Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0234 0.3012 0.0453 0.0198 0.0065 0.0100 0.0042 0.0059 0.0061 0.0180 0.0466 0.0259 0.0895 0.0112 0.0049 0.0038 0.0065 0.0091 0.0048 0.0749 0.0706
WM1552C 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0294 0.5587 0.0690 0.0365 0.0123 0.0147 0.0066 0.0096 0.0092 0.0258 0.0543 0.0400 0.1229 0.0246 0.0089 0.0069 0.0112 0.0129 0.0082 0.1112 0.0769
WM1552C 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0322 0.7634 0.0716 0.0433 0.0133 0.0163 0.0066 0.0097 0.0094 0.0286 0.0612 0.0414 0.1618 0.0244 0.0057 0.0045 0.0139 0.0150 0.0085 0.1264 0.0942
WM1552C 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0175 0.1957 0.0284 0.0130 0.0073 0.0045 0.0027 0.0040 0.0036 0.0361 0.0168 0.0151 0.0416 0.0078 0.0039 0.0030 0.0049 0.0039 0.0036 0.0587 0.0366
WM1552C 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0209 0.2320 0.0333 0.0155 0.0087 0.0063 0.0031 0.0034 0.0043 0.0342 0.0216 0.0164 0.0668 0.0084 0.0039 0.0026 0.0060 0.0052 0.0035 0.0661 0.0620
WM1552C 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0290 0.6098 0.0709 0.0392 0.0149 0.0195 0.0085 0.0122 0.0110 0.0321 0.0912 0.0465 0.1416 0.0263 0.0104 0.0083 0.0125 0.0262 0.0099 0.1235 0.0864
WM1552C 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0370 0.8403 0.0866 0.0510 0.0164 0.0236 0.0076 0.0074 0.0118 0.0357 0.1114 0.0520 0.1988 0.0240 0.0072 0.0046 0.0168 0.0149 0.0107 0.1401 0.1319
WM1552C 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0188 0.2252 0.0328 0.0180 0.0070 0.0074 0.0031 0.0046 0.0041 0.0234 0.0305 0.0184 0.0491 0.0091 0.0046 0.0036 0.0051 0.0046 0.0041 0.0574 0.0425
WM1552C 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0244 0.2933 0.0425 0.0209 0.0109 0.0109 0.0031 0.0049 0.0065 0.0218 0.0450 0.0241 0.0759 0.0103 0.0061 0.0036 0.0068 0.0096 0.0045 0.0623 0.0717
WM1552C 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0336 0.7937 0.0774 0.0353 0.0204 0.0161 0.0073 0.0118 0.0130 0.0315 0.0658 0.0585 0.1346 0.0312 0.0115 0.0082 0.0124 0.0211 0.0113 0.1707 0.1011
WM1552C 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0412 1.0417 0.0916 0.0513 0.0223 0.0191 0.0073 0.0100 0.0131 0.0326 0.0769 0.0464 0.1751 0.0298 0.0108 0.0057 0.0140 0.0220 0.0119 0.1783 0.1421
WM1552C 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0286 0.5435 0.0751 0.0318 0.0120 0.0107 0.0055 0.0079 0.0070 0.0191 0.0468 0.0392 0.0977 0.0218 0.0076 0.0069 0.0107 0.0104 0.0072 0.1282 0.0841
WM1552C 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0417 0.7143 0.0861 0.0462 0.0163 0.0192 0.0074 0.0074 0.0104 0.0213 0.0656 0.0434 0.1575 0.0228 0.0080 0.0064 0.0137 0.0216 0.0075 0.1518 0.1506
WM1552C 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0296 0.6897 0.0823 0.0385 0.0142 0.0182 0.0072 0.0123 0.0124 0.0342 0.0879 0.0526 0.1379 0.0301 0.0109 0.0093 0.0146 0.0173 0.0109 0.1825 0.0999
WM1552C 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0416 0.8850 0.0880 0.0543 0.0173 0.0275 0.0096 0.0133 0.0142 0.0335 0.1082 0.0555 0.1880 0.0313 0.0100 0.0072 0.0179 0.0283 0.0123 0.2020 0.1511
WM1552C 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0206 0.2874 0.0349 0.0177 0.0095 0.0093 0.0034 0.0046 0.0048 0.0238 0.0400 0.0214 0.0644 0.0121 0.0057 0.0043 0.0063 0.0067 0.0049 0.0819 0.0577
WM1552C 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0307 0.4000 0.0527 0.0244 0.0137 0.0148 0.0050 0.0072 0.0077 0.0221 0.0633 0.0284 0.1109 0.0160 0.0073 0.0066 0.0073 0.0140 0.0058 0.0962 0.0929
WM1552C 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0186 0.2725 0.0410 0.0185 0.0143 0.0070 0.0041 0.0056 0.0048 0.0482 0.0205 0.0208 0.0586 0.0123 0.0050 0.0044 0.0067 0.0090 0.0048 0.0577 0.0428
WM1552C 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0256 0.3021 0.0402 0.0202 0.0136 0.0084 0.0038 0.0043 0.0047 0.0551 0.0243 0.0180 0.0733 0.0138 0.0049 0.0033 0.0073 0.0085 0.0049 0.0611 0.0589
WM1552C 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0209 0.2237 0.0343 0.0154 0.0126 0.0059 0.0031 0.0042 0.0044 0.0775 0.0176 0.0176 0.0463 0.0096 0.0049 0.0035 0.0058 0.0055 0.0043 0.0581 0.0501
WM1552C 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0264 0.2710 0.0359 0.0161 0.0137 0.0070 0.0031 0.0043 0.0043 0.0551 0.0219 0.0177 0.0690 0.0110 0.0040 0.0027 0.0060 0.0068 0.0045 0.0632 0.0696
WM1552C 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0351 0.8000 0.1100 0.0485 0.0518 0.0283 0.0093 0.0134 0.0141 0.0418 0.1013 0.0594 0.1435 0.0367 0.0128 0.0178 0.0209 0.0121 0.1669 0.1229
WM1552C 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.9524 0.1121 0.0620 0.0527 0.0299 0.0096 0.0139 0.0149 0.0422 0.1218 0.0603 0.0368 0.0134 0.0095 0.0174 0.0134 0.1883 0.1650
WM1552C 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0244 0.2994 0.0469 0.0252 0.0122 0.0118 0.0038 0.0054 0.0072 0.0320 0.0421 0.0230 0.0663 0.0135 0.0061 0.0040 0.0072 0.0079 0.0053 0.0636 0.0591
WM1552C 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0282 0.3534 0.0614 0.0300 0.0158 0.0148 0.0033 0.0049 0.0073 0.0238 0.0590 0.0274 0.0990 0.0138 0.0058 0.0039 0.0075 0.0133 0.0052 0.0732 0.0852