Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.5025 1.3158 0.2024 0.2488 0.3290 1.0000 0.4184 0.3356 0.8065 0.2874 0.5495 0.3058 0.2347 0.1880 0.3690 0.1835 0.5181 0.3058
RVH-421 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.4878 1.1429 0.2128 0.2475 0.3390 0.9174 0.4032 0.3831 0.8403 0.2488 0.6250 0.3311 0.2421 0.3003 0.1712 0.3731 0.2237 0.5348 0.2849
RVH-421 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.4717 0.9709 0.1539 0.2519 0.2874 0.8130 0.3704 0.3195 0.8850 0.2262 0.4386 0.2857 0.1832 0.2874 0.1942 0.3058 0.1730 0.4902 0.2786
RVH-421 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.4831 0.9709 0.1942 0.2494 0.2959 0.8130 0.4016 0.3774 0.2427 0.4673 0.3068 0.2212 0.3195 0.1905 0.3968 0.2151 0.5348 0.2770
RVH-421 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.5263 0.1949 0.2740 0.3226 0.9524 0.4115 0.3215 1.0235 0.2786 0.5587 0.3322 0.2404 0.3096 0.1927 0.3891 0.1880 0.6135 0.3030
RVH-421 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.5076 1.1390 0.1916 0.2703 0.3185 0.9009 0.4367 0.3322 0.7752 0.3145 0.5988 0.3135 0.2538 0.3345 0.1825 0.3817 0.2625 0.6410 0.3367
RVH-421 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.4926 0.9434 0.1656 0.2604 0.2725 0.7874 0.3953 0.2907 0.8929 0.2532 0.4785 0.2710 0.2101 0.3012 0.2075 0.3195 0.1957 0.5495 0.2899
RVH-421 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.5348 1.0482 0.1835 0.2571 0.3125 0.8333 0.4098 0.3135 0.8130 0.2597 0.5291 0.2890 0.2451 0.2967 0.1815 0.3484 0.2375 0.5747 0.3021
RVH-421 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.5000 1.0764 0.1773 0.2506 0.3745 0.9259 0.4348 0.3597 1.0526 0.2538 0.5319 0.3145 0.2427 0.1912 0.3759 0.2375 0.5780 0.3876
RVH-421 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.5525 1.1249 0.2475 0.3891 0.9174 0.4367 0.3472 0.8696 0.2770 0.5587 0.3690 0.2674 0.3390 0.1898 0.4098 0.2451 0.5650 0.3322
RVH-421 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.4673 0.9091 0.1742 0.2577 0.2899 0.7576 0.3861 0.2801 0.8403 0.2268 0.4237 0.2959 0.2105 0.2924 0.1957 0.3497 0.2096 0.4878 0.2667
RVH-421 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.5128 1.0650 0.1880 0.2538 0.3436 0.8265 0.4132 0.3425 0.9009 0.2381 0.4310 0.3115 0.2128 0.3003 0.2008 0.2976 0.3096 0.5495 0.2994
RVH-421 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.4808 1.1696 0.1996 0.2755 0.3145 1.0070 0.3891 0.3390 0.8475 0.3030 0.5076 0.2985 0.2137 0.3086 0.1894 0.3846 0.2000 0.6452 0.2688
RVH-421 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.4608 1.1614 0.1988 0.3049 0.3817 1.0000 0.4808 0.3125 0.8475 0.2725 0.5102 0.3610 0.2387 0.3106 0.2188 0.3876 0.2457 0.6452 0.2924
RVH-421 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.4785 0.9901 0.1898 0.2916 0.2604 0.8547 0.3906 0.2882 0.7813 0.2500 0.4386 0.2732 0.2041 0.2755 0.1942 0.3300 0.2128 0.6211 0.2545
RVH-421 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.4785 1.0030 0.2053 0.2591 0.2849 0.8696 0.4292 0.3226 0.8065 0.2439 0.5236 0.3257 0.2304 0.3096 0.1969 0.3509 0.2252 0.6849 0.2618
RVH-421 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.4673 1.1416 0.1862 0.2849 0.3106 0.9615 0.4082 0.3106 0.8850 0.2825 0.5291 0.3155 0.2105 0.3226 0.1795 0.3968 0.2410 0.5882 0.2618
RVH-421 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.4695 1.1236 0.2114 0.2695 0.3390 1.0277 0.3497 0.8772 0.2882 0.5682 0.3268 0.2506 0.3257 0.2075 0.4000 0.2591 0.5587 0.2899
RVH-421 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.9346 0.1684 0.2532 0.2667 0.7937 0.3584 0.3068 0.8333 0.2237 0.3831 0.2770 0.1812 0.2740 0.1923 0.3195 0.2075 0.4785 0.2392
RVH-421 0.0000
uM
 1
hr
 5
Odyssey/Microvigene
 0.4651 0.9259 0.1592 0.2421 0.2725 0.7874 0.4082 0.3145 0.8696 0.2320 0.4630 0.2994 0.2110 0.2786 0.1862 0.3571 0.2217 0.5618 0.2500
RVH-421 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.4854 1.1507 0.1812 0.2747 0.3115 0.9346 0.3831 0.3571 0.8065 0.2710 0.4673 0.2747 0.2304 0.2710 0.1792 0.3448 0.2353 0.6135 0.2667
RVH-421 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.4831 1.0341 0.1832 0.2681 0.3345 0.9709 0.3906 0.3436 0.8197 0.2639 0.5376 0.2532 0.2105 0.2604 0.1727 0.2933 0.2545 0.5814 0.2653
RVH-421 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.4566 0.9524 0.1672 0.2558 0.2632 0.8333 0.3861 0.2907 0.8403 0.2331 0.4348 0.2732 0.1908 0.2874 0.1828 0.3236 0.1984 0.5848 0.2674
RVH-421 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.4630 0.9804 0.1880 0.2427 0.2959 0.8772 0.3953 0.3185 0.8197 0.2439 0.5291 0.2695 0.2257 0.3049 0.1880 0.3333 0.2237 0.6173 0.2688
RVH-421 0.0000
uM
 1
hr
 6
Odyssey/Microvigene
 0.4739 1.0091 0.1770 0.2558 0.3030 0.8929 0.3861 0.2985 0.8772 0.2688 0.4348 0.2950 0.2028 0.2747 0.1802 0.3521 0.1894 0.5882 0.3049