Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Selisistat 1.0000 0.1067
uM
 48
hr
 2 2645 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 169.06 NGFR rabbit IgG(H+L) / donkey NGFR 179.21
A375-B2-rep2
C5
A-375 Vemurafenib GSK-J2 1.0000 0.1067
uM
 48
hr
 2 2747 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 160.01 NGFR rabbit IgG(H+L) / donkey NGFR 157.50
A375-B2-rep2
C6
A-375 Vemurafenib (+)-JQ1 1.0000 0.1067
uM
 48
hr
 2 2498 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.23 NGFR rabbit IgG(H+L) / donkey NGFR 95.78
A375-B2-rep2
C7
A-375 Vemurafenib UNC1215 1.0000 0.1067
uM
 48
hr
 2 2603 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.59 NGFR rabbit IgG(H+L) / donkey NGFR 163.76
A375-B2-rep2
C8
A-375 Vemurafenib Vorinostat 1.0000 0.1067
uM
 48
hr
 2 2959 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.98 NGFR rabbit IgG(H+L) / donkey NGFR 129.76
A375-B2-rep2
D2
A-375 Vemurafenib JW55 1.0000 0.1067
uM
 48
hr
 2 2703 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.09 NGFR rabbit IgG(H+L) / donkey NGFR 169.94
A375-B2-rep2
D3
A-375 Vemurafenib Entinostat 1.0000 0.1067
uM
 48
hr
 2 3256 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 245.92 NGFR rabbit IgG(H+L) / donkey NGFR 251.65
A375-B2-rep2
D4
A-375 Vemurafenib AGK2 1.0000 0.1067
uM
 48
hr
 2 3264 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 185.18 NGFR rabbit IgG(H+L) / donkey NGFR 175.19
A375-B2-rep2
D5
A-375 Vemurafenib GSK-J4 1.0000 0.1067
uM
 48
hr
 2 3245 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 160.22 NGFR rabbit IgG(H+L) / donkey NGFR 162.74
A375-B2-rep2
D6
A-375 Vemurafenib (-)-JQ1 1.0000 0.1067
uM
 48
hr
 2 2939 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 160.07 NGFR rabbit IgG(H+L) / donkey NGFR 187.46
A375-B2-rep2
D7
A-375 Vemurafenib IOX2 1.0000 0.1067
uM
 48
hr
 2 2777 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.74 NGFR rabbit IgG(H+L) / donkey NGFR 180.49
A375-B2-rep2
D8
A-375 Vemurafenib Azacitidine 1.0000 0.1067
uM
 48
hr
 2 2202 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.55 NGFR rabbit IgG(H+L) / donkey NGFR 137.51
A375-B2-rep2
E2
A-375 Vemurafenib C646 1.0000 0.1067
uM
 48
hr
 2 2676 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 166.48 NGFR rabbit IgG(H+L) / donkey NGFR 161.93
A375-B2-rep2
E3
A-375 Vemurafenib Mocetinostat 1.0000 0.1067
uM
 48
hr
 2 3376 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 267.71 NGFR rabbit IgG(H+L) / donkey NGFR 465.75
A375-B2-rep2
E4
A-375 Vemurafenib Resveratrol 1.0000 0.1067
uM
 48
hr
 2 2986 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.20 NGFR rabbit IgG(H+L) / donkey NGFR 175.26
A375-B2-rep2
E5
A-375 Vemurafenib Daminozide 1.0000 0.1067
uM
 48
hr
 2 3195 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 177.92 NGFR rabbit IgG(H+L) / donkey NGFR 177.56
A375-B2-rep2
E6
A-375 Vemurafenib I-BET 1.0000 0.1067
uM
 48
hr
 2 2858 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.80 NGFR rabbit IgG(H+L) / donkey NGFR 167.95
A375-B2-rep2
E7
A-375 Vemurafenib Epigallocatechin gallate 1.0000 0.1067
uM
 48
hr
 2 2944 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 192.74 NGFR rabbit IgG(H+L) / donkey NGFR 163.13
A375-B2-rep2
E8
A-375 Vemurafenib Decitabine 1.0000 0.1067
uM
 48
hr
 2 2067 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 158.72 NGFR rabbit IgG(H+L) / donkey NGFR 148.91
A375-B2-rep2
F2
A-375 Vemurafenib Garcinol 1.0000 0.1067
uM
 48
hr
 2 2577 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 178.61 NGFR rabbit IgG(H+L) / donkey NGFR 168.67
A375-B2-rep2
F3
A-375 Vemurafenib Pracinostat 1.0000 0.1067
uM
 48
hr
 2 3139 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 547.64 NGFR rabbit IgG(H+L) / donkey NGFR 127.96
A375-B2-rep2
F4
A-375 Vemurafenib BIX-01294 1.0000 0.1067
uM
 48
hr
 2 2538 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.02 NGFR rabbit IgG(H+L) / donkey NGFR 153.89
A375-B2-rep2
F5
A-375 Vemurafenib Methylstat 1.0000 0.1067
uM
 48
hr
 2 2638 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.17 NGFR rabbit IgG(H+L) / donkey NGFR 152.99
A375-B2-rep2
F6
A-375 Vemurafenib I-BET151 1.0000 0.1067
uM
 48
hr
 2 2822 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.62 NGFR rabbit IgG(H+L) / donkey NGFR 182.94
A375-B2-rep2
F7
A-375 Vemurafenib CX-5461 1.0000 0.1067
uM
 48
hr
 2 1802 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.80 NGFR rabbit IgG(H+L) / donkey NGFR 134.98
A375-B2-rep2
F8