Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.2375 0.3718 0.0147 0.0126 0.0099 0.0125 0.0232 0.1789 0.0215 0.2674 0.0313 0.0050 0.0037 0.0137 0.0073 0.0451 0.0200 0.0122 0.1383 0.0592
RVH-421 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.2481 0.3436 0.0124 0.0116 0.0077 0.0158 0.0221 0.1786 0.0197 0.3185 0.0280 0.0049 0.0039 0.0129 0.0082 0.0456 0.0170 0.0047 0.1661 0.0480
RVH-421 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.1294 0.4274 0.0100 0.0098 0.0097 0.0131 0.0205 0.1786 0.0192 0.2033 0.0140 0.0064 0.0046 0.0188 0.0038 0.0225 0.0163 0.0066 0.0940 0.0258
RVH-421 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.1374 0.4405 0.0131 0.0118 0.0083 0.0159 0.0236 0.1647 0.0265 0.2101 0.0136 0.0081 0.0055 0.0163 0.0048 0.0236 0.0139 0.0054 0.0992 0.0288
RVH-421 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.1280 0.4386 0.0113 0.0104 0.0097 0.0136 0.0200 0.1795 0.0178 0.3345 0.0232 0.0064 0.0042 0.0159 0.0038 0.0455 0.0151 0.0110 0.1661 0.0338
RVH-421 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.1325 0.4255 0.0129 0.0111 0.0085 0.0180 0.0202 0.1792 0.0254 0.3731 0.0151 0.0062 0.0052 0.0143 0.0054 0.0473 0.0132 0.0083 0.1859 0.0325
RVH-421 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.1748 0.5051 0.0070 0.0048 0.0056 0.0108 0.0097 0.0077 0.2538 0.0160 0.0074 0.0044 0.0179 0.0043 0.0186 0.0085 0.0066 0.1027 0.0462
RVH-421 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.1883 0.5618 0.0068 0.0054 0.0067 0.0154 0.0109 0.2525 0.0106 0.2494 0.0168 0.0059 0.0194 0.0061 0.0229 0.0083 0.0063 0.0962 0.0554
RVH-421 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.1835 0.4546 0.0104 0.0099 0.0120 0.0222 0.1672 0.0184 0.1779 0.0204 0.0059 0.0044 0.0204 0.0046 0.0226 0.0163 0.0064 0.0890 0.0545
RVH-421 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.1912 0.4951 0.0090 0.0078 0.0071 0.0141 0.0190 0.1770 0.0211 0.1721 0.0196 0.0054 0.0047 0.0189 0.0056 0.0263 0.0172 0.0047 0.0952 0.0837
RVH-421 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.1543 0.4717 0.0083 0.0058 0.0082 0.0101 0.0130 0.2273 0.0129 0.2000 0.0133 0.0063 0.0045 0.0220 0.0039 0.0201 0.0238 0.0053 0.0801 0.0515
RVH-421 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.1739 0.4566 0.0083 0.0071 0.0066 0.0123 0.0158 0.2020 0.0147 0.1795 0.0125 0.0065 0.0051 0.0226 0.0045 0.0279 0.0110 0.0067 0.0791 0.0588
RVH-421 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.1403 0.2801 0.0064 0.0054 0.0075 0.0119 0.0139 0.2024 0.0121 0.2268 0.0129 0.0050 0.0037 0.0103 0.0036 0.0216 0.0117 0.0060 0.1002 0.0331
RVH-421 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.1437 0.2681 0.0062 0.0053 0.0066 0.0136 0.0131 0.2404 0.0153 0.2299 0.0161 0.0050 0.0041 0.0103 0.0040 0.0226 0.0087 0.0050 0.1028 0.0448
RVH-421 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.1776 0.5405 0.0046 0.0044 0.0062 0.0091 0.0088 0.2653 0.0087 0.3096 0.0147 0.0077 0.0049 0.0193 0.0046 0.0346 0.0153 0.0076 0.1144 0.0724
RVH-421 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.1938 0.5882 0.0067 0.0051 0.0061 0.0123 0.0104 0.2066 0.0098 0.3205 0.0155 0.0086 0.0221 0.0066 0.0389 0.0088 0.0067 0.1025 0.0618
RVH-421 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.2331 0.5000 0.0129 0.0122 0.0098 0.0136 0.0250 0.1812 0.0174 0.0734 0.0237 0.0086 0.0060 0.0261 0.0289 0.0178 0.0091 0.0688 0.0712
RVH-421 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.2469 0.5618 0.0136 0.0245 0.0095 0.0177 0.0252 0.1890 0.0158 0.1144 0.0075 0.0057 0.0275 0.0114 0.0296 0.0180 0.0098 0.0990 0.0602
RVH-421 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.1567 0.3953 0.0103 0.0088 0.0111 0.0156 0.0254 0.1692 0.0158 0.0881 0.0172 0.0058 0.0050 0.0185 0.0051 0.0312 0.0155 0.0119 0.1055 0.0549
RVH-421 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.1767 0.4149 0.0161 0.0124 0.0101 0.0218 0.0317 0.1901 0.0175 0.0569 0.0233 0.0052 0.0053 0.0178 0.0079 0.0357 0.0193 0.0094 0.1258 0.0608
RVH-421 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.2257 0.5128 0.0125 0.0098 0.0098 0.0145 0.0265 0.2024 0.0193 0.0862 0.0212 0.0076 0.0062 0.0192 0.0063 0.0260 0.0180 0.0084 0.0727 0.0661
RVH-421 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.2451 0.5128 0.0157 0.0194 0.0085 0.0195 0.0292 0.2463 0.0177 0.1009 0.0288 0.0069 0.0053 0.0187 0.0089 0.0245 0.0206 0.0092 0.1016 0.0694
RVH-421 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.1799 0.2717 0.0085 0.0060 0.0079 0.0117 0.2110 0.0116 0.1073 0.0099 0.0052 0.0046 0.0107 0.0038 0.0334 0.0093 0.0109 0.1112 0.0429
RVH-421 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.1493 0.2370 0.0120 0.0106 0.0082 0.0168 0.2315 0.0136 0.1066 0.0142 0.0048 0.0050 0.0094 0.0056 0.0340 0.0122 0.0101 0.1290 0.0614
RVH-421 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.2088 0.4255 0.0100 0.0081 0.0088 0.0146 0.0181 0.1764 0.0146 0.0613 0.0232 0.0073 0.0046 0.0193 0.0066 0.0291 0.0138 0.0088 0.0672 0.0575