Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Entinostat 0.3160 0.5333
uM
 48
hr
 2 2701 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 211.88 NGFR rabbit IgG(H+L) / donkey NGFR 80.70
Colo858-B1-rep2
D4
COLO 858 Vemurafenib AGK2 0.3160 0.5333
uM
 48
hr
 2 1820 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 307.04 NGFR rabbit IgG(H+L) / donkey NGFR 114.96
Colo858-B1-rep2
D5
COLO 858 Vemurafenib GSK-J4 0.3160 0.5333
uM
 48
hr
 2 3044 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 288.92 NGFR rabbit IgG(H+L) / donkey NGFR 131.62
Colo858-B1-rep2
D6
COLO 858 Vemurafenib (-)-JQ1 0.3160 0.5333
uM
 48
hr
 2 3387 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 288.83 NGFR rabbit IgG(H+L) / donkey NGFR 150.04
Colo858-B1-rep2
D7
COLO 858 Vemurafenib IOX2 0.3160 0.5333
uM
 48
hr
 2 3074 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 323.98 NGFR rabbit IgG(H+L) / donkey NGFR 143.82
Colo858-B1-rep2
D8
COLO 858 Vemurafenib Azacitidine 0.3160 0.5333
uM
 48
hr
 2 1199 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 281.80 NGFR rabbit IgG(H+L) / donkey NGFR 95.43
Colo858-B1-rep2
E2
COLO 858 Vemurafenib C646 0.3160 0.5333
uM
 48
hr
 2 2126 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 248.60 NGFR rabbit IgG(H+L) / donkey NGFR 140.35
Colo858-B1-rep2
E3
COLO 858 Vemurafenib Mocetinostat 0.3160 0.5333
uM
 48
hr
 2 2847 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.05 NGFR rabbit IgG(H+L) / donkey NGFR 41.69
Colo858-B1-rep2
E4
COLO 858 Vemurafenib Resveratrol 0.3160 0.5333
uM
 48
hr
 2 2772 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 301.42 NGFR rabbit IgG(H+L) / donkey NGFR 139.46
Colo858-B1-rep2
E5
COLO 858 Vemurafenib Daminozide 0.3160 0.5333
uM
 48
hr
 2 3157 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 329.58 NGFR rabbit IgG(H+L) / donkey NGFR 139.83
Colo858-B1-rep2
E6
COLO 858 Vemurafenib I-BET 0.3160 0.5333
uM
 48
hr
 2 2224 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.45 NGFR rabbit IgG(H+L) / donkey NGFR 24.73
Colo858-B1-rep2
E7
COLO 858 Vemurafenib Epigallocatechin gallate 0.3160 0.5333
uM
 48
hr
 2 2802 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 324.58 NGFR rabbit IgG(H+L) / donkey NGFR 137.24
Colo858-B1-rep2
E8
COLO 858 Vemurafenib Decitabine 0.3160 0.5333
uM
 48
hr
 2 1367 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 214.98 NGFR rabbit IgG(H+L) / donkey NGFR 173.73
Colo858-B1-rep2
F2
COLO 858 Vemurafenib Garcinol 0.3160 0.5333
uM
 48
hr
 2 1674 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 281.10 NGFR rabbit IgG(H+L) / donkey NGFR 142.39
Colo858-B1-rep2
F3
COLO 858 Vemurafenib Pracinostat 0.3160 0.5333
uM
 48
hr
 2 326 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 166.82 NGFR rabbit IgG(H+L) / donkey NGFR 26.66
Colo858-B1-rep2
F4
COLO 858 Vemurafenib BIX-01294 0.3160 0.5333
uM
 48
hr
 2 1928 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.98 NGFR rabbit IgG(H+L) / donkey NGFR 120.70
Colo858-B1-rep2
F5
COLO 858 Vemurafenib Methylstat 0.3160 0.5333
uM
 48
hr
 2 2202 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 242.84 NGFR rabbit IgG(H+L) / donkey NGFR 132.23
Colo858-B1-rep2
F6
COLO 858 Vemurafenib I-BET151 0.3160 0.5333
uM
 48
hr
 2 1692 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 132.87 NGFR rabbit IgG(H+L) / donkey NGFR 15.18
Colo858-B1-rep2
F7
COLO 858 Vemurafenib CX-5461 0.3160 0.5333
uM
 48
hr
 2 1972 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 210.24 NGFR rabbit IgG(H+L) / donkey NGFR 218.97
Colo858-B1-rep2
F8
COLO 858 Vemurafenib RG-108 0.3160 0.5333
uM
 48
hr
 2 1025 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 269.93 NGFR rabbit IgG(H+L) / donkey NGFR 130.25
Colo858-B1-rep2
G2
COLO 858 Vemurafenib Anacardic acid 0.3160 0.5333
uM
 48
hr
 2 926 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 272.48 NGFR rabbit IgG(H+L) / donkey NGFR 124.30
Colo858-B1-rep2
G3
COLO 858 Vemurafenib MC1568 0.3160 0.5333
uM
 48
hr
 2 1098 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 323.03 NGFR rabbit IgG(H+L) / donkey NGFR 124.26
Colo858-B1-rep2
G4
COLO 858 Vemurafenib UNC0638 0.3160 0.5333
uM
 48
hr
 2 1278 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 262.32 NGFR rabbit IgG(H+L) / donkey NGFR 196.87
Colo858-B1-rep2
G5
COLO 858 Vemurafenib Tranylcypromine 0.3160 0.5333
uM
 48
hr
 2 1326 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 311.56 NGFR rabbit IgG(H+L) / donkey NGFR 177.03
Colo858-B1-rep2
G6
COLO 858 Vemurafenib Ischemin 0.3160 0.5333
uM
 48
hr
 2 1246 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 336.98 NGFR rabbit IgG(H+L) / donkey NGFR 140.12
Colo858-B1-rep2
G7