Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.1008 0.3077 0.0078 0.0078 0.0157 0.0137 0.1250 0.0162 0.1838 0.0138 0.0061 0.0036 0.0128 0.0031 0.0251 0.0085 0.0071 0.0769 0.0290
RVH-421 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.1075 0.3436 0.0089 0.0104 0.0073 0.0211 0.0147 0.1323 0.0150 0.1957 0.0162 0.0058 0.0045 0.0128 0.0042 0.0177 0.0095 0.0050 0.0805 0.0304
RVH-421 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.1818 0.5181 0.0047 0.0042 0.0057 0.0121 0.0092 0.0072 0.2242 0.0172 0.0076 0.0042 0.0228 0.0047 0.0287 0.0060 0.0045 0.0565 0.0518
RVH-421 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.2000 0.5587 0.0082 0.0074 0.0060 0.0186 0.0118 0.1805 0.0093 0.2165 0.0193 0.0095 0.0054 0.0247 0.0246 0.0093 0.0059 0.0493
RVH-421 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.1276 0.3322 0.0119 0.0101 0.0098 0.0169 0.0192 0.1370 0.0175 0.4425 0.0172 0.0053 0.0032 0.0160 0.0035 0.0755 0.0193 0.1848 0.0379
RVH-421 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.1328 0.3135 0.0138 0.0104 0.0079 0.0250 0.0182 0.1306 0.0186 0.5376 0.0172 0.0056 0.0038 0.0155 0.0054 0.0815 0.0147 0.0073 0.2058 0.0345
RVH-421 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.1018 0.5780 0.0066 0.0045 0.0070 0.0136 0.0096 0.1416 0.0117 0.2571 0.0123 0.0080 0.0042 0.0305 0.0033 0.0314 0.0129 0.0079 0.0922 0.0288
RVH-421 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.1314 0.5848 0.0079 0.0078 0.0066 0.0182 0.0118 0.1233 0.0132 0.2564 0.0125 0.0109 0.0055 0.0318 0.0043 0.0309 0.0094 0.0060 0.0978 0.0418
RVH-421 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.1015 0.3322 0.0103 0.0107 0.0103 0.0163 0.0190 0.1170 0.0216 0.2817 0.0145 0.0057 0.0036 0.0122 0.0031 0.0364 0.0156 0.0124 0.0984 0.0256
RVH-421 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.1079 0.3425 0.0153 0.0131 0.0087 0.0213 0.0237 0.1255 0.0270 0.3021 0.0144 0.0065 0.0045 0.0120 0.0046 0.0398 0.0129 0.0064 0.1119 0.0269
RVH-421 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.1560 0.5435 0.0047 0.0055 0.0062 0.0112 0.0077 0.1957 0.0070 0.2353 0.0141 0.0075 0.0042 0.0228 0.0044 0.0258 0.0111 0.0067 0.0615 0.0657
RVH-421 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.1812 0.6173 0.0073 0.0067 0.0065 0.0148 0.0108 0.1745 0.0086 0.2294 0.0155 0.0128 0.0055 0.0245 0.0067 0.0213 0.0094 0.0075 0.0636
RVH-421 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.1163 0.6536 0.0113 0.0109 0.0110 0.0161 0.0178 0.1199 0.0174 0.3257 0.0153 0.0087 0.0044 0.0213 0.0034 0.0564 0.0184 0.0148 0.1211 0.0310
RVH-421 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.1232 0.5128 0.0089 0.0087 0.0076 0.0218 0.0175 0.1164 0.0221 0.3650 0.0151 0.0080 0.0052 0.0173 0.0046 0.0750 0.0188 0.0062 0.1522 0.0667
RVH-421 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.1146 0.4032 0.0077 0.0079 0.0083 0.0149 0.0126 0.1721 0.0133 0.2257 0.0125 0.0063 0.0039 0.0205 0.0034 0.0305 0.0244 0.0081 0.0752 0.0260
RVH-421 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.1407 0.4444 0.0115 0.0103 0.0079 0.0199 0.0158 0.1590 0.0158 0.2212 0.0125 0.0070 0.0048 0.0253 0.0048 0.0333 0.0123 0.0041 0.0719 0.0436
RVH-421 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.1631 0.2336 0.0108 0.0096 0.0109 0.0185 0.0249 0.1565 0.0193 0.3448 0.0175 0.0050 0.0036 0.0084 0.0043 0.0352 0.0328 0.0114 0.1236 0.0235
RVH-421 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.1880 0.2370 0.0143 0.0106 0.0103 0.0258 0.0255 0.1595 0.0252 0.4219 0.0172 0.0054 0.0040 0.0092 0.0060 0.0336 0.0159 0.0070 0.1433 0.0592
RVH-421 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.1992 0.5263 0.0038 0.0044 0.0058 0.0107 0.0072 0.2252 0.0069 0.2525 0.0142 0.0081 0.0046 0.0198 0.0054 0.0171 0.0150 0.0050 0.0698 0.1570
RVH-421 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.2294 0.5650 0.0065 0.0064 0.0065 0.0136 0.0106 0.1894 0.0086 0.2439 0.0171 0.0098 0.0056 0.0221 0.0081 0.0206 0.0097 0.0050 0.0609 0.1567
RVH-421 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.1097 0.5236 0.0089 0.0058 0.0072 0.0134 0.0120 0.1214 0.0112 0.3311 0.0112 0.0084 0.0048 0.0277 0.0031 0.0645 0.0102 0.0202 0.1217 0.0367
RVH-421 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.1318 0.5848 0.0058 0.0051 0.0064 0.0157 0.0116 0.1391 0.0086 0.4630 0.0173 0.0097 0.0057 0.0254 0.0040 0.0765 0.0078 0.0181 0.1490 0.0222
RVH-421 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.1399 0.5263 0.0078 0.0058 0.0075 0.0144 0.0133 0.1515 0.0129 0.3378 0.0117 0.0082 0.0050 0.0343 0.0032 0.0271 0.0082 0.0069 0.0853 0.0290
RVH-421 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.1366 0.6098 0.0090 0.0070 0.0065 0.0184 0.0143 0.1675 0.0112 0.3040 0.0184 0.0092 0.0059 0.0349 0.0044 0.0300 0.0090 0.0044 0.0869 0.0355
RVH-421 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.1227 0.2793 0.0134 0.0114 0.0173 0.0234 0.1600 0.0244 0.2849 0.0122 0.0067 0.0041 0.0127 0.0034 0.0423 0.0139 0.0167 0.1008 0.0308