Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.0768 0.5814 0.0126 0.0233 0.2114 0.0352 0.2421 0.0388 0.0359 0.4032 0.0360 0.0280 0.0080 0.0833 0.0162 0.0494 0.0394
WM1552C 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.0807 0.5882 0.0114 0.0098 0.0219 0.1953 0.0359 0.0369 0.0311 0.4132 0.0318 0.0334 0.0091 0.0663 0.0169 0.0583 0.0290
WM1552C 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.0811 0.6061 0.0116 0.0104 0.0210 0.1742 0.0306 0.2421 0.0348 0.0316 0.3460 0.0305 0.0299 0.0078 0.0762 0.0174 0.0459 0.0413
WM1552C 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.0840 0.6211 0.0129 0.0104 0.0281 0.1799 0.0341 0.2421 0.0318 0.0332 0.3745 0.0267 0.0329 0.0083 0.0665 0.0172 0.0513 0.0358
WM1552C 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.0794 0.5128 0.0124 0.0100 0.0333 0.2247 0.0340 0.2410 0.0536 0.0335 0.4630 0.0500 0.0269 0.0087 0.1174 0.0265 0.0626 0.0296
WM1552C 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.0880 0.5465 0.0128 0.0098 0.0342 0.2151 0.0376 0.2288 0.0504 0.0335 0.4878 0.0448 0.0305 0.0086 0.1160 0.0264 0.0678 0.0264
WM1552C 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.0775 0.4926 0.0100 0.0098 0.0253 0.1733 0.0262 0.2160 0.0407 0.0282 0.3484 0.0292 0.0266 0.0075 0.0711 0.0198 0.0447 0.0309
WM1552C 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.0817 0.5236 0.0119 0.0094 0.0330 0.1946 0.0282 0.2288 0.0418 0.0339 0.3774 0.0246 0.0297 0.0084 0.0600 0.0192 0.0444 0.0299
WM1552C 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.0702 0.5556 0.0115 0.0087 0.0226 0.1988 0.0305 0.2320 0.0346 0.0327 0.3891 0.0343 0.0275 0.0082 0.0930 0.0178 0.0557 0.0288
WM1552C 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.0816 0.5952 0.0110 0.0109 0.0270 0.1767 0.0345 0.2016 0.0353 0.0315 0.4049 0.0312 0.0303 0.0079 0.0787 0.0208 0.0666 0.0247
WM1552C 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.0769 0.5682 0.0116 0.0086 0.0234 0.1669 0.0298 0.2283 0.0316 0.0302 0.3401 0.0328 0.0299 0.0078 0.0742 0.0176 0.0537 0.0290
WM1552C 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.0806 0.5882 0.0119 0.0095 0.0292 0.1832 0.0315 0.2398 0.0304 0.0331 0.3636 0.0282 0.0290 0.0085 0.0836 0.0186 0.0568 0.0271
WM1552C 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.0775 0.4926 0.0131 0.0091 0.0266 0.2062 0.0301 0.2096 0.0457 0.0349 0.4237 0.0411 0.0264 0.0078 0.1116 0.0213 0.0492 0.0253
WM1552C 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.0842 0.5102 0.0125 0.0098 0.0324 0.2062 0.0333 0.2132 0.0449 0.0332 0.4484 0.0392 0.0307 0.0083 0.1015 0.0223 0.0557 0.0236
WM1552C 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.0727 0.4525 0.0087 0.0227 0.1524 0.0238 0.1855 0.0287 0.0276 0.3155 0.0274 0.0251 0.0073 0.0573 0.0115 0.0408 0.0269
WM1552C 0.0000
uM
 1
hr
 7
Odyssey/Microvigene
 0.0786 0.4651 0.0123 0.0085 0.0285 0.1695 0.0263 0.2198 0.0313 0.0324 0.3195 0.0248 0.0255 0.0092 0.0572 0.0159 0.0408 0.0290
WM1552C 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.0729 0.5465 0.0119 0.0094 0.0253 0.1715 0.0272 0.2421 0.0297 0.0310 0.3460 0.0283 0.0287 0.0072 0.0582 0.0127 0.0392 0.0299
WM1552C 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.0789 0.5650 0.0123 0.0099 0.0237 0.1600 0.0238 0.2304 0.0286 0.0286 0.3390 0.0288 0.0288 0.0084 0.0675 0.0140 0.0513 0.0231
WM1552C 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.0780 0.5747 0.0114 0.0088 0.0192 0.1592 0.0259 0.2288 0.0258 0.0278 0.3086 0.0277 0.0299 0.0079 0.0668 0.0144 0.0471 0.0236
WM1552C 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.0832 0.5435 0.0119 0.0090 0.0262 0.1707 0.0220 0.2262 0.0272 0.0309 0.3236 0.0262 0.0291 0.0088 0.0709 0.0157 0.0449 0.0242
WM1552C 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.0695 0.4484 0.0102 0.0081 0.0280 0.1355 0.0202 0.2114 0.0270 0.0295 0.2778 0.0234 0.0238 0.0072 0.0507 0.0104 0.0307 0.0301
WM1552C 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.0765 0.4505 0.0110 0.0097 0.0213 0.1409 0.0171 0.0249 0.0303 0.2674 0.0209 0.0252 0.0083 0.0452 0.0108 0.0339 0.0243
WM1552C 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.0737 0.4425 0.0112 0.0084 0.0259 0.1054 0.0183 0.2070 0.0219 0.0266 0.2494 0.0173 0.0266 0.0078 0.0420 0.0110 0.0281 0.0245
WM1552C 0.0000
uM
 1
hr
 8
Odyssey/Microvigene
 0.0788 0.4608 0.0102 0.0087 0.0228 0.1445 0.0164 0.2353 0.0214 0.0309 0.2513 0.0181 0.0206 0.0079 0.0384 0.0124 0.0266
WM1552C 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.0928 0.6897 0.0110 0.0142 0.1681 0.0316 0.2404 0.0328 0.0310 0.3413 0.0562 0.0626 0.0083 0.0629 0.0140 0.0818 0.0365