Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.17 NGFR rabbit IgG(H+L) / donkey NGFR 653.89
A375-P3
B5
A-375 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.56 NGFR rabbit IgG(H+L) / donkey NGFR 435.84
A375-P3
B6
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 100.84 NGFR rabbit IgG(H+L) / donkey NGFR 788.23
A375-P3
B7
A-375 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.16 NGFR rabbit IgG(H+L) / donkey NGFR 712.13
A375-P3
B8
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 63.33 NGFR rabbit IgG(H+L) / donkey NGFR 731.89
A375-P3
B9
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 46.71 NGFR rabbit IgG(H+L) / donkey NGFR 585.27
A375-P3
C10
A-375 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -3.64 NGFR rabbit IgG(H+L) / donkey NGFR 380.40
A375-P3
C11
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1265.78 NGFR rabbit IgG(H+L) / donkey NGFR 358.34
A375-P3
C2
A-375 Vemurafenib 0.100
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 488.72 NGFR rabbit IgG(H+L) / donkey NGFR 484.00
A375-P3
C3
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 311.66 NGFR rabbit IgG(H+L) / donkey NGFR 695.43
A375-P3
C4
A-375 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.75 NGFR rabbit IgG(H+L) / donkey NGFR 648.96
A375-P3
C5
A-375 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 52.16 NGFR rabbit IgG(H+L) / donkey NGFR 413.53
A375-P3
C6
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 70.23 NGFR rabbit IgG(H+L) / donkey NGFR 759.36
A375-P3
C7
A-375 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 92.75 NGFR rabbit IgG(H+L) / donkey NGFR 779.46
A375-P3
C8
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 81.62 NGFR rabbit IgG(H+L) / donkey NGFR 745.88
A375-P3
C9
A-375 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 72.95 NGFR rabbit IgG(H+L) / donkey NGFR 360.57
A375-P3
D10
A-375 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 12.71 NGFR rabbit IgG(H+L) / donkey NGFR 168.79
A375-P3
D11
A-375 Vemurafenib Belinostat 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 273.73 NGFR rabbit IgG(H+L) / donkey NGFR 66.64
A375-P3
D2
A-375 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 160.11 NGFR rabbit IgG(H+L) / donkey NGFR 43.39
A375-P3
D3
A-375 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 88.14 NGFR rabbit IgG(H+L) / donkey NGFR 33.82
A375-P3
D4
A-375 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 64.77 NGFR rabbit IgG(H+L) / donkey NGFR 43.50
A375-P3
D5
A-375 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 4.70 NGFR rabbit IgG(H+L) / donkey NGFR 24.69
A375-P3
D6
A-375 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 790.67 NGFR rabbit IgG(H+L) / donkey NGFR 252.85
A375-P3
D7
A-375 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 255.92 NGFR rabbit IgG(H+L) / donkey NGFR 159.75
A375-P3
D8
A-375 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.28 NGFR rabbit IgG(H+L) / donkey NGFR 284.83
A375-P3
D9