Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.1437 0.5882 0.0100 0.0097 0.0097 0.0115 0.0182 0.1495 0.0172 0.3268 0.0189 0.0078 0.0042 0.0059 0.0051 0.0218 0.0342 0.0096 0.0983 0.0359
RVH-421 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.1946 0.6369 0.0137 0.0121 0.0088 0.0147 0.0218 0.1346 0.0215 0.3185 0.0184 0.0085 0.0049 0.0083 0.0065 0.0257 0.0177 0.0053 0.1002 0.0707
RVH-421 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.1086 0.3497 0.0059 0.0052 0.0077 0.0117 0.0108 0.1667 0.0122 0.2353 0.0144 0.0066 0.0036 0.0045 0.0036 0.0177 0.0208 0.0056 0.0643 0.0238
RVH-421 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.1163 0.3584 0.0076 0.0064 0.0071 0.0137 0.0132 0.1835 0.0153 0.2421 0.0146 0.0064 0.0040 0.0049 0.0047 0.0138 0.0102 0.0048 0.0661 0.0588
RVH-421 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.1479 0.6024 0.0053 0.0054 0.0071 0.0103 0.0087 0.2203 0.0096 0.2681 0.0167 0.0083 0.0050 0.0083 0.0043 0.0194 0.0164 0.0054 0.0750 0.1473
RVH-421 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.1789 0.6536 0.0081 0.0083 0.0076 0.0129 0.0136 0.1758 0.0106 0.2755 0.0223 0.0055 0.0089 0.0070 0.0183 0.0113 0.0059 0.0580 0.1148
RVH-421 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.2041 0.6711 0.0107 0.0126 0.0107 0.0122 0.0232 0.1650 0.0233 0.4065 0.0270 0.0099 0.0047 0.0087 0.0057 0.0520 0.0186 0.1572 0.0650
RVH-421 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.2315 0.7143 0.0163 0.0126 0.0076 0.0146 0.0222 0.1672 0.0210 0.5319 0.0103 0.0053 0.0084 0.0493 0.0145 0.0176 0.2114 0.0440
RVH-421 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.1223 0.5291 0.0072 0.0063 0.0079 0.0098 0.0143 0.1307 0.0145 0.3185 0.0121 0.0092 0.0050 0.0085 0.0037 0.0173 0.0094 0.0080 0.0784 0.0293
RVH-421 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.1236 0.6452 0.0099 0.0078 0.0068 0.0117 0.0150 0.1208 0.0133 0.2445 0.0129 0.0101 0.0060 0.0095 0.0043 0.0174 0.0106 0.0044 0.0842 0.0268
RVH-421 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.1143 0.3623 0.0094 0.0112 0.0103 0.0125 0.0220 0.1639 0.0223 0.2079 0.0131 0.0078 0.0038 0.0060 0.0038 0.0246 0.0142 0.0095 0.0792 0.0327
RVH-421 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.1264 0.4032 0.0127 0.0112 0.0082 0.0158 0.0216 0.1712 0.0195 0.2416 0.0141 0.0076 0.0045 0.0054 0.0043 0.0270 0.0138 0.0037 0.0875 0.0233
RVH-421 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.1669 0.5747 0.0054 0.0042 0.0058 0.0083 0.0097 0.1515 0.0081 0.2359 0.0145 0.0089 0.0048 0.0087 0.0042 0.0126 0.0066 0.0051 0.0465 0.0920
RVH-421 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.1715 0.6061 0.0069 0.0060 0.0057 0.0107 0.0099 0.1479 0.0077 0.1795 0.0144 0.0099 0.0061 0.0088 0.0059 0.0156 0.0082 0.0039 0.0440 0.1130
RVH-421 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.2058 0.4386 0.0143 0.0137 0.0100 0.0129 0.0240 0.1458 0.0199 0.2778 0.0281 0.0070 0.0042 0.0066 0.0067 0.0397 0.1215 0.0550
RVH-421 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.2155 0.4608 0.0110 0.0106 0.0075 0.0161 0.0217 0.1550 0.0185 0.0300 0.0069 0.0041 0.0062 0.0081 0.0336 0.0129 0.0051 0.1256 0.0548
RVH-421 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.1143 0.6494 0.0082 0.0061 0.0085 0.0099 0.0124 0.1387 0.0176 0.2123 0.0131 0.0096 0.0051 0.0101 0.0035 0.0151 0.0093 0.0057 0.0587 0.0295
RVH-421 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.2028 0.6803 0.0088 0.0078 0.0066 0.0120 0.0134 0.1418 0.0135 0.2342 0.0124 0.0100 0.0056 0.0100 0.0049 0.0137 0.0092 0.0043 0.0650 0.0348
RVH-421 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.1172 0.4329 0.0173 0.0085 0.0095 0.0130 0.0190 0.1418 0.0185 0.1678 0.0153 0.0071 0.0036 0.0058 0.0038 0.0142 0.0096 0.0061 0.0810 0.0351
RVH-421 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.1188 0.4673 0.0117 0.0094 0.0076 0.0151 0.0190 0.1488 0.0171 0.1953 0.0149 0.0080 0.0044 0.0059 0.0054 0.0126 0.0089 0.0038 0.0821 0.0377
RVH-421 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.1425 0.5435 0.0048 0.0054 0.0063 0.0095 0.0084 0.1681 0.0077 0.2062 0.0143 0.0081 0.0041 0.0078 0.0042 0.0136 0.0053 0.0046 0.0518 0.0557
RVH-421 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.1686 0.5556 0.0064 0.0061 0.0057 0.0135 0.0106 0.1647 0.0082 0.2179 0.0154 0.0093 0.0056 0.0076 0.0063 0.0140 0.0070 0.0042 0.0479 0.0713
RVH-421 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.2625 0.6024 0.0163 0.0182 0.0117 0.0152 0.1647 0.0322 0.3145 0.0412 0.0074 0.0040 0.0114 0.0080 0.0413 0.0280 0.1458 0.0683
RVH-421 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.2747 0.5848 0.0123 0.0121 0.0086 0.0177 0.0308 0.1570 0.0240 0.3774 0.0387 0.0073 0.0044 0.0071 0.0489 0.0192 0.0019 0.1405 0.0632
RVH-421 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.1297 0.6173 0.0102 0.0084 0.0085 0.0109 0.0155 0.1205 0.0165 0.2247 0.0129 0.0087 0.0047 0.0083 0.0033 0.0133 0.0177 0.0053 0.0522 0.0235