Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0242 0.5376 0.0820 0.0337 0.0460 0.0149 0.0071 0.0089 0.0099 0.0227 0.0502 0.0355 0.1112 0.0215 0.0085 0.0066 0.0123 0.0180 0.0085 0.0876 0.0800
WM1552C 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0336 0.6211 0.0915 0.0377 0.0582 0.0166 0.0071 0.0092 0.0094 0.0233 0.0640 0.0390 0.1681 0.0274 0.0057 0.0064 0.0102 0.0226 0.0088 0.1193 0.1015
WM1552C 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0199 0.2488 0.0350 0.0173 0.0083 0.0068 0.0039 0.0043 0.0046 0.0261 0.0209 0.0184 0.0541 0.0113 0.0050 0.0037 0.0068 0.0078 0.0051 0.0678 0.0543
WM1552C 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0226 0.3268 0.0409 0.0176 0.0087 0.0074 0.0032 0.0052 0.0046 0.0250 0.0285 0.0204 0.0773 0.0099 0.0044 0.0032 0.0067 0.0074 0.0043 0.0794 0.0643
WM1552C 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0337 0.7576 0.0877 0.0460 0.0364 0.0270 0.0099 0.0145 0.0156 0.0292 0.1015 0.0570 0.1669 0.0339 0.0154 0.0098 0.0200 0.0273 0.0134 0.1506 0.1087
WM1552C 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0451 0.9709 0.1181 0.0523 0.0448 0.0264 0.0069 0.0154 0.0150 0.0310 0.1339 0.0696 0.2500 0.0323 0.0126 0.0068 0.0132 0.0309 0.0169 0.1923 0.1377
WM1552C 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0214 0.2976 0.0464 0.0228 0.0175 0.0106 0.0045 0.0057 0.0061 0.0185 0.0417 0.0239 0.0717 0.0124 0.0062 0.0042 0.0076 0.0088 0.0054 0.0739 0.0649
WM1552C 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0235 0.3472 0.0869 0.0232 0.0208 0.0122 0.0043 0.0059 0.0061 0.0170 0.0547 0.0281 0.0978 0.0116 0.0046 0.0037 0.0067 0.0097 0.0048 0.0805 0.0735
WM1552C 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0297 0.6667 0.0785 0.0361 0.0164 0.0161 0.0082 0.0117 0.0121 0.0218 0.0625 0.0514 0.1282 0.0269 0.0119 0.0078 0.0128 0.0204 0.0088 0.1212 0.0883
WM1552C 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0335 0.8475 0.0778 0.0365 0.0177 0.0168 0.0076 0.0101 0.0086 0.0230 0.0646 0.0529 0.1721 0.0258 0.0112 0.0054 0.0132 0.0194 0.0104 0.1318 0.0996
WM1552C 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0178 0.2066 0.0306 0.0132 0.0043 0.0051 0.0030 0.0041 0.0037 0.0186 0.0188 0.0163 0.0415 0.0078 0.0039 0.0032 0.0048 0.0047 0.0035 0.0505 0.0411
WM1552C 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0209 0.2392 0.0341 0.0144 0.0047 0.0068 0.0031 0.0029 0.0040 0.0268 0.0237 0.0171 0.0588 0.0080 0.0036 0.0026 0.0050 0.0051 0.0034 0.0575 0.0641
WM1552C 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0307 0.6211 0.0934 0.0382 0.0438 0.0226 0.0074 0.0115 0.0115 0.0259 0.0943 0.0570 0.1647 0.0287 0.0108 0.0077 0.0125 0.0177 0.0096 0.1758 0.0931
WM1552C 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0360 0.8475 0.1047 0.0450 0.0516 0.0244 0.0080 0.0069 0.0108 0.0306 0.1101 0.0601 0.2155 0.0281 0.0123 0.0043 0.0171 0.0252 0.0105 0.1916 0.1276
WM1552C 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0181 0.2519 0.0421 0.0189 0.0146 0.0090 0.0036 0.0051 0.0042 0.0158 0.0396 0.0171 0.0608 0.0112 0.0043 0.0037 0.0058 0.0070 0.0040 0.0787 0.0435
WM1552C 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0235 0.3165 0.0525 0.0229 0.0256 0.0131 0.0042 0.0042 0.0058 0.0151 0.0596 0.0201 0.0923 0.0117 0.0047 0.0031 0.0076 0.0110 0.0041 0.0880 0.0714
WM1552C 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0224 0.4587 0.0637 0.0279 0.0098 0.0119 0.0058 0.0077 0.0078 0.0199 0.0478 0.0316 0.0906 0.0191 0.0072 0.0058 0.0085 0.0143 0.0064 0.1022 0.0656
WM1552C 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0275 0.5155 0.0555 0.0315 0.0088 0.0128 0.0046 0.0057 0.0068 0.0204 0.0543 0.0316 0.1201 0.0207 0.0071 0.0053 0.0096 0.0104 0.0066 0.1070 0.0950
WM1552C 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0178 0.2257 0.0314 0.0144 0.0053 0.0054 0.0028 0.0041 0.0041 0.0231 0.0209 0.0166 0.0450 0.0085 0.0046 0.0037 0.0054 0.0057 0.0041 0.0647 0.0452
WM1552C 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0233 0.2725 0.0360 0.0181 0.0056 0.0085 0.0032 0.0033 0.0045 0.0237 0.0250 0.0177 0.0668 0.0088 0.0042 0.0032 0.0067 0.0068 0.0046 0.0745 0.0737
WM1552C 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0218 0.4255 0.0717 0.0305 0.0263 0.0153 0.0054 0.0085 0.0078 0.0250 0.0633 0.0366 0.0925 0.0172 0.0072 0.0061 0.0098 0.0138 0.0071 0.1046 0.0636
WM1552C 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0315 0.5236 0.0750 0.0409 0.0284 0.0194 0.0057 0.0081 0.0085 0.0270 0.0688 0.0364 0.1271 0.0200 0.0083 0.0043 0.0109 0.0149 0.0074 0.1168 0.0943
WM1552C 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0186 0.2053 0.0295 0.0146 0.0100 0.0076 0.0029 0.0038 0.0039 0.0267 0.0171 0.0483 0.0089 0.0048 0.0040 0.0053 0.0049 0.0044 0.0709 0.0466
WM1552C 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0258 0.2463 0.0370 0.0180 0.0135 0.0116 0.0036 0.0047 0.0056 0.0273 0.0366 0.0192 0.0736 0.0097 0.0051 0.0041 0.0053 0.0085 0.0046 0.0766 0.0739
WM1552C 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0188 0.1934 0.0226 0.0110 0.0040 0.0053 0.0030 0.0038 0.0041 0.0692 0.0159 0.0159 0.0452 0.0093 0.0044 0.0035 0.0052 0.0051 0.0047 0.0647 0.0542