Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 20.81 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 192.06
WM115-P2
C10
WM-115 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -24.55 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 171.49
WM115-P2
C11
WM-115 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 324.06 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 110.35
WM115-P2
C2
WM-115 Vemurafenib 0.100
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 237.92 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 209.32
WM115-P2
C3
WM-115 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 177.49 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 242.39
WM115-P2
C4
WM-115 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 91.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 223.95
WM115-P2
C5
WM-115 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 15.97 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 178.14
WM115-P2
C6
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 34.61 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 205.10
WM115-P2
C7
WM-115 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 50.81 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 209.49
WM115-P2
C8
WM-115 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 41.21 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 204.84
WM115-P2
C9
WM-115 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 39.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 219.68
WM115-P2
D10
WM-115 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3.52 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 195.37
WM115-P2
D11
WM-115 Vemurafenib Belinostat 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 177.14 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 71.20
WM115-P2
D2
WM-115 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.48 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 96.75
WM115-P2
D3
WM-115 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 93.62 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 112.86
WM115-P2
D4
WM-115 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 29.37 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 102.69
WM115-P2
D5
WM-115 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -22.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 68.18
WM115-P2
D6
WM-115 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 73.97 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 66.16
WM115-P2
D7
WM-115 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 108.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 208.22
WM115-P2
D8
WM-115 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 43.98 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 217.65
WM115-P2
D9
WM-115 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 29.77 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 225.05
WM115-P2
E10
WM-115 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -32.79 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 171.27
WM115-P2
E11
WM-115 Vemurafenib Belinostat 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.79 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 70.65
WM115-P2
E2
WM-115 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 96.42 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 84.25
WM115-P2
E3
WM-115 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 79.46 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 106.82
WM115-P2
E4