Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

 Full screen view
Filter results: >>>>
Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 48
hr
 8
InnoScan/Mapix
RVH-421 0.0000
uM
 48
hr
 8
InnoScan/Mapix
RVH-421 0.0000
uM
 48
hr
 8
InnoScan/Mapix
RVH-421 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.1965 0.4444 0.0047 0.0043 0.0065 0.0125 0.0096 0.1965 0.0094 0.1028 0.0071 0.0067 0.0050 0.0188 0.0042 0.0307 0.0192 0.0099 0.1179 0.0325
RVH-421 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.2257 0.5814 0.0071 0.0051 0.0072 0.0133 0.0143 0.1770 0.0089 0.1292 0.0076 0.0077 0.0057 0.0211 0.0051 0.0387 0.0125 0.0109 0.1341 0.0479
RVH-421 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.4951 1.2107 0.1613 0.2778 0.3086 0.9346 0.3846 0.3040 0.8696 0.2994 0.4878 0.2941 0.2506 0.2994 0.1767 0.3497 0.1805 0.5848 0.2778
RVH-421 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.4525 1.1976 0.1838 0.2793 0.3367 0.9434 0.4348 0.3378 0.8475 0.2762 0.5319 0.3185 0.2469 0.3115 0.2004 0.3401 0.2146 0.5814 0.2564
RVH-421 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.4587 1.0905 0.1724 0.2817 0.3290 0.8403 0.3731 0.2916 0.8265 0.2320 0.4444 0.2639 0.2049 0.2950 0.1812 0.2941 0.1887 0.5348 0.2481
RVH-421 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.4831 1.1086 0.1575 0.2445 0.3247 0.8547 0.4149 0.3268 0.8547 0.2532 0.5155 0.3012 0.2445 0.3175 0.1912 0.3333 0.2049 0.5747 0.2525
RVH-421 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.4587 1.0661 0.1715 0.2584 0.3677 0.9174 0.3831 0.3030 0.8265 0.2506 0.4608 0.2890 0.2045 0.3012 0.1931 0.3677 0.1969 0.4739 0.2747
RVH-421 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.4405 1.1534 0.1984 0.2625 0.3247 0.9091 0.4505 0.3425 0.8772 0.2667 0.5376 0.3300 0.2237 0.2959 0.1946 0.2193 0.4367 0.2551
RVH-421 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.4348 0.9615 0.1499 0.2469 0.2525 0.7692 0.4202 0.2874 0.8621 0.1996 0.4032 0.2273 0.1828 0.2024 0.3257 0.2141 0.4630 0.2506
RVH-421 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.4505 0.9346 0.1969 0.2268 0.2632 0.7634 0.3906 0.3236 0.8772 0.2188 0.4505 0.2725 0.2070 0.2994 0.1733 0.3484 0.5405 0.2494
RVH-421 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.5051 1.1862 0.1825 0.2525 0.3215 0.9346 0.4115 0.3030 0.8475 0.2577 0.4651 0.2825 0.2370 0.3135 0.1795 0.3597 0.1883 0.5650 0.2786
RVH-421 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.4926 1.0799 0.1969 0.2538 0.3311 0.9615 0.4202 0.3215 0.8403 0.2681 0.5848 0.2833 0.2359 0.2849 0.1645 0.3279 0.2024 0.5618 0.2786
RVH-421 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.4975 1.0684 0.1912 0.2532 0.4630 0.8547 0.4000 0.2985 0.8475 0.2433 0.4762 0.2874 0.2110 0.2849 0.1984 0.3247 0.1873 0.5882 0.2646
RVH-421 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.4926 1.0881 0.2004 0.2525 0.4854 0.9615 0.4167 0.3546 0.8265 0.2625 0.5587 0.3049 0.2506 0.3311 0.1855 0.3356 0.2519 0.6410 0.2778
RVH-421 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.4808 1.1919 0.1815 0.2387 0.3559 0.9901 0.4386 0.3534 0.9524 0.2833 0.5102 0.2994 0.2183 0.3460 0.1770 0.3831 0.2075 0.5181 0.3175
RVH-421 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.4762 1.2626 0.2179 0.2375 0.3663 1.0515 0.4651 0.3984 0.9259 0.2833 0.5882 0.2916 0.2247 0.3584 0.1689 0.4149 0.2375 0.5181 0.3040
RVH-421 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 1.0050 0.2262 0.2994 0.8333 0.3663 0.3185 0.8772 0.2128 0.4587 0.1799 0.2841 0.1678 0.3333 0.2128 0.4902 0.2558
RVH-421 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.4854 1.0438 0.1815 0.2584 0.3257 0.8696 0.4032 0.3984 0.9615 0.2364 0.5000 0.3021 0.2151 0.3021 0.1828 0.3677 0.2890 0.6135 0.2833
RVH-421 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.4951 1.1737 0.1686 0.9174 0.4132 0.3205 0.8621 0.2717 0.5155 0.2950 0.2809 0.2950 0.1650 0.3788 0.2004 0.5917 0.3040
RVH-421 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.4608 1.0526 0.1686 0.2421 0.8929 0.3704 0.3226 0.7634 0.2688 0.5556 0.2717 0.2660 0.2681 0.1855 0.3195 0.1938 0.6173 0.3003
RVH-421 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.4673 0.9174 0.1466 0.2674 0.2778 0.7937 0.3597 0.2865 0.8000 0.2262 0.4525 0.2463 0.2174 0.2865 0.1969 0.2899 0.1876 0.5405 0.3115
RVH-421 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.4695 0.9524 0.1582 0.2299 0.2674 0.7519 0.3636 0.3356 0.8265 0.2469 0.5000 0.2849 0.2359 0.2778 0.1852 0.3125 0.2278 0.5780 0.2959