Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 224.45 NGFR rabbit IgG(H+L) / donkey NGFR 168.67
Colo858-P3
C5
COLO 858 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 212.43 NGFR rabbit IgG(H+L) / donkey NGFR 163.78
Colo858-P3
C6
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 183.04 NGFR rabbit IgG(H+L) / donkey NGFR 434.11
Colo858-P3
C7
COLO 858 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 180.80 NGFR rabbit IgG(H+L) / donkey NGFR 421.39
Colo858-P3
C8
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 210.18 NGFR rabbit IgG(H+L) / donkey NGFR 376.06
Colo858-P3
C9
COLO 858 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 158.50 NGFR rabbit IgG(H+L) / donkey NGFR 28.79
Colo858-P3
D10
COLO 858 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 170.61 NGFR rabbit IgG(H+L) / donkey NGFR 19.72
Colo858-P3
D11
COLO 858 Vemurafenib Belinostat 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 370.23 NGFR rabbit IgG(H+L) / donkey NGFR 208.01
Colo858-P3
D2
COLO 858 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 353.01 NGFR rabbit IgG(H+L) / donkey NGFR 115.83
Colo858-P3
D3
COLO 858 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 272.91 NGFR rabbit IgG(H+L) / donkey NGFR 103.52
Colo858-P3
D4
COLO 858 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 220.94 NGFR rabbit IgG(H+L) / donkey NGFR 94.00
Colo858-P3
D5
COLO 858 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.00 NGFR rabbit IgG(H+L) / donkey NGFR 85.45
Colo858-P3
D6
COLO 858 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 941.81 NGFR rabbit IgG(H+L) / donkey NGFR 38.28
Colo858-P3
D7
COLO 858 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 200.20 NGFR rabbit IgG(H+L) / donkey NGFR 32.97
Colo858-P3
D8
COLO 858 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 186.47 NGFR rabbit IgG(H+L) / donkey NGFR 36.25
Colo858-P3
D9
COLO 858 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 170.61 NGFR rabbit IgG(H+L) / donkey NGFR 25.12
Colo858-P3
E10
COLO 858 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.97 NGFR rabbit IgG(H+L) / donkey NGFR 20.97
Colo858-P3
E11
COLO 858 Vemurafenib Belinostat 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.69 NGFR rabbit IgG(H+L) / donkey NGFR 196.85
Colo858-P3
E2
COLO 858 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 271.17 NGFR rabbit IgG(H+L) / donkey NGFR 87.34
Colo858-P3
E3
COLO 858 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 263.93 NGFR rabbit IgG(H+L) / donkey NGFR 90.83
Colo858-P3
E4
COLO 858 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 176.07 NGFR rabbit IgG(H+L) / donkey NGFR 75.51
Colo858-P3
E5
COLO 858 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 211.72 NGFR rabbit IgG(H+L) / donkey NGFR 68.81
Colo858-P3
E6
COLO 858 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 934.88 NGFR rabbit IgG(H+L) / donkey NGFR 31.40
Colo858-P3
E7
COLO 858 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 200.64 NGFR rabbit IgG(H+L) / donkey NGFR 30.19
Colo858-P3
E8
COLO 858 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.79 NGFR rabbit IgG(H+L) / donkey NGFR 30.47
Colo858-P3
E9