Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -3.29 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 92.30
WM115-P2
E5
WM-115 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -5.25 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 68.74
WM115-P2
E6
WM-115 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 80.75 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 73.82
WM115-P2
E7
WM-115 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 117.95 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 206.31
WM115-P2
E8
WM-115 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 52.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 214.91
WM115-P2
E9
WM-115 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 6.69 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 215.25
WM115-P2
F10
WM-115 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -43.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 155.33
WM115-P2
F11
WM-115 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 226.91 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 86.20
WM115-P2
F2
WM-115 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 182.08 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 191.61
WM115-P2
F3
WM-115 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 100.26 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 221.21
WM115-P2
F4
WM-115 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 44.76 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 186.09
WM115-P2
F5
WM-115 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -2.79 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 142.13
WM115-P2
F6
WM-115 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 109.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 64.88
WM115-P2
F7
WM-115 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 99.09 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 199.40
WM115-P2
F8
WM-115 Vemurafenib I-BET151 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 29.33 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 222.32
WM115-P2
F9
WM-115 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -10.72 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 215.92
WM115-P2
G10
WM-115 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -45.64 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 164.62
WM115-P2
G11
WM-115 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.46 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 61.74
WM115-P2
G2
WM-115 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.10 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 167.44
WM115-P2
G3
WM-115 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 86.38 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 198.34
WM115-P2
G4
WM-115 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 64.68 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 186.39
WM115-P2
G5
WM-115 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 62.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 154.19
WM115-P2
G6
WM-115 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 151.39 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 66.24
WM115-P2
G7
WM-115 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.97 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 191.43
WM115-P2
G8
WM-115 Vemurafenib I-BET151 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 39.49 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 222.64
WM115-P2
G9