Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

 Full screen view
Filter results: >>>>
Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.0912 0.7813 0.0126 0.0112 0.0119 0.1984 0.0389 0.2364 0.0310 0.0369 0.3984 0.0673 0.0763 0.0094 0.0889 0.0178 0.1018 0.0293
WM1552C 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.1006 0.7299 0.0152 0.0110 0.0191 0.2212 0.0345 0.2571 0.0334 0.0452 0.3846 0.0635 0.0734 0.0101 0.1002 0.0210 0.0946 0.0296
WM1552C 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.0945 0.8265 0.0149 0.0126 0.0198 0.2451 0.0407 0.2674 0.0387 0.0442 0.4546 0.0721 0.0824 0.0101 0.1534 0.0305 0.1072 0.0322
WM1552C 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.1058 0.7692 0.0149 0.0131 0.0208 0.2451 0.0342 0.2801 0.0338 0.0442 0.4587 0.0612 0.0812 0.0106 0.1134 0.0299 0.1116 0.0303
WM1552C 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.7519 0.0114 0.0122 0.0123 0.1822 0.0337 0.2268 0.0272 0.0384 0.3745 0.0555 0.0736 0.0112 0.0772 0.0172 0.0973 0.0255
WM1552C 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.0985 0.7752 0.0157 0.0134 0.0144 0.2310 0.0357 0.2959 0.0282 0.0456 0.3817 0.0606 0.0123 0.0791 0.0212 0.0942 0.0303
WM1552C 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.0969 0.6897 0.0133 0.0106 0.0131 0.2203 0.0453 0.2242 0.0458 0.0377 0.4405 0.0677 0.0702 0.0088 0.1095 0.0203 0.1182 0.0296
WM1552C 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.1031 0.7752 0.0142 0.0124 0.0157 0.2016 0.0323 0.2247 0.0404 0.0374 0.4546 0.0689 0.0933 0.0114 0.1168 0.0248 0.1221 0.0215
WM1552C 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.0982 0.6623 0.0122 0.0121 0.0177 0.1754 0.0362 0.2469 0.0340 0.0340 0.3175 0.0615 0.0673 0.0097 0.0775 0.0171 0.0916 0.0437
WM1552C 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.1035 0.7246 0.0129 0.0124 0.0192 0.1862 0.0394 0.2717 0.0350 0.0359 0.3663 0.0539 0.0748 0.0103 0.0711 0.0164 0.0959 0.0263
WM1552C 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.1010 0.7299 0.0130 0.0102 0.0129 0.2049 0.0404 0.2571 0.0426 0.0340 0.4184 0.0732 0.0734 0.0102 0.1026 0.0191 0.1112 0.0304
WM1552C 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.1100 0.8065 0.0131 0.0130 0.0127 0.2232 0.0465 0.2907 0.0376 0.0383 0.4608 0.0613 0.0856 0.0113 0.1005 0.0260 0.1148 0.0239
WM1552C 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.0946 0.7092 0.0110 0.0116 0.0107 0.1613 0.0277 0.2331 0.0300 0.0301 0.3195 0.0539 0.0641 0.0082 0.0618 0.0200 0.0794 0.0378
WM1552C 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.0965 0.7874 0.0145 0.0123 0.0185 0.1927 0.0297 0.2747 0.0309 0.0358 0.3597 0.0477 0.0736 0.0104 0.0554 0.0178 0.0791 0.0279
WM1552C 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.0916 0.6536 0.0126 0.0105 0.0132 0.2310 0.0424 0.2463 0.0423 0.0359 0.4464 0.0636 0.0729 0.0093 0.1086 0.0214 0.1120 0.0280
WM1552C 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.0988 0.7194 0.0117 0.0123 0.0132 0.2020 0.0420 0.1934 0.0390 0.0358 0.4566 0.0619 0.0858 0.0120 0.1062 0.0263 0.1267 0.0217
WM1552C 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.0930 0.6711 0.0114 0.0113 0.0129 0.1799 0.0375 0.2571 0.0361 0.0332 0.3677 0.0646 0.0688 0.0094 0.0835 0.0283 0.1104 0.0278
WM1552C 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.0995 0.7407 0.0128 0.0129 0.0183 0.1894 0.0401 0.2584 0.0345 0.0378 0.3953 0.0571 0.0767 0.0104 0.0911 0.0225 0.1185 0.0258
WM1552C 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.0949 0.6711 0.0135 0.0103 0.0122 0.2227 0.0374 0.2110 0.0368 0.0365 0.4065 0.0643 0.0739 0.0101 0.1171 0.0271 0.0970 0.0357
WM1552C 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.1000 0.7143 0.0148 0.0116 0.0201 0.2079 0.0399 0.1988 0.0347 0.0376 0.4630 0.0589 0.0751 0.0105 0.0863 0.0209 0.1107 0.0282
WM1552C 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.0882 0.6757 0.0114 0.0124 0.0125 0.1862 0.0300 0.2262 0.0276 0.3521 0.0531 0.0634 0.0092 0.0680 0.0217 0.0835 0.0341
WM1552C 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.0902 0.7299 0.0134 0.0110 0.0211 0.2033 0.0317 0.2433 0.0307 0.0369 0.3922 0.0474 0.0668 0.0107 0.0632 0.0193 0.0818 0.0303
WM1552C 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
WM1552C 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
WM1552C 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.0872 0.6623 0.0112 0.0098 0.0114 0.1513 0.0307 0.2387 0.0315 0.0315 0.2994 0.0518 0.0664 0.0086 0.0678 0.0167 0.0859 0.0285