Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0883 0.4630 0.0187 0.0147 0.0867 0.0151 0.1484 0.0146 0.0100 0.0038 0.0150 0.0051 0.0156 0.0058 0.0301 0.0321
RVH-421 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0975 0.4785 0.0085 0.0071 0.0084 0.0247 0.0137 0.0996 0.0128 0.1883 0.0157 0.0104 0.0043 0.0149 0.0040 0.0143 0.0081 0.0035 0.0312 0.0252
RVH-421 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.1337 0.5000 0.0068 0.0047 0.0075 0.0157 0.0088 0.1076 0.0110 0.3215 0.0157 0.0105 0.0043 0.0164 0.0042 0.0210 0.0070 0.0072 0.0375 0.0752
RVH-421 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.1497 0.5155 0.0070 0.0063 0.0067 0.0197 0.0100 0.1043 0.0098 0.3534 0.0158 0.0110 0.0055 0.0164 0.0060 0.0173 0.0074 0.0064 0.0395 0.0780
RVH-421 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.0968 0.5556 0.0059 0.0063 0.0081 0.0161 0.0102 0.0973 0.0110 0.1815 0.0133 0.0113 0.0042 0.0121 0.0037 0.0733 0.0062 0.0047 0.0334 0.0494
RVH-421 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.1201 0.5435 0.0066 0.0064 0.0073 0.0209 0.0096 0.1153 0.0104 0.2257 0.0123 0.0113 0.0058 0.0120 0.0041 0.0209 0.0060 0.0043 0.0439 0.0337
RVH-421 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.1629 0.5076 0.0049 0.0040 0.0059 0.0166 0.0079 0.0056 0.3021 0.0175 0.0105 0.0047 0.0119 0.0043 0.0524 0.0055 0.0050 0.0300 0.0203
RVH-421 0.0000
uM
 1
hr
 6
InnoScan/Mapix
 0.1855 0.5495 0.0059 0.0058 0.0073 0.0199 0.0101 0.1066 0.0084 0.3068 0.0156 0.0117 0.0058 0.0122 0.0056 0.0541 0.0069 0.0046 0.0294 0.0207
RVH-421 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.1333 0.5376 0.0096 0.0080 0.0085 0.0143 0.0103 0.1055 0.0109 0.2183 0.0183 0.0103 0.0043 0.0164 0.0044 0.0130 0.0125 0.0051 0.0312 0.0960
RVH-421 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.1515 0.4975 0.0072 0.0055 0.0065 0.0190 0.0098 0.1175 0.0110 0.2770 0.0190 0.0104 0.0049 0.0167 0.0055 0.0132 0.0078 0.0035 0.0346 0.1068
RVH-421 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.1825 0.5128 0.0048 0.0044 0.0067 0.0132 0.0073 0.1403 0.0092 0.4785 0.0210 0.0101 0.0046 0.0175 0.0046 0.0141 0.0141 0.0072 0.0419 0.0402
RVH-421 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.1961 0.5025 0.0055 0.0059 0.0063 0.0172 0.0083 0.1395 0.0078 0.3236 0.0201 0.0107 0.0054 0.0171 0.0060 0.0174 0.0074 0.0059 0.0397 0.0437
RVH-421 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.1555 0.4831 0.0109 0.0074 0.0096 0.0168 0.0140 0.1079 0.0139 0.2008 0.0189 0.0107 0.0043 0.0114 0.0049 0.0118 0.0113 0.0051 0.0243 0.0625
RVH-421 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.1698 0.4608 0.0079 0.0071 0.0080 0.0226 0.0151 0.1060 0.0179 0.2257 0.0180 0.0106 0.0053 0.0112 0.0065 0.0117 0.0084 0.0031 0.0238 0.0934
RVH-421 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.1667 0.5556 0.0046 0.0041 0.0067 0.0143 0.0075 0.1011 0.0067 0.4098 0.0092 0.0106 0.0044 0.0122 0.0040 0.0195 0.0140 0.0060 0.0890 0.0195
RVH-421 0.0000
uM
 1
hr
 7
InnoScan/Mapix
 0.1792 0.5917 0.0067 0.0061 0.0072 0.0193 0.0092 0.0857 0.0116 0.3891 0.0081 0.0060 0.0120 0.0046 0.0084 0.0097 0.0836 0.0267
RVH-421 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.1650 0.6579 0.0061 0.0058 0.0066 0.0125 0.0082 0.1261 0.0073 0.6369 0.0177 0.0113 0.0050 0.0178 0.0044 0.0165 0.0128 0.0069 0.0457 0.0681
RVH-421 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.2004 0.6452 0.0059 0.0046 0.0057 0.0173 0.0079 0.1299 0.0103 0.4566 0.0179 0.0100 0.0055 0.0182 0.0057 0.0214 0.0151 0.0061 0.0536 0.1555
RVH-421 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.1776 0.6757 0.0046 0.0043 0.0073 0.0131 0.0095 0.1027 0.0068 0.6536 0.0119 0.0109 0.0049 0.0182 0.0040 0.0210 0.0229 0.0065 0.0545 0.0170
RVH-421 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.1953 0.6061 0.0052 0.0044 0.0062 0.0181 0.0085 0.1083 0.0071 0.6536 0.0121 0.0108 0.0053 0.0205 0.0046 0.0230 0.0082 0.0053 0.0591 0.0519
RVH-421 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.1898 0.6452 0.0063 0.0045 0.0083 0.0134 0.1092 0.0093 0.3677 0.0120 0.0123 0.0049 0.0140 0.0037 0.0207 0.0089 0.0055 0.0279 0.0254
RVH-421 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.2037 0.6369 0.0053 0.0045 0.0068 0.0162 0.0081 0.1064 0.0112 0.3497 0.0115 0.0109 0.0055 0.0136 0.0048 0.0080 0.0084 0.0337 0.1045
RVH-421 0.0000
uM
 1
hr
 8
InnoScan/Mapix
 0.1838 0.5917 0.0090 0.0250 0.0126 0.0087 0.2217 0.0101 0.0097 0.0049 0.0142 0.0056 0.0173 0.0237 0.0059 0.0399 0.0204
RVH-421 0.0000
uM
 1
hr
 8
InnoScan/Mapix
RVH-421 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.1555 0.7407 0.0070 0.0044 0.0062 0.0072 0.0079 0.2058 0.0077 0.2597 0.0129 0.0103 0.0050 0.0103 0.0039 0.0152 0.0074 0.0067 0.0877 0.1227