Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib IOX2 1.0000 2.6667
uM
 48
hr
 1 2950 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.83 NGFR rabbit IgG(H+L) / donkey NGFR 183.99
A375-A2-rep1
D8
A-375 Vemurafenib Mocetinostat 1.0000 2.6667
uM
 48
hr
 1 304 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 299.57 NGFR rabbit IgG(H+L) / donkey NGFR 87.98
A375-A2-rep1
E4
A-375 Vemurafenib AGK2 1.0000 2.6667
uM
 48
hr
 1 678 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 394.51 NGFR rabbit IgG(H+L) / donkey NGFR 132.96
A375-A2-rep1
D5
A-375 Vemurafenib (-)-JQ1 1.0000 2.6667
uM
 48
hr
 1 3035 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.74 NGFR rabbit IgG(H+L) / donkey NGFR 239.66
A375-A2-rep1
D7
A-375 Vemurafenib Azacitidine 1.0000 2.6667
uM
 48
hr
 1 1210 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 261.75 NGFR rabbit IgG(H+L) / donkey NGFR 53.65
A375-A2-rep1
E2
A-375 Vemurafenib C646 1.0000 2.6667
uM
 48
hr
 1 2172 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.52 NGFR rabbit IgG(H+L) / donkey NGFR 184.53
A375-A2-rep1
E3
A-375 Vemurafenib JW55 1.0000 2.6667
uM
 48
hr
 1 2571 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.73 NGFR rabbit IgG(H+L) / donkey NGFR 138.78
A375-A2-rep1
D3
A-375 Vemurafenib Entinostat 1.0000 2.6667
uM
 48
hr
 1 2292 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 451.09 NGFR rabbit IgG(H+L) / donkey NGFR 264.91
A375-A2-rep1
D4
A-375 Vemurafenib GSK-J4 1.0000 2.6667
uM
 48
hr
 1 1533 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.60 NGFR rabbit IgG(H+L) / donkey NGFR 96.94
A375-A2-rep1
D6
A-375 Vemurafenib Olaparib 1.0000 2.6667
uM
 48
hr
 1 1874 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.99 NGFR rabbit IgG(H+L) / donkey NGFR 198.38
A375-A2-rep1
B2
A-375 Vemurafenib Iniparib 1.0000 2.6667
uM
 48
hr
 1 2408 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 180.32 NGFR rabbit IgG(H+L) / donkey NGFR 193.86
A375-A2-rep1
B3
A-375 Vemurafenib CTB 1.0000 2.6667
uM
 48
hr
 1 2224 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.14 NGFR rabbit IgG(H+L) / donkey NGFR 160.82
A375-A2-rep1
B4
A-375 Vemurafenib Rocilinostat 1.0000 2.6667
uM
 48
hr
 1 2722 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 279.18 NGFR rabbit IgG(H+L) / donkey NGFR 95.39
A375-A2-rep1
B5
A-375 Vemurafenib GSK-J1 1.0000 2.6667
uM
 48
hr
 1 2495 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.65 NGFR rabbit IgG(H+L) / donkey NGFR 225.50
A375-A2-rep1
B6
A-375 Vemurafenib PFI-1 1.0000 2.6667
uM
 48
hr
 1 2255 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.05 NGFR rabbit IgG(H+L) / donkey NGFR 162.18
A375-A2-rep1
B7
A-375 Vemurafenib UNC669 1.0000 2.6667
uM
 48
hr
 1 2298 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.06 NGFR rabbit IgG(H+L) / donkey NGFR 186.36
A375-A2-rep1
B8
A-375 Vemurafenib Veliparib 1.0000 2.6667
uM
 48
hr
 1 2081 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 171.06 NGFR rabbit IgG(H+L) / donkey NGFR 200.69
A375-A2-rep1
C2
A-375 Vemurafenib Rucaparib 1.0000 2.6667
uM
 48
hr
 1 2038 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.07 NGFR rabbit IgG(H+L) / donkey NGFR 172.41
A375-A2-rep1
C3
A-375 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 1 279 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 395.49 NGFR rabbit IgG(H+L) / donkey NGFR 21.95
A375-A2-rep1
C4
A-375 Vemurafenib Selisistat 1.0000 2.6667
uM
 48
hr
 1 2960 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 191.58 NGFR rabbit IgG(H+L) / donkey NGFR 184.54
A375-A2-rep1
C5
A-375 Vemurafenib GSK-J2 1.0000 2.6667
uM
 48
hr
 1 2997 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 181.28 NGFR rabbit IgG(H+L) / donkey NGFR 199.06
A375-A2-rep1
C6
A-375 Vemurafenib (+)-JQ1 1.0000 2.6667
uM
 48
hr
 1 1957 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.74 NGFR rabbit IgG(H+L) / donkey NGFR 79.92
A375-A2-rep1
C7
A-375 Vemurafenib UNC1215 1.0000 2.6667
uM
 48
hr
 1 2539 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.45 NGFR rabbit IgG(H+L) / donkey NGFR 217.23
A375-A2-rep1
C8
A-375 Vemurafenib Vorinostat 1.0000 2.6667
uM
 48
hr
 1 1890 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 229.09 NGFR rabbit IgG(H+L) / donkey NGFR 60.09
A375-A2-rep1
D2
A-375 Vemurafenib Resveratrol 1.0000 2.6667
uM
 48
hr
 1 2505 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 181.88 NGFR rabbit IgG(H+L) / donkey NGFR 186.11
A375-A2-rep1
E5
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