Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Gefitinib | MCF 10A | 1.0 | uM | 6 | hr | 188.90 | 143.58 | p-Akt | 26.72 | |
| Gefitinib | MCF 10A | 1.0 | uM | 24 | hr | 200.98 | 35.34 | p-Akt | 25.46 | |
| Torkinib | MCF 10A | 562.0 | nM | 6 | hr | 573.43 | 154.46 | p-Akt | 20.79 | |
| Torkinib | MCF 10A | 562.0 | nM | 24 | hr | 427.65 | 97.49 | p-Akt | 13.14 | |
| GSK1059615 | MCF 10A | 1.0 | uM | 6 | hr | 254.98 | 130.47 | p-Akt | 16.63 | |
| GSK1059615 | MCF 10A | 1.0 | uM | 24 | hr | 259.20 | 88.56 | p-Akt | 14.91 | |
| MK2206 | MCF 10A | 1.0 | uM | 6 | hr | 378.15 | 147.34 | p-Akt | 18.05 | |
| MK2206 | MCF 10A | 1.0 | uM | 24 | hr | 334.73 | 161.59 | p-Akt | 15.73 | |
| Triciribine | MCF 10A | 1.0 | uM | 6 | hr | 380.32 | 148.44 | p-Akt | 18.12 | |
| Triciribine | MCF 10A | 1.0 | uM | 24 | hr | 292.88 | 142.42 | p-Akt | 18.46 | |
| Dactolisib | MCF 10A | 1.0 | uM | 6 | hr | 336.82 | 110.71 | p-Akt | 15.93 | |
| Dactolisib | MCF 10A | 1.0 | uM | 24 | hr | 366.10 | 45.69 | p-Akt | 12.12 | |
| Gefitinib | T47D | 1.0 | uM | 6 | hr | 158.07 | 93.35 | p-Akt | 98.53 | |
| Gefitinib | T47D | 1.0 | uM | 24 | hr | 148.87 | 85.46 | p-Akt | 68.69 | |
| Torkinib | T47D | 562.0 | nM | 6 | hr | 152.84 | 70.14 | p-Akt | 16.03 | |
| Torkinib | T47D | 562.0 | nM | 24 | hr | 127.60 | 40.01 | p-Akt | 14.71 | |
| GSK1059615 | T47D | 1.0 | uM | 6 | hr | 162.77 | 63.84 | p-Akt | 23.45 | |
| GSK1059615 | T47D | 1.0 | uM | 24 | hr | 162.76 | 47.25 | p-Akt | 19.66 | |
| MK2206 | T47D | 1.0 | uM | 6 | hr | 99.03 | 36.33 | p-Akt | 5.91 | |
| MK2206 | T47D | 1.0 | uM | 24 | hr | 134.17 | 48.49 | p-Akt | 14.42 | |
| Triciribine | T47D | 1.0 | uM | 6 | hr | 101.24 | 26.12 | p-Akt | 9.22 | |
| Triciribine | T47D | 1.0 | uM | 24 | hr | 178.44 | 67.80 | p-Akt | 36.23 | |
| Dactolisib | T47D | 1.0 | uM | 6 | hr | 93.74 | 42.44 | p-Akt | 6.74 | |
| Dactolisib | T47D | 1.0 | uM | 24 | hr | 123.49 | 35.99 | p-Akt | 13.58 | |
| Gefitinib | AU565 | 3.16 | uM | 6 | hr | 262.04 | 61.46 | p-Akt | 19.52 | |