Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.2183 0.4098 0.0101 0.0085 0.0075 0.0210 0.0199 0.1905 0.0140 0.1064 0.0230 0.0062 0.0044 0.0197 0.0086 0.0287 0.0146 0.0096 0.0983 0.0750
RVH-421 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.1536 0.3636 0.0088 0.0073 0.0085 0.0157 0.0209 0.1698 0.0171 0.0799 0.0172 0.0057 0.0045 0.0169 0.0046 0.0423 0.0144 0.0141 0.1168 0.0491
RVH-421 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.1764 0.3745 0.0127 0.0120 0.0093 0.0219 0.0264 0.1783 0.0164 0.0969 0.0175 0.0049 0.0043 0.0153 0.0076 0.0471 0.0184 0.0134 0.1395 0.0694
RVH-421 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.2353 0.4785 0.0099 0.0081 0.0090 0.0153 0.0246 0.1822 0.0180 0.0693 0.0217 0.0078 0.0053 0.0191 0.0066 0.0255 0.0149 0.0086 0.0775 0.0581
RVH-421 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.2342 0.4739 0.0126 0.0118 0.0086 0.0213 0.0264 0.1808 0.0159 0.0991 0.0233 0.0078 0.0048 0.0172 0.0099 0.0263 0.0177 0.0079 0.1126 0.0716
RVH-421 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.1307 0.3115 0.0051 0.0067 0.0133 0.0149 0.0107 0.0741 0.0119 0.0053 0.0041 0.0116 0.0037 0.0371 0.0118 0.1272 0.0447
RVH-421 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.1852 0.3115 0.0109 0.0095 0.0075 0.0190 0.0194 0.1873 0.0125 0.0846 0.0116 0.0054 0.0047 0.0113 0.0056 0.0403 0.0124 0.0109 0.1393 0.0509
RVH-421 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.2315 0.4739 0.0163 0.0103 0.0089 0.0142 0.0197 0.1707 0.0179 0.0600 0.0267 0.0085 0.0052 0.0235 0.0070 0.0321 0.0183 0.0098 0.0671 0.0565
RVH-421 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.2353 0.5155 0.0120 0.0098 0.0080 0.0201 0.0223 0.1718 0.0170 0.1000 0.0257 0.0079 0.0057 0.0263 0.0098 0.0315 0.0179 0.0105 0.1201 0.0578
RVH-421 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.1475 0.3205 0.0109 0.0076 0.0085 0.0153 0.0184 0.1701 0.0164 0.0615 0.0175 0.0052 0.0045 0.0159 0.0048 0.0379 0.0185 0.0108 0.1040 0.0522
RVH-421 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.1684 0.3817 0.0138 0.0122 0.0093 0.0212 0.0245 0.0256 0.0792 0.0160 0.0051 0.0042 0.0154 0.0067 0.0402 0.0174 0.0092 0.1253 0.0451
RVH-421 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.2463 0.4464 0.0135 0.0132 0.0101 0.0162 0.0260 0.1733 0.0245 0.0672 0.0301 0.0072 0.0055 0.0190 0.0081 0.0238 0.0206 0.0095 0.0826 0.0627
RVH-421 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.2747 0.4926 0.0184 0.0148 0.0092 0.0207 0.0328 0.1805 0.0986 0.0299 0.0075 0.0055 0.0183 0.0115 0.0264 0.0227 0.0095 0.1144 0.0684
RVH-421 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.1282 0.2857 0.0069 0.0055 0.0068 0.0122 0.0122 0.0099 0.0733 0.0108 0.0055 0.0044 0.0112 0.0037 0.0388 0.0112 0.0111 0.1490 0.0290
RVH-421 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.1621 0.3226 0.0118 0.0102 0.0076 0.0167 0.0178 0.1661 0.0149 0.0835 0.0108 0.0049 0.0050 0.0106 0.0055 0.0471 0.0131 0.0105 0.1482 0.0478
RVH-421 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.2128 0.4098 0.0111 0.0097 0.0098 0.0144 0.0236 0.1585 0.0198 0.0644 0.0267 0.0085 0.0070 0.0256 0.0069 0.0366 0.0230 0.0101 0.0769 0.0541
RVH-421 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.2294 0.4878 0.0136 0.0098 0.0075 0.0198 0.0242 0.1531 0.0263 0.1418 0.0244 0.0075 0.0065 0.0302 0.0093 0.0325 0.0182 0.0107 0.1040 0.0600
RVH-421 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.1475 0.3378 0.0091 0.0065 0.0088 0.0135 0.0177 0.2227 0.0144 0.0697 0.0135 0.0055 0.0057 0.0185 0.0044 0.0389 0.0128 0.1117 0.0514
RVH-421 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.1855 0.4854 0.0126 0.0105 0.0085 0.0183 0.0240 0.2053 0.0190 0.1093 0.0149 0.0075 0.0062 0.0225 0.0061 0.0464 0.0183 0.0101 0.1504 0.0484
RVH-421 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.2315 0.4167 0.0092 0.0072 0.0090 0.0140 0.0231 0.2160 0.0166 0.0829 0.0238 0.0069 0.0058 0.0167 0.0061 0.0273 0.0252 0.0102 0.0927 0.0526
RVH-421 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.2703 0.5465 0.0125 0.0094 0.0089 0.0196 0.0257 0.1869 0.0232 0.1621 0.0240 0.0086 0.0060 0.0191 0.0098 0.0347 0.0190 0.0094 0.1105 0.0587
RVH-421 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.1642 0.3175 0.0054 0.0052 0.0070 0.0112 0.0133 0.2222 0.0109 0.0944 0.0103 0.0061 0.0057 0.0132 0.0039 0.0417 0.0161 0.0133 0.1534 0.0275
RVH-421 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.1876 0.4065 0.0102 0.0090 0.0078 0.0145 0.0168 0.1792 0.0134 0.1264 0.0139 0.0081 0.0057 0.0127 0.0068 0.0511 0.0131 0.0145 0.1689 0.0380
RVH-421 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.2227 0.4425 0.0150 0.0112 0.0096 0.0135 0.0238 0.1724 0.0159 0.0799 0.0314 0.0069 0.0049 0.0227 0.0066 0.0279 0.0159 0.0089 0.0894 0.0668
RVH-421 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.2433 0.5025 0.0121 0.0088 0.0089 0.0171 0.0249 0.2066 0.0141 0.1044 0.0330 0.0068 0.0048 0.0227 0.0091 0.0330 0.0144 0.0093 0.1136 0.0546