Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.1825 0.5988 0.0086 0.0082 0.0079 0.0225 0.0127 0.1264 0.0096 0.2597 0.0170 0.0064 0.0166 0.0077 0.0138 0.0069 0.0057 0.0273
RVH-421 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.1085 0.4808 0.0129 0.0115 0.0167 0.0175 0.0160 0.0923 0.0177 0.2481 0.0194 0.0099 0.0043 0.0153 0.0044 0.0366 0.0238 0.0129 0.0654 0.0395
RVH-421 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.1142 0.4902 0.0108 0.0097 0.0155 0.0250 0.0184 0.1031 0.0197 0.3049 0.0193 0.0119 0.0050 0.0153 0.0056 0.0398 0.0142 0.0125 0.0646 0.0418
RVH-421 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0920 0.5952 0.0054 0.0045 0.0075 0.0139 0.0085 0.1127 0.0086 0.2193 0.0188 0.0109 0.0044 0.0181 0.0033 0.0142 0.0168 0.0047 0.0385 0.0299
RVH-421 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.1119 0.5882 0.0065 0.0066 0.0073 0.0180 0.0097 0.0861 0.0100 0.2278 0.0131 0.0121 0.0052 0.0187 0.0049 0.0121 0.0087 0.0038 0.0384 0.0373
RVH-421 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.1048 0.5076 0.0160 0.0133 0.0141 0.0256 0.0258 0.1025 0.0280 0.1423 0.0168 0.0105 0.0046 0.0117 0.0036 0.0212 0.0218 0.0085 0.0364 0.0308
RVH-421 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.1143 0.5181 0.0153 0.0137 0.0127 0.0366 0.0293 0.1020 0.0343 0.1520 0.0175 0.0116 0.0055 0.0120 0.0047 0.0230 0.0158 0.0029 0.0355 0.0332
RVH-421 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.1464 0.5780 0.0046 0.0042 0.0076 0.0157 0.0082 0.1252 0.0082 0.1639 0.0176 0.0102 0.0041 0.0127 0.0045 0.0124 0.0127 0.0054 0.0248 0.0651
RVH-421 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.6667 0.0081 0.0080 0.0080 0.0203 0.0122 0.0979 0.0105 0.1678 0.0163 0.0121 0.0055 0.0138 0.0064 0.0137 0.0092 0.0054 0.0251 0.0814
RVH-421 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.1189 0.5291 0.0149 0.0117 0.0142 0.0194 0.0217 0.0870 0.0231 0.2591 0.0211 0.0125 0.0049 0.0167 0.0038 0.0267 0.0276 0.0095 0.0522 0.0392
RVH-421 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.1280 0.5587 0.0120 0.0114 0.0097 0.0270 0.0202 0.0843 0.0245 0.2681 0.0211 0.0104 0.0057 0.0165 0.0056 0.0305 0.0254 0.0041 0.0641 0.0860
RVH-421 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.1100 0.4464 0.0082 0.0078 0.0097 0.0159 0.0125 0.1048 0.0135 0.2404 0.0132 0.0093 0.0042 0.0131 0.0034 0.0160 0.0289 0.0052 0.0445 0.0289
RVH-421 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.1076 0.4630 0.0113 0.0089 0.0086 0.0213 0.0158 0.1094 0.0143 0.2660 0.0123 0.0104 0.0050 0.0157 0.0045 0.0185 0.0115 0.0044 0.0417 0.0963
RVH-421 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.1007 0.3597 0.0136 0.0111 0.0142 0.0251 0.0230 0.1198 0.0202 0.2439 0.0152 0.0094 0.0042 0.0087 0.0034 0.0816 0.0333 0.0039 0.0935 0.0239
RVH-421 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.1139 0.4065 0.0146 0.0122 0.0134 0.0356 0.0259 0.1267 0.0260 0.2151 0.0146 0.0095 0.0047 0.0096 0.0044 0.0583 0.0144 0.0083 0.0585 0.0615
RVH-421 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.1399 0.5236 0.0051 0.0058 0.0076 0.0148 0.0081 0.1539 0.0082 0.2404 0.0156 0.0102 0.0049 0.0127 0.0042 0.0152 0.0184 0.0050 0.0294 0.1044
RVH-421 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.5405 0.0082 0.0070 0.0088 0.0190 0.0123 0.1193 0.0092 0.2755 0.0195 0.0133 0.0061 0.0128 0.0174 0.0105 0.0063 0.0306 0.1799
RVH-421 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0979 0.4566 0.0125 0.0104 0.0111 0.0175 0.0187 0.0941 0.0176 0.2320 0.0151 0.0095 0.0047 0.0155 0.0032 0.0335 0.0144 0.0170 0.0607 0.0337
RVH-421 0.0000
uM
 1
hr
 5
InnoScan/Mapix
RVH-421 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.1412 0.5051 0.0054 0.0044 0.0081 0.0144 0.0095 0.1218 0.0104 0.3690 0.0149 0.0111 0.0049 0.0177 0.0043 0.0190 0.0077 0.0075 0.0498 0.0827
RVH-421 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.1629 0.5587 0.0057 0.0059 0.0071 0.0189 0.0105 0.1198 0.0084 0.3003 0.0160 0.0121 0.0059 0.0180 0.0059 0.0233 0.0081 0.0051 0.0512 0.0831
RVH-421 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.0962 0.4878 0.0143 0.0122 0.0148 0.0257 0.0263 0.1120 0.0277 0.2096 0.0130 0.0135 0.0046 0.0115 0.0032 0.0234 0.0137 0.0116 0.0365 0.0285
RVH-421 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.1076 0.5155 0.0189 0.0139 0.0120 0.0360 0.0283 0.1115 0.0218 0.2320 0.0151 0.0118 0.0055 0.0109 0.0039 0.0258 0.0142 0.0025 0.0404 0.0245
RVH-421 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.1751 0.5587 0.0047 0.0040 0.0068 0.0137 0.0084 0.1441 0.0068 0.4808 0.0191 0.0110 0.0049 0.0129 0.0048 0.0140 0.0071 0.0066 0.0312 0.0596
RVH-421 0.0000
uM
 1
hr
 5
InnoScan/Mapix
 0.1898 0.5917 0.0067 0.0058 0.0070 0.0197 0.0091 0.1397 0.0070 0.3663 0.0199 0.0125 0.0065 0.0133 0.0056 0.0099 0.0042 0.0304 0.0555