Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

 Full screen view
Filter results: >>>>
Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
Vemurafenib RVH-421 3.1600
uM
 24
hr
 7
Odyssey/Microvigene
 0.2825 -0.8999 0.8197 -0.4293 0.1890 -0.0234 0.2336 -0.3237 0.2532 -0.0551 0.4878 -0.5496 0.4049 -0.0475 0.2740 -0.4122 0.2160 -1.7692 0.2732 -0.0225 0.5747 0.0591 0.3226 0.0194 0.2439 -0.0149 0.3195 0.0027 0.1818 -0.0315 0.4167 0.3494 0.2320 0.0595 0.4673 -0.3236 0.4566 0.6445
Vemurafenib RVH-421 3.1600
uM
 24
hr
 8
Odyssey/Microvigene
 0.2762 -0.8999 0.7874 -0.4293 0.2169 -0.0234 0.2247 -0.3237 0.2445 -0.0551 0.4902 -0.5496 0.4049 -0.0475 0.2890 -0.4122 0.2618 -1.7692 0.2695 -0.0225 0.6369 0.0591 0.3030 0.0194 0.2268 -0.0149 0.3497 0.0027 0.2049 -0.0315 0.3984 0.3494 0.2500 0.0595 0.4785 -0.3236 0.4566 0.6445
Vemurafenib RVH-421 3.1600
uM
 48
hr
 1
Odyssey/Microvigene
 0.2310 -1.1053 0.5587 -0.6899 0.1626 -0.1886 0.2128 -0.4694 0.1812 -0.1701 0.3906 -0.7098 0.2667 -0.0446 0.2020 -0.4690 0.2353 -1.9174 0.1869 -0.1471 0.3704 -0.1451 0.2950 0.1958 0.1812 -0.1760 0.2451 -0.0022 0.2193 -0.0174 0.4762 0.5770 0.2137 0.2017 0.1558 -1.5612 0.4274 0.9271
Vemurafenib RVH-421 3.1600
uM
 48
hr
 2
Odyssey/Microvigene
 0.2041 -1.1053 0.5348 -0.6899 0.1458 -0.1886 0.2310 -0.4694 0.1522 -0.1701 0.3802 -0.7098 0.2717 -0.0446 0.1792 -0.4690 0.1770 -1.9174 0.1808 -0.1471 0.4237 -0.1451 0.2688 0.1958 0.1855 -0.1760 0.2387 -0.0022 0.2475 -0.0174 0.3636 0.5770 0.1742 0.2017 0.1623 -1.5612 0.4878 0.9271
Vemurafenib RVH-421 3.1600
uM
 48
hr
 3
Odyssey/Microvigene
 0.2342 -1.1053 0.5618 -0.6899 0.1712 -0.1886 0.2075 -0.4694 0.2128 -0.1701 0.3731 -0.7098 0.3831 -0.0446 0.2433 -0.4690 0.2500 -1.9174 0.2053 -0.1471 0.3937 -0.1451 0.3125 0.1958 0.2020 -0.1760 0.3300 -0.0022 0.1873 -0.0174 0.4854 0.5770 0.2525 0.2017 0.2075 -1.5612 0.5495 0.9271
Vemurafenib RVH-421 3.1600
uM
 48
hr
 4
Odyssey/Microvigene
 0.2155 -1.1053 0.5435 -0.6899 0.1608 -0.1886 0.2049 -0.4694 0.2252 -0.1701 0.3690 -0.7098 0.4115 -0.0446 0.2242 -0.4690 0.1992 -1.9174 0.2041 -0.1471 0.3831 -0.1451 0.3333 0.1958 0.1996 -0.1760 0.3086 -0.0022 0.2105 -0.0174 0.3663 0.5770 0.2188 0.2017 0.2079 -1.5612 0.5435 0.9271
Vemurafenib RVH-421 3.1600
uM
 48
hr
 5
Odyssey/Microvigene
 0.3247 -1.1053 0.9259 -0.6899 0.1883 -0.1886 0.2304 -0.4694 0.2475 -0.1701 0.4310 -0.7098 0.4065 -0.0446 0.3774 -0.4690 0.2660 -1.9174 0.2421 -0.1471 0.5181 -0.1451 0.3509 0.1958 0.2695 -0.1760 0.3472 -0.0022 0.1842 -0.0174 0.4831 0.5770 0.2017 -1.5612 0.5465 0.9271
Vemurafenib RVH-421 3.1600
uM
 48
hr
 6
Odyssey/Microvigene
 0.2451 -1.1053 0.8333 -0.6899 0.1669 -0.1886 0.2123 -0.4694 0.1969 -0.1701 0.4505 -0.7098 0.4049 -0.0446 0.2551 -0.4690 0.2252 -1.9174 0.2488 -0.1471 0.5435 -0.1451 0.3425 0.1958 0.2088 -0.1760 0.3215 -0.0022 0.2066 -0.0174 0.4274 0.5770 0.2381 0.2017 0.2304 -1.5612 0.6289 0.9271
Vemurafenib RVH-421 3.1600
uM
 48
hr
 7
Odyssey/Microvigene
 0.2809 -1.1053 1.0363 -0.6899 0.2075 -0.1886 0.2101 -0.4694 0.3086 -0.1701 0.4695 -0.7098 0.4367 -0.0446 0.2882 -0.4690 0.2222 -1.9174 0.2809 -0.1471 0.6211 -0.1451 0.3745 0.1958 0.2463 -0.1760 0.3846 -0.0022 0.1898 -0.0174 0.5000 0.5770 0.2793 0.2017 0.2227 -1.5612 0.6536 0.9271
Vemurafenib RVH-421 3.1600
uM
 48
hr
 8
Odyssey/Microvigene
 0.2717 -1.1053 1.0010 -0.6899 0.2421 -0.1886 0.2016 -0.4694 0.2564 -0.1701 0.5025 -0.7098 0.4651 -0.0446 0.3460 -0.4690 0.2571 -1.9174 0.2924 -0.1471 0.7299 -0.1451 0.3846 0.1958 0.3049 -0.1760 0.4032 -0.0022 0.1678 -0.0174 0.4739 0.5770 0.2519 0.2017 0.2179 -1.5612 0.6289 0.9271
RVH-421 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.1206 0.4444 0.0115 0.0096 0.0106 0.0152 0.0134 0.1088 0.0162 0.1942 0.0156 0.0089 0.0044 0.0169 0.0038 0.0250 0.0134 0.0112 0.0476 0.0425
RVH-421 0.0000
uM
 1
hr
 1
InnoScan/Mapix
RVH-421 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.1067 0.5525 0.0039 0.0048 0.0080 0.0133 0.0087 0.0904 0.0097 0.5236 0.0127 0.0108 0.0052 0.0192 0.0029 0.0210 0.0081 0.0082 0.0500 0.0351
RVH-421 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.1146 0.6024 0.0076 0.0076 0.0083 0.0179 0.0114 0.0905 0.0085 0.4115 0.0150 0.0113 0.0059 0.0222 0.0044 0.0250 0.0100 0.0059 0.0516 0.0347
RVH-421 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.1014 0.8265 0.0082 0.0076 0.0106 0.0175 0.0148 0.1036 0.0175 0.3677 0.0116 0.0131 0.0055 0.0237 0.0029 0.0282 0.0109 0.0747 0.0306
RVH-421 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.1071 0.9091 0.0116 0.0094 0.0095 0.0246 0.0162 0.0999 0.0169 0.3704 0.0138 0.0128 0.0069 0.0245 0.0036 0.0310 0.0119 0.0061 0.0733 0.0239
RVH-421 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.1558 0.5587 0.0048 0.0034 0.0070 0.0130 0.0085 0.1115 0.0079 0.2695 0.0149 0.0108 0.0049 0.0160 0.0041 0.0156 0.0068 0.0057 0.0336 0.0639
RVH-421 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.1684 0.6289 0.0072 0.0073 0.0070 0.0186 0.0111 0.1167 0.0073 0.2315 0.0165 0.0126 0.0062 0.0167 0.0154 0.0090 0.0053 0.0302
RVH-421 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.1414 0.4032 0.0126 0.0135 0.0117 0.0217 0.0184 0.1138 0.0195 0.2137 0.0255 0.0091 0.0037 0.0165 0.0043 0.0250 0.0155 0.0100 0.0523 0.0535
RVH-421 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.1407 0.4484 0.0147 0.0123 0.0100 0.0311 0.0218 0.1073 0.0209 0.2475 0.0102 0.0043 0.0148 0.0074 0.0252 0.0124 0.0030 0.0495 0.0498
RVH-421 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.1025 0.5376 0.0065 0.0054 0.0105 0.0153 0.0098 0.1009 0.0138 0.3012 0.0128 0.0110 0.0042 0.0177 0.0029 0.0173 0.0082 0.0085 0.0580 0.0285
RVH-421 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.1038 0.5525 0.0087 0.0087 0.0111 0.0210 0.0120 0.0948 0.0114 0.3003 0.0129 0.0106 0.0054 0.0190 0.0047 0.0180 0.0097 0.0071 0.0618 0.0354
RVH-421 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.1098 0.5208 0.0107 0.0104 0.0128 0.0253 0.0200 0.1058 0.0216 0.2976 0.0171 0.0102 0.0045 0.0136 0.0036 0.0377 0.0098 0.0060 0.0554 0.0396
RVH-421 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.1175 0.5051 0.0158 0.0145 0.0128 0.0367 0.0243 0.0989 0.0223 0.3003 0.0172 0.0109 0.0057 0.0149 0.0051 0.0503 0.0114 0.0045 0.0638 0.0394
RVH-421 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.1600 0.5291 0.0054 0.0044 0.0072 0.0157 0.0091 0.1203 0.0073 0.2857 0.0156 0.0110 0.0045 0.0149 0.0046 0.0132 0.0053 0.0048 0.0277 0.0694