Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib RG-108 1.0000 0.1067
uM
 48
hr
 2 2074 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.49 NGFR rabbit IgG(H+L) / donkey NGFR 139.25
A375-B2-rep2
G2
A-375 Vemurafenib Anacardic acid 1.0000 0.1067
uM
 48
hr
 2 2049 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 170.49 NGFR rabbit IgG(H+L) / donkey NGFR 140.28
A375-B2-rep2
G3
A-375 Vemurafenib MC1568 1.0000 0.1067
uM
 48
hr
 2 2119 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 209.27 NGFR rabbit IgG(H+L) / donkey NGFR 157.16
A375-B2-rep2
G4
A-375 Vemurafenib UNC0638 1.0000 0.1067
uM
 48
hr
 2 2188 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 167.32 NGFR rabbit IgG(H+L) / donkey NGFR 140.34
A375-B2-rep2
G5
A-375 Vemurafenib Tranylcypromine 1.0000 0.1067
uM
 48
hr
 2 1897 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.61 NGFR rabbit IgG(H+L) / donkey NGFR 138.48
A375-B2-rep2
G6
A-375 Vemurafenib Ischemin 1.0000 0.1067
uM
 48
hr
 2 2019 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.59 NGFR rabbit IgG(H+L) / donkey NGFR 146.24
A375-B2-rep2
G7
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1485 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.40 NGFR rabbit IgG(H+L) / donkey NGFR 124.35
A375-B2-rep2
H10
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1564 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.45 NGFR rabbit IgG(H+L) / donkey NGFR 110.35
A375-B2-rep2
H11
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1626 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 158.56 NGFR rabbit IgG(H+L) / donkey NGFR 142.15
A375-B2-rep2
H2
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1568 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.46 NGFR rabbit IgG(H+L) / donkey NGFR 132.48
A375-B2-rep2
H3
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1560 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.12 NGFR rabbit IgG(H+L) / donkey NGFR 135.12
A375-B2-rep2
H4
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1377 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.79 NGFR rabbit IgG(H+L) / donkey NGFR 130.22
A375-B2-rep2
H5
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1391 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.37 NGFR rabbit IgG(H+L) / donkey NGFR 133.06
A375-B2-rep2
H6
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1348 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 158.69 NGFR rabbit IgG(H+L) / donkey NGFR 125.38
A375-B2-rep2
H7
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1439 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.21 NGFR rabbit IgG(H+L) / donkey NGFR 140.69
A375-B2-rep2
H8
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1514 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.99 NGFR rabbit IgG(H+L) / donkey NGFR 137.41
A375-B2-rep2
H9
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1391 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.32 NGFR rabbit IgG(H+L) / donkey NGFR 158.87
Colo858-A2-rep1
A10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1242 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 296.85 NGFR rabbit IgG(H+L) / donkey NGFR 156.51
Colo858-A2-rep1
A11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1202 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 347.86 NGFR rabbit IgG(H+L) / donkey NGFR 141.29
Colo858-A2-rep1
A2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1024 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.50 NGFR rabbit IgG(H+L) / donkey NGFR 135.78
Colo858-A2-rep1
A3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1076 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 288.18 NGFR rabbit IgG(H+L) / donkey NGFR 162.29
Colo858-A2-rep1
A4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1122 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 296.79 NGFR rabbit IgG(H+L) / donkey NGFR 157.80
Colo858-A2-rep1
A5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 920 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 297.13 NGFR rabbit IgG(H+L) / donkey NGFR 146.65
Colo858-A2-rep1
A6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1095 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 337.39 NGFR rabbit IgG(H+L) / donkey NGFR 164.33
Colo858-A2-rep1
A7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 897 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 335.21 NGFR rabbit IgG(H+L) / donkey NGFR 126.80
Colo858-A2-rep1
A8