Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.1203 0.5988 0.0124 0.0096 0.0069 0.0128 0.0183 0.1276 0.0203 0.2028 0.0125 0.0086 0.0055 0.0075 0.0042 0.0120 0.0110 0.0037 0.0565 0.0337
RVH-421 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.1220 0.5025 0.0123 0.0130 0.0106 0.0131 0.0235 0.1272 0.0233 0.2028 0.0170 0.0077 0.0036 0.0062 0.0039 0.0219 0.0183 0.0083 0.0779 0.0305
RVH-421 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.1269 0.5025 0.0146 0.0119 0.0079 0.0146 0.0230 0.1377 0.0316 0.2331 0.0153 0.0075 0.0045 0.0061 0.0056 0.0220 0.0134 0.0037 0.0877 0.0328
RVH-421 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.1486 0.7634 0.0048 0.0045 0.0058 0.0079 0.0083 0.1828 0.0081 0.3300 0.0157 0.0092 0.0050 0.0100 0.0049 0.0368 0.0124 0.0057 0.0769 0.0490
RVH-421 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.1742 0.7937 0.0072 0.0060 0.0057 0.0118 0.0100 0.1515 0.0111 0.3175 0.0159 0.0061 0.0098 0.0060 0.0191 0.0073 0.0060 0.0711 0.0569
RVH-421 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.2096 0.4902 0.0139 0.0144 0.0135 0.1306 0.0289 0.1707 0.0250 0.0075 0.0043 0.0066 0.0053 0.0159 0.0228 0.0065 0.0624 0.0428
RVH-421 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.2075 0.5181 0.0131 0.0116 0.0072 0.0159 0.0268 0.1341 0.0298 0.1672 0.0238 0.0068 0.0044 0.0068 0.0184 0.0284 0.0035 0.0669 0.0749
RVH-421 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.1344 0.6024 0.0066 0.0062 0.0079 0.0093 0.0127 0.1339 0.0125 0.3096 0.0139 0.0080 0.0045 0.0070 0.0034 0.0174 0.0264 0.0072 0.0786 0.0306
RVH-421 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.1335 0.5988 0.0087 0.0072 0.0060 0.0113 0.0152 0.1277 0.0139 0.2924 0.0123 0.0090 0.0047 0.0085 0.0040 0.0181 0.0113 0.0057 0.0854 0.0791
RVH-421 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.1085 0.4785 0.0063 0.0047 0.0065 0.0082 0.0094 0.1539 0.0088 0.1395 0.0123 0.0075 0.0040 0.0066 0.0028 0.0121 0.0083 0.0042 0.0403 0.0253
RVH-421 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.1218 0.4717 0.0052 0.0046 0.0052 0.0097 0.0095 0.1739 0.0106 0.1451 0.0114 0.0077 0.0044 0.0066 0.0040 0.0105 0.0069 0.0033 0.0527 0.0688
RVH-421 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.1458 0.6211 0.0045 0.0043 0.0057 0.0085 0.0074 0.2045 0.0072 0.2232 0.0128 0.0084 0.0043 0.0085 0.0037 0.0138 0.0147 0.0049 0.0694 0.0827
RVH-421 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.1678 0.6250 0.0063 0.0047 0.0056 0.0115 0.0092 0.1730 0.0081 0.2336 0.0127 0.0101 0.0085 0.0050 0.0143 0.0086 0.0047 0.0681 0.1300
RVH-421 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.1119 0.3690 0.0080 0.0054 0.0086 0.0121 0.0122 0.1135 0.0122 0.6024 0.0129 0.0063 0.0041 0.0228 0.0033 0.0826 0.0118 0.0274 0.2146 0.0410
RVH-421 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.1232 0.4184 0.0076 0.0059 0.0071 0.0160 0.0121 0.1130 0.0093 0.6897 0.0174 0.0077 0.0045 0.0222 0.0067 0.0924 0.0100 0.0168 0.2639 0.0322
RVH-421 0.0000
uM
 10
hr
 1
InnoScan/Mapix
RVH-421 0.0000
uM
 10
hr
 1
InnoScan/Mapix
RVH-421 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.1131 0.2857 0.0120 0.0109 0.0124 0.0176 0.0229 0.1445 0.0262 0.2304 0.0151 0.0060 0.0040 0.0139 0.0032 0.0297 0.0167 0.0105 0.0808 0.0330
RVH-421 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.1267 0.3509 0.0149 0.0144 0.0098 0.0245 0.0294 0.1502 0.0258 0.2519 0.0163 0.0064 0.0047 0.0147 0.0040 0.0332 0.0194 0.0040 0.1179 0.0253
RVH-421 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.1650 0.5952 0.0055 0.0035 0.0061 0.0098 0.0087 0.1590 0.0076 0.3597 0.0082 0.0054 0.0323 0.0050 0.0247 0.0069 0.0058 0.0638 0.0630
RVH-421 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.1980 0.6623 0.0074 0.0072 0.0059 0.0143 0.0117 0.1715 0.0084 0.2639 0.0159 0.0101 0.0063 0.0326 0.0073 0.0259 0.0096 0.0059 0.0527 0.0873
RVH-421 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.1337 0.6897 0.0102 0.0087 0.0091 0.0171 0.0154 0.1341 0.0151 0.4065 0.0159 0.0097 0.0035 0.0263 0.0035 0.0711 0.0115 0.1623 0.0353
RVH-421 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.1218 0.6536 0.0098 0.0083 0.0070 0.0236 0.0153 0.1423 0.0131 0.4695 0.0184 0.0104 0.0037 0.0254 0.0046 0.0714 0.0099 0.0082 0.1825 0.0436
RVH-421 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.1074 0.4546 0.0046 0.0040 0.0061 0.0124 0.0091 0.1410 0.0093 0.2198 0.0132 0.0070 0.0042 0.0282 0.0029 0.0280 0.0073 0.0071 0.0656 0.0481
RVH-421 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.1323 0.4717 0.0062 0.0067 0.0062 0.0168 0.0110 0.1339 0.0093 0.2299 0.0117 0.0075 0.0055 0.0316 0.0044 0.0265 0.0087 0.0048 0.0674 0.0477