0318 0.2242 0.0334 0.3846 0.0581 0.0674 0.0101 0.0814 0.0169 0.0899 0.0230
WM1552C 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.0930 0.7463 0.0149 0.0124 0.0151 0.2273 0.0362 0.0317 0.0425 0.4115 0.0587 0.0635 0.0116 0.0847 0.0242 0.0868 0.0251
WM1552C 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.0940 0.7519 0.0137 0.0103 0.0206 0.2421 0.0422 0.2874 0.0311 0.0400 0.4831 0.0743 0.0833 0.0094 0.1198 0.0235 0.0944 0.0328
WM1552C 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.1007 0.7353 0.0139 0.0120 0.0180 0.2247 0.0444 0.2551 0.0375 0.0392 0.4630 0.0684 0.0836 0.0099 0.1191 0.0306 0.1094 0.0273