Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Vorinostat 1.0000 0.1067
uM
 48
hr
 1 2971 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 321.51 NGFR rabbit IgG(H+L) / donkey NGFR 148.00
A375-B2-rep1
D2
A-375 Vemurafenib C646 1.0000 0.1067
uM
 48
hr
 1 2669 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 187.99 NGFR rabbit IgG(H+L) / donkey NGFR 202.06
A375-B2-rep1
E3
A-375 Vemurafenib UNC669 1.0000 0.1067
uM
 48
hr
 1 2260 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 166.44 NGFR rabbit IgG(H+L) / donkey NGFR 151.61
A375-B2-rep1
B8
A-375 Vemurafenib UNC1215 1.0000 0.1067
uM
 48
hr
 1 2816 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 174.63 NGFR rabbit IgG(H+L) / donkey NGFR 221.52
A375-B2-rep1
C8
A-375 Vemurafenib GSK-J4 1.0000 0.1067
uM
 48
hr
 1 3386 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.57 NGFR rabbit IgG(H+L) / donkey NGFR 228.63
A375-B2-rep1
D6
A-375 Vemurafenib Azacitidine 1.0000 0.1067
uM
 48
hr
 1 2464 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 196.92 NGFR rabbit IgG(H+L) / donkey NGFR 145.59
A375-B2-rep1
E2
A-375 Vemurafenib CTB 1.0000 0.1067
uM
 48
hr
 1 2800 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.07 NGFR rabbit IgG(H+L) / donkey NGFR 173.92
A375-B2-rep1
B4
A-375 Vemurafenib PFI-1 1.0000 0.1067
uM
 48
hr
 1 2527 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 190.19 NGFR rabbit IgG(H+L) / donkey NGFR 218.09
A375-B2-rep1
B7
A-375 Vemurafenib Belinostat 1.0000 0.1067
uM
 48
hr
 1 2949 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 194.46 NGFR rabbit IgG(H+L) / donkey NGFR 37.06
A375-B2-rep1
C4
A-375 Vemurafenib (+)-JQ1 1.0000 0.1067
uM
 48
hr
 1 2815 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.87 NGFR rabbit IgG(H+L) / donkey NGFR 142.58
A375-B2-rep1
C7
A-375 Vemurafenib Entinostat 1.0000 0.1067
uM
 48
hr
 1 3329 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 267.58 NGFR rabbit IgG(H+L) / donkey NGFR 296.57
A375-B2-rep1
D4
A-375 Vemurafenib AGK2 1.0000 0.1067
uM
 48
hr
 1 3383 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 234.29 NGFR rabbit IgG(H+L) / donkey NGFR 181.56
A375-B2-rep1
D5
A-375 Vemurafenib (-)-JQ1 1.0000 0.1067
uM
 48
hr
 1 3182 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 171.84 NGFR rabbit IgG(H+L) / donkey NGFR 203.99
A375-B2-rep1
D7
A-375 Vemurafenib IOX2 1.0000 0.1067
uM
 48
hr
 1 3139 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 180.23 NGFR rabbit IgG(H+L) / donkey NGFR 204.69
A375-B2-rep1
D8
A-375 Vemurafenib Olaparib 1.0000 0.1067
uM
 48
hr
 1 2585 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 174.02 NGFR rabbit IgG(H+L) / donkey NGFR 185.93
A375-B2-rep1
B2
A-375 Vemurafenib Iniparib 1.0000 0.1067
uM
 48
hr
 1 2591 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.78 NGFR rabbit IgG(H+L) / donkey NGFR 183.97
A375-B2-rep1
B3
A-375 Vemurafenib Rocilinostat 1.0000 0.1067
uM
 48
hr
 1 2966 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 230.12 NGFR rabbit IgG(H+L) / donkey NGFR 190.47
A375-B2-rep1
B5
A-375 Vemurafenib GSK-J1 1.0000 0.1067
uM
 48
hr
 1 2690 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 178.62 NGFR rabbit IgG(H+L) / donkey NGFR 187.40
A375-B2-rep1
B6
A-375 Vemurafenib Veliparib 1.0000 0.1067
uM
 48
hr
 1 2645 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.72 NGFR rabbit IgG(H+L) / donkey NGFR 170.56
A375-B2-rep1
C2
A-375 Vemurafenib Rucaparib 1.0000 0.1067
uM
 48
hr
 1 2454 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 162.10 NGFR rabbit IgG(H+L) / donkey NGFR 185.00
A375-B2-rep1
C3
A-375 Vemurafenib Selisistat 1.0000 0.1067
uM
 48
hr
 1 3138 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 229.27 NGFR rabbit IgG(H+L) / donkey NGFR 239.03
A375-B2-rep1
C5
A-375 Vemurafenib GSK-J2 1.0000 0.1067
uM
 48
hr
 1 3266 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 196.57 NGFR rabbit IgG(H+L) / donkey NGFR 202.00
A375-B2-rep1
C6
A-375 Vemurafenib JW55 1.0000 0.1067
uM
 48
hr
 1 2745 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 171.36 NGFR rabbit IgG(H+L) / donkey NGFR 189.88
A375-B2-rep1
D3
A-375 Vemurafenib Resveratrol 1.0000 0.1067
uM
 48
hr
 1 2946 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 177.17 NGFR rabbit IgG(H+L) / donkey NGFR 188.00
A375-B2-rep1
E5
A-375 Vemurafenib Mocetinostat 1.0000 0.1067
uM
 48
hr
 1 2929 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 280.55 NGFR rabbit IgG(H+L) / donkey NGFR 517.04
A375-B2-rep1
E4
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