Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 218.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 228.57
Colo858-P2
G10
COLO 858 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.08 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 211.05
Colo858-P2
G11
COLO 858 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 451.17 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 96.64
Colo858-P2
G2
COLO 858 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 330.58 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 227.73
Colo858-P2
G3
COLO 858 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 290.48 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 207.76
Colo858-P2
G4
COLO 858 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 347.98 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 214.70
Colo858-P2
G5
COLO 858 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 314.66 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 290.86
Colo858-P2
G6
COLO 858 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 690.88 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 98.91
Colo858-P2
G7
COLO 858 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 283.48 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 217.07
Colo858-P2
G8
COLO 858 Vemurafenib I-BET151 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 236.94 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 187.54
Colo858-P2
G9
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.30 NGFR rabbit IgG(H+L) / donkey NGFR 320.04
Colo858-P3
B10
COLO 858 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 102.66 NGFR rabbit IgG(H+L) / donkey NGFR 69.04
Colo858-P3
B11
COLO 858 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1403.63 NGFR rabbit IgG(H+L) / donkey NGFR 64.31
Colo858-P3
B2
COLO 858 Vemurafenib 0.100
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 746.64 NGFR rabbit IgG(H+L) / donkey NGFR 71.65
Colo858-P3
B3
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 391.25 NGFR rabbit IgG(H+L) / donkey NGFR 105.88
Colo858-P3
B4
COLO 858 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 300.75 NGFR rabbit IgG(H+L) / donkey NGFR 154.41
Colo858-P3
B5
COLO 858 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.83 NGFR rabbit IgG(H+L) / donkey NGFR 257.33
Colo858-P3
B6
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 205.32 NGFR rabbit IgG(H+L) / donkey NGFR 494.57
Colo858-P3
B7
COLO 858 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 251.91 NGFR rabbit IgG(H+L) / donkey NGFR 400.92
Colo858-P3
B8
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 233.02 NGFR rabbit IgG(H+L) / donkey NGFR 334.35
Colo858-P3
B9
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 188.02 NGFR rabbit IgG(H+L) / donkey NGFR 382.19
Colo858-P3
C10
COLO 858 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 77.68 NGFR rabbit IgG(H+L) / donkey NGFR 102.40
Colo858-P3
C11
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1397.59 NGFR rabbit IgG(H+L) / donkey NGFR 52.78
Colo858-P3
C2
COLO 858 Vemurafenib 0.100
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 879.72 NGFR rabbit IgG(H+L) / donkey NGFR 71.30
Colo858-P3
C3
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 440.48 NGFR rabbit IgG(H+L) / donkey NGFR 107.94
Colo858-P3
C4