Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 994 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 353.13 NGFR rabbit IgG(H+L) / donkey NGFR 159.64
Colo858-B1-rep2
A11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 906 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 347.23 NGFR rabbit IgG(H+L) / donkey NGFR 108.59
Colo858-B1-rep2
A2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 915 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 295.91 NGFR rabbit IgG(H+L) / donkey NGFR 130.55
Colo858-B1-rep2
A3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1106 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 325.17 NGFR rabbit IgG(H+L) / donkey NGFR 144.51
Colo858-B1-rep2
A4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 856 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 298.17 NGFR rabbit IgG(H+L) / donkey NGFR 127.54
Colo858-B1-rep2
A5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 994 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 355.47 NGFR rabbit IgG(H+L) / donkey NGFR 129.93
Colo858-B1-rep2
A6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 911 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 350.80 NGFR rabbit IgG(H+L) / donkey NGFR 124.13
Colo858-B1-rep2
A7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 685 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 333.97 NGFR rabbit IgG(H+L) / donkey NGFR 138.64
Colo858-B1-rep2
A8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1238 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 334.50 NGFR rabbit IgG(H+L) / donkey NGFR 151.42
Colo858-B1-rep2
A9
COLO 858 Vemurafenib Olaparib 0.3160 0.5333
uM
 48
hr
 2 1658 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 296.94 NGFR rabbit IgG(H+L) / donkey NGFR 158.73
Colo858-B1-rep2
B2
COLO 858 Vemurafenib Iniparib 0.3160 0.5333
uM
 48
hr
 2 1475 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 300.07 NGFR rabbit IgG(H+L) / donkey NGFR 155.24
Colo858-B1-rep2
B3
COLO 858 Vemurafenib CTB 0.3160 0.5333
uM
 48
hr
 2 1443 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 290.72 NGFR rabbit IgG(H+L) / donkey NGFR 148.92
Colo858-B1-rep2
B4
COLO 858 Vemurafenib Rocilinostat 0.3160 0.5333
uM
 48
hr
 2 1194 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 240.69 NGFR rabbit IgG(H+L) / donkey NGFR 200.84
Colo858-B1-rep2
B5
COLO 858 Vemurafenib GSK-J1 0.3160 0.5333
uM
 48
hr
 2 1597 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 332.46 NGFR rabbit IgG(H+L) / donkey NGFR 143.42
Colo858-B1-rep2
B6
COLO 858 Vemurafenib PFI-1 0.3160 0.5333
uM
 48
hr
 2 1454 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 310.50 NGFR rabbit IgG(H+L) / donkey NGFR 102.50
Colo858-B1-rep2
B7
COLO 858 Vemurafenib UNC669 0.3160 0.5333
uM
 48
hr
 2 1498 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 327.22 NGFR rabbit IgG(H+L) / donkey NGFR 143.43
Colo858-B1-rep2
B8
COLO 858 Vemurafenib Veliparib 0.3160 0.5333
uM
 48
hr
 2 1765 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 252.26 NGFR rabbit IgG(H+L) / donkey NGFR 154.63
Colo858-B1-rep2
C2
COLO 858 Vemurafenib Rucaparib 0.3160 0.5333
uM
 48
hr
 2 2053 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 276.51 NGFR rabbit IgG(H+L) / donkey NGFR 162.63
Colo858-B1-rep2
C3
COLO 858 Vemurafenib Belinostat 0.3160 0.5333
uM
 48
hr
 2 367 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 240.33 NGFR rabbit IgG(H+L) / donkey NGFR 12.62
Colo858-B1-rep2
C4
COLO 858 Vemurafenib Selisistat 0.3160 0.5333
uM
 48
hr
 2 2357 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 319.34 NGFR rabbit IgG(H+L) / donkey NGFR 91.80
Colo858-B1-rep2
C5
COLO 858 Vemurafenib GSK-J2 0.3160 0.5333
uM
 48
hr
 2 2677 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 312.56 NGFR rabbit IgG(H+L) / donkey NGFR 142.25
Colo858-B1-rep2
C6
COLO 858 Vemurafenib (+)-JQ1 0.3160 0.5333
uM
 48
hr
 2 1702 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.67 NGFR rabbit IgG(H+L) / donkey NGFR 14.18
Colo858-B1-rep2
C7
COLO 858 Vemurafenib UNC1215 0.3160 0.5333
uM
 48
hr
 2 2388 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 298.81 NGFR rabbit IgG(H+L) / donkey NGFR 149.60
Colo858-B1-rep2
C8
COLO 858 Vemurafenib Vorinostat 0.3160 0.5333
uM
 48
hr
 2 1077 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 189.76 NGFR rabbit IgG(H+L) / donkey NGFR 154.45
Colo858-B1-rep2
D2
COLO 858 Vemurafenib JW55 0.3160 0.5333
uM
 48
hr
 2 2472 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 267.42 NGFR rabbit IgG(H+L) / donkey NGFR 137.02
Colo858-B1-rep2
D3