Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib GSK-J1 1.0000 2.6667
uM
 48
hr
 2 2734 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 198.78 NGFR rabbit IgG(H+L) / donkey NGFR 231.75
A375-A2-rep2
B6
A-375 Vemurafenib PFI-1 1.0000 2.6667
uM
 48
hr
 2 2464 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.44 NGFR rabbit IgG(H+L) / donkey NGFR 154.43
A375-A2-rep2
B7
A-375 Vemurafenib UNC669 1.0000 2.6667
uM
 48
hr
 2 2373 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 210.68 NGFR rabbit IgG(H+L) / donkey NGFR 209.48
A375-A2-rep2
B8
A-375 Vemurafenib Veliparib 1.0000 2.6667
uM
 48
hr
 2 2511 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 190.08 NGFR rabbit IgG(H+L) / donkey NGFR 181.11
A375-A2-rep2
C2
A-375 Vemurafenib Rucaparib 1.0000 2.6667
uM
 48
hr
 2 2477 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.42 NGFR rabbit IgG(H+L) / donkey NGFR 200.11
A375-A2-rep2
C3
A-375 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 2 319 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 360.58 NGFR rabbit IgG(H+L) / donkey NGFR 26.94
A375-A2-rep2
C4
A-375 Vemurafenib Selisistat 1.0000 2.6667
uM
 48
hr
 2 3238 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 195.56 NGFR rabbit IgG(H+L) / donkey NGFR 217.60
A375-A2-rep2
C5
A-375 Vemurafenib GSK-J2 1.0000 2.6667
uM
 48
hr
 2 3289 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 175.33 NGFR rabbit IgG(H+L) / donkey NGFR 210.26
A375-A2-rep2
C6
A-375 Vemurafenib (+)-JQ1 1.0000 2.6667
uM
 48
hr
 2 2251 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 165.40 NGFR rabbit IgG(H+L) / donkey NGFR 86.81
A375-A2-rep2
C7
A-375 Vemurafenib UNC1215 1.0000 2.6667
uM
 48
hr
 2 2981 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 185.94 NGFR rabbit IgG(H+L) / donkey NGFR 204.11
A375-A2-rep2
C8
A-375 Vemurafenib Vorinostat 1.0000 2.6667
uM
 48
hr
 2 2222 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 252.58 NGFR rabbit IgG(H+L) / donkey NGFR 61.42
A375-A2-rep2
D2
A-375 Vemurafenib JW55 1.0000 2.6667
uM
 48
hr
 2 2911 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.15 NGFR rabbit IgG(H+L) / donkey NGFR 159.22
A375-A2-rep2
D3
A-375 Vemurafenib Entinostat 1.0000 2.6667
uM
 48
hr
 2 3308 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 589.21 NGFR rabbit IgG(H+L) / donkey NGFR 306.13
A375-A2-rep2
D4
A-375 Vemurafenib AGK2 1.0000 2.6667
uM
 48
hr
 2 1329 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 425.48 NGFR rabbit IgG(H+L) / donkey NGFR 176.32
A375-A2-rep2
D5
A-375 Vemurafenib GSK-J4 1.0000 2.6667
uM
 48
hr
 2 1738 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 131.40 NGFR rabbit IgG(H+L) / donkey NGFR 104.43
A375-A2-rep2
D6
A-375 Vemurafenib (-)-JQ1 1.0000 2.6667
uM
 48
hr
 2 3409 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 167.33 NGFR rabbit IgG(H+L) / donkey NGFR 293.21
A375-A2-rep2
D7
A-375 Vemurafenib IOX2 1.0000 2.6667
uM
 48
hr
 2 2998 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 182.38 NGFR rabbit IgG(H+L) / donkey NGFR 220.87
A375-A2-rep2
D8
A-375 Vemurafenib Azacitidine 1.0000 2.6667
uM
 48
hr
 2 1303 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 286.33 NGFR rabbit IgG(H+L) / donkey NGFR 62.73
A375-A2-rep2
E2
A-375 Vemurafenib C646 1.0000 2.6667
uM
 48
hr
 2 2465 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 167.50 NGFR rabbit IgG(H+L) / donkey NGFR 173.88
A375-A2-rep2
E3
A-375 Vemurafenib Mocetinostat 1.0000 2.6667
uM
 48
hr
 2 533 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 254.36 NGFR rabbit IgG(H+L) / donkey NGFR 81.09
A375-A2-rep2
E4
A-375 Vemurafenib Resveratrol 1.0000 2.6667
uM
 48
hr
 2 2903 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 213.67 NGFR rabbit IgG(H+L) / donkey NGFR 191.15
A375-A2-rep2
E5
A-375 Vemurafenib Daminozide 1.0000 2.6667
uM
 48
hr
 2 2889 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.32 NGFR rabbit IgG(H+L) / donkey NGFR 208.04
A375-A2-rep2
E6
A-375 Vemurafenib I-BET 1.0000 2.6667
uM
 48
hr
 2 2493 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.30 NGFR rabbit IgG(H+L) / donkey NGFR 93.88
A375-A2-rep2
E7
A-375 Vemurafenib Epigallocatechin gallate 1.0000 2.6667
uM
 48
hr
 2 2588 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 185.72 NGFR rabbit IgG(H+L) / donkey NGFR 175.22
A375-A2-rep2
E8
A-375 Vemurafenib Decitabine 1.0000 2.6667
uM
 48
hr
 2 2092 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 222.42 NGFR rabbit IgG(H+L) / donkey NGFR 140.13
A375-A2-rep2
F2