Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

 Full screen view
Filter results: >>>>
Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 572.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.89 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 121.81
WM115-P10
B5
WM-115 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 927.97 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.29 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 158.90
WM115-P10
B6
WM-115 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 931.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 176.80 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 131.68
WM115-P10
B7
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 866.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 214.45 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 72.76
WM115-P10
B8
WM-115 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 654.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 191.24 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 55.00
WM115-P10
B9
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 70.38 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 196.20
WM115-P10
C10
WM-115 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 70.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 55.30 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 164.98
WM115-P10
C11
WM-115 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 478.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 113.15 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 193.46
WM115-P10
C2
WM-115 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 401.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.52 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 171.77
WM115-P10
C3
WM-115 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 427.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.14 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 191.66
WM115-P10
C4
WM-115 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 598.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 150.95 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 200.08
WM115-P10
C5
WM-115 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 728.84 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 125.51 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 240.11
WM115-P10
C6
WM-115 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 699.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 101.58 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 189.85
WM115-P10
C7
WM-115 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 400.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 109.62 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 81.34
WM115-P10
C8
WM-115 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 291.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 80.39 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 38.02
WM115-P10
C9
WM-115 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 147.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 61.76 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 159.57
WM115-P10
D10
WM-115 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 73.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 51.63 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 142.88
WM115-P10
D11
WM-115 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 451.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 92.09 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 172.48
WM115-P10
D2
WM-115 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 436.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 107.52 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 156.01
WM115-P10
D3
WM-115 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 519.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.21 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 214.27
WM115-P10
D4
WM-115 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 502.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 143.42 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 196.58
WM115-P10
D5
WM-115 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 534.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.14 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 266.81
WM115-P10
D6
WM-115 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 646.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 124.52 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 227.41
WM115-P10
D7
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 206.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 78.96 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 51.00
WM115-P10
D8
WM-115 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 300.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 89.31 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 40.05
WM115-P10
D9