Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Pracinostat 0.3160 2.6667
uM
 48
hr
 2 127 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 200.69 NGFR rabbit IgG(H+L) / donkey NGFR 37.13
Colo858-A2-rep2
F4
A-375 Vemurafenib Pracinostat 1.0000 2.6667
uM
 48
hr
 1 137 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 388.80 NGFR rabbit IgG(H+L) / donkey NGFR 39.73
A375-A2-rep1
F4
COLO 858 Vemurafenib Belinostat 0.3160 2.6667
uM
 48
hr
 2 165 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 207.48 NGFR rabbit IgG(H+L) / donkey NGFR 20.78
Colo858-A2-rep2
C4
COLO 858 Vemurafenib Pracinostat 0.3160 2.6667
uM
 48
hr
 1 169 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 222.43 NGFR rabbit IgG(H+L) / donkey NGFR 28.52
Colo858-A2-rep1
F4
WM-115 Vemurafenib AGK2 1.0000 2.6667
uM
 48
hr
 1 193 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.10 NGFR rabbit IgG(H+L) / donkey NGFR 876.79
WM115-A2-rep1
D5
COLO 858 Vemurafenib Belinostat 0.3160 2.6667
uM
 48
hr
 1 209 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 309.25 NGFR rabbit IgG(H+L) / donkey NGFR 21.49
Colo858-A2-rep1
C4
A-375 Vemurafenib Pracinostat 1.0000 2.6667
uM
 48
hr
 2 215 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 234.28 NGFR rabbit IgG(H+L) / donkey NGFR 35.01
A375-A2-rep2
F4
A-375 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 1 279 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 395.49 NGFR rabbit IgG(H+L) / donkey NGFR 21.95
A375-A2-rep1
C4
A-375 Vemurafenib Mocetinostat 1.0000 2.6667
uM
 48
hr
 1 304 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 299.57 NGFR rabbit IgG(H+L) / donkey NGFR 87.98
A375-A2-rep1
E4
WM-115 Vemurafenib AGK2 1.0000 2.6667
uM
 48
hr
 2 313 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.37 NGFR rabbit IgG(H+L) / donkey NGFR 888.19
WM115-A2-rep2
D5
A-375 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 2 319 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 360.58 NGFR rabbit IgG(H+L) / donkey NGFR 26.94
A375-A2-rep2
C4
COLO 858 Vemurafenib Pracinostat 0.3160 0.5333
uM
 48
hr
 2 326 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 166.82 NGFR rabbit IgG(H+L) / donkey NGFR 26.66
Colo858-B1-rep2
F4
COLO 858 Vemurafenib Vorinostat 0.3160 2.6667
uM
 48
hr
 1 329 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 200.63 NGFR rabbit IgG(H+L) / donkey NGFR 17.50
Colo858-A2-rep1
D2
COLO 858 Vemurafenib Belinostat 0.3160 0.5333
uM
 48
hr
 2 367 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 240.33 NGFR rabbit IgG(H+L) / donkey NGFR 12.62
Colo858-B1-rep2
C4
COLO 858 Vemurafenib Belinostat 0.3160 0.5333
uM
 48
hr
 1 394 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 228.28 NGFR rabbit IgG(H+L) / donkey NGFR 28.69
Colo858-B1-rep1
C4
COLO 858 Vemurafenib Pracinostat 0.3160 0.5333
uM
 48
hr
 1 456 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.16 NGFR rabbit IgG(H+L) / donkey NGFR 34.51
Colo858-B1-rep1
F4
COLO 858 Vemurafenib Vorinostat 0.3160 2.6667
uM
 48
hr
 2 478 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 242.50 NGFR rabbit IgG(H+L) / donkey NGFR 28.47
Colo858-A2-rep2
D2
WM-115 Vemurafenib (+)-JQ1 1.0000 2.6667
uM
 48
hr
 1 521 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.85 NGFR rabbit IgG(H+L) / donkey NGFR 452.89
WM115-A2-rep1
C7
COLO 858 Vemurafenib GSK-J4 0.3160 2.6667
uM
 48
hr
 1 528 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 232.59 NGFR rabbit IgG(H+L) / donkey NGFR 14.66
Colo858-A2-rep1
D6
A-375 Vemurafenib Mocetinostat 1.0000 2.6667
uM
 48
hr
 2 533 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 254.36 NGFR rabbit IgG(H+L) / donkey NGFR 81.09
A375-A2-rep2
E4
COLO 858 Vemurafenib BIX-01294 0.3160 2.6667
uM
 48
hr
 1 561 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 189.27 NGFR rabbit IgG(H+L) / donkey NGFR 24.74
Colo858-A2-rep1
F5
COLO 858 Vemurafenib AGK2 0.3160 2.6667
uM
 48
hr
 1 564 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 311.41 NGFR rabbit IgG(H+L) / donkey NGFR 108.97
Colo858-A2-rep1
D5
COLO 858 Vemurafenib UNC0638 0.3160 2.6667
uM
 48
hr
 1 581 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 215.25 NGFR rabbit IgG(H+L) / donkey NGFR 98.55
Colo858-A2-rep1
G5
WM-115 Vemurafenib Azacitidine 1.0000 2.6667
uM
 48
hr
 1 589 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 180.96 NGFR rabbit IgG(H+L) / donkey NGFR 483.03
WM115-A2-rep1
E2
COLO 858 Vemurafenib Rocilinostat 0.3160 2.6667
uM
 48
hr
 2 596 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 175.51 NGFR rabbit IgG(H+L) / donkey NGFR 157.64
Colo858-A2-rep2
B5
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