Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.1395 0.3906 0.0119 0.0073 0.0096 0.0150 0.0227 0.1880 0.0135 0.0983 0.0133 0.0054 0.0058 0.0180 0.0041 0.0411 0.0132 0.0129 0.1401 0.0407
RVH-421 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.1605 0.3788 0.0123 0.0100 0.0089 0.0191 0.0264 0.1795 0.0151 0.1010 0.0156 0.0054 0.0052 0.0177 0.0063 0.0523 0.0144 0.0112 0.1595 0.0424
RVH-421 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.2123 0.4762 0.0134 0.0121 0.0101 0.0149 0.0284 0.1873 0.0184 0.0903 0.0198 0.0066 0.0050 0.0153 0.0244 0.0165 0.0092 0.1070 0.0592
RVH-421 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.2268 0.4739 0.0135 0.0140 0.0081 0.0193 0.0282 0.2278 0.0175 0.0908 0.0255 0.0057 0.0049 0.0148 0.0106 0.0306 0.0168 0.0086 0.1201 0.0482
RVH-421 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.1838 0.2571 0.0082 0.0062 0.0079 0.0119 0.0203 0.2141 0.0117 0.1035 0.0187 0.0049 0.0043 0.0092 0.0040 0.0405 0.0101 0.0146 0.1721 0.0580
RVH-421 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.1439 0.2488 0.0108 0.0096 0.0079 0.0222 0.0205 0.2004 0.0163 0.0976 0.0140 0.0047 0.0054 0.0055 0.0055 0.0437 0.0115 0.0120 0.1919 0.0387
RVH-421 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.1905 0.3509 0.0140 0.0090 0.0090 0.0141 0.0200 0.1595 0.0144 0.0763 0.0188 0.0057 0.0039 0.0174 0.0055 0.0282 0.0136 0.0098 0.0767 0.0493
RVH-421 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.2053 0.3390 0.0081 0.0082 0.0073 0.0174 0.0186 0.1767 0.0132 0.1127 0.0213 0.0050 0.0036 0.0179 0.0070 0.0311 0.0110 0.0091 0.1302 0.0436
RVH-421 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.1337 0.4255 0.0086 0.0055 0.0085 0.0127 0.0151 0.1972 0.0125 0.0733 0.0110 0.0060 0.0047 0.0217 0.0041 0.0333 0.0100 0.0105 0.1122 0.0374
RVH-421 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.1751 0.4115 0.0079 0.0076 0.0067 0.0154 0.0164 0.2008 0.0114 0.0776 0.0123 0.0058 0.0051 0.0199 0.0076 0.0365 0.0090 0.0100 0.1175 0.0523
RVH-421 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.2183 0.4348 0.0120 0.0097 0.0096 0.0153 0.0257 0.1701 0.0180 0.0803 0.0282 0.0065 0.0043 0.0156 0.0064 0.0243 0.0148 0.0095 0.0868 0.0543
RVH-421 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.2268 0.4484 0.0103 0.0103 0.0091 0.0200 0.0263 0.1818 0.0173 0.1074 0.0239 0.0064 0.0046 0.0147 0.0084 0.0307 0.0154 0.0102 0.1361 0.0590
RVH-421 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.2625 0.4115 0.0068 0.0057 0.0075 0.0128 0.0157 0.1934 0.0113 0.0656 0.0160 0.0061 0.0047 0.0139 0.0057 0.0238 0.0080 0.0088 0.0989 0.0768
RVH-421 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.2959 0.4000 0.0093 0.0084 0.0076 0.0175 0.0172 0.1689 0.0129 0.0704 0.0158 0.0063 0.0054 0.0127 0.0080 0.0257 0.0105 0.0084 0.1034 0.0888
RVH-421 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.1227 0.4292 0.0083 0.0078 0.0073 0.0111 0.0119 0.1789 0.0102 0.0738 0.0114 0.0065 0.0045 0.0281 0.0036 0.0326 0.0104 0.0097 0.0341
RVH-421 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.1701 0.4608 0.0067 0.0059 0.0061 0.0141 0.0125 0.2387 0.0090 0.0999 0.0066 0.0056 0.0284 0.0042 0.0320 0.0085 0.0094 0.1010 0.0425
RVH-421 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.1957 0.4065 0.0059 0.0049 0.0063 0.0106 0.0112 0.2451 0.0083 0.0740 0.0121 0.0063 0.0059 0.0231 0.0057 0.0304 0.0085 0.0091 0.1117 0.0713
RVH-421 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.2083 0.4115 0.0066 0.0059 0.0056 0.0132 0.0129 0.2611 0.0110 0.0768 0.0050 0.0233 0.0067 0.0367 0.0089 0.0091 0.1115 0.1114
RVH-421 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.1280 0.4425 0.0144 0.0102 0.0089 0.0149 0.0213 0.1855 0.0180 0.0974 0.0115 0.0063 0.0047 0.0196 0.0037 0.0278 0.0141 0.0136 0.1064 0.0293
RVH-421 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.1580 0.4785 0.0119 0.0099 0.0083 0.0193 0.0215 0.2058 0.0238 0.1247 0.0109 0.0067 0.0047 0.0192 0.0049 0.0343 0.0141 0.0123 0.0310
RVH-421 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.2326 0.4149 0.0061 0.0049 0.0071 0.0135 0.0115 0.2717 0.0086 0.0763 0.0113 0.0067 0.0068 0.0158 0.0062 0.0235 0.0121 0.0098 0.1175 0.0680
RVH-421 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.2551 0.4608 0.0085 0.0073 0.0069 0.0186 0.0152 0.2525 0.0128 0.0836 0.0117 0.0068 0.0061 0.0148 0.0072 0.0311 0.0096 0.0108 0.1120 0.0570
RVH-421 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.2092 0.4975 0.0055 0.0052 0.0059 0.0091 0.0098 0.2237 0.0078 0.1294 0.0136 0.0064 0.0046 0.0333 0.0048 0.0391 0.0094 0.0112 0.1035 0.0621
RVH-421 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.2278 0.6369 0.0056 0.0042 0.0053 0.0109 0.0105 0.2273 0.0089 0.2257 0.0137 0.0075 0.0053 0.0423 0.0052 0.0432 0.0093 0.0128 0.1316 0.1131
RVH-421 0.0000
uM
 48
hr
 8
InnoScan/Mapix